새로운 잠재혈흔 현출제로서 Diimine-type Ligand들의 잠재력
The Potential of Diimine-type Ligands as New Latent Bloodstains Detectors
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초록
새로운 잠재혈흔 현출제로서 diimine-type ligands (L, 1-7)들의 가능성을 검증하기 위하여 혈액에 대한 긍정적인 실험 결과들을 다음과 같이 얻었다. 혈흔 시료들의 탈 산소 반응에 따른 거품발생 현상은 잠재혈흔을 쉽고 간단하게 확인할 수 있는 방법중 하나였으며 적절하게 수식된 실험방법을 도출하여 리간드들의 혈흔현출 실험에 적용하였다. 리간드들의 실험결과들은 대체로 유사하였고 어두운 조건의 395 nm(연두색)에서 형광현상이 470 nm(연갈색)에서 보다 선명하였다. 또한, 2자리 리간드 화합물로서 1,10-phenanthroline 분자 그리고 Hemoglobin의 모델 화합물로서 ferrous (Fe^(2+)) chloride에 의한 착 화합물 ([Fe(L)_3)^(2+)]X^(2−))의 UV spectra를 검토하였다. 한 예로 1,10-phenanthroline의 경우, 비교적 독성이 낮음이 알려졌고, 형광현상을 장시간 동안 나타남으로 잠재혈흔에 관한 정보를 안전하고 간편하게 다룰 수 있을 것으로 판단되었다.
Abstract
To examine the potential of diimine-type ligands (L, 1-7) as new latent bloodstains detectors, the positive experimental results for blood were confirmed as stated below. The bubble phenomenon with the deoxygenation of bloodstain samples is an easy common method used to check latent bloodstains. We concluded that the modified experiment method was suitable when applied to the bloodstains detection by the ligands. The experimental results of the ligands were generally similar to that of the fluorescence phenomenon of 395nm (light yellowish green) and was clearer than those of 470nm (light brown) in a dark place. Not only that, the UV spectra of 1,10-phenanthroline molecule as bidentate ligand and its iron (II) complex ([Fe(L)_3)^(2+)]X^(2-)) with ferrous (Fe^(2+)) chloride as the model compound of hemoglobin were discussed. In the case of 1,10-phenanthroline, for example, the toxicity was known to be relatively low and the complex showed the fluorescence for a long time. Therefore, it can be regarded as a success that the results of the fluorescence phenomenon provide safe and convenient access to information on latent bloodstains from using the detectors.
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