Comparison of Bacterial Community in Soil Using Denaturing Gradient-Gel Electrophoresis and Forensic Application
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The microbial diversity can be regarded as an integral part for comparative forensic analysis of soil. This diversity may be used as a biological indicator in tracing the original location of the soil or comparing co-identity of soils. In order to evaluate this effectiveness, we examined the bacterial communities of soil by collecting soil samples from nine different sites in Daejeon and applying a 16S rRNA-based cultivation-independent approach. The formation of bacterial community in soil was more influenced by its ecosystem than its geographical location. This study provides a principal data for soil discrimination by comparative analysis of soil microbial community. If this biological analysis will be used with other physical and chemical analysis, which can be a powerful tool for comparing co-identity of soils and this will make it possible to trace the original location of the soil.
Abstract
The microbial diversity can be regarded as an integral part for comparative forensic analysis of soil. This diversity may be used as a biological indicator in tracing the original location of the soil or comparing co-identity of soils. In order to evaluate this effectiveness, we examined the bacterial communities of soil by collecting soil samples from nine different sites in Daejeon and applying a 16S rRNA-based cultivation-independent approach. The formation of bacterial community in soil was more influenced by its ecosystem than its geographical location. This study provides a principal data for soil discrimination by comparative analysis of soil microbial community. If this biological analysis will be used with other physical and chemical analysis, which can be a powerful tool for comparing co-identity of soils and this will make it possible to trace the original location of the soil.
참고문헌 (16)
- AG O'Donnell, "16S rDNA methods in soil microbiology", Current opinion in Biotechnology, 10, pp.225~229 (1999)
- J Horswell, "Forensic comparison of soils by bacterial community DNA profiling", J Forensic Sci, 47, pp.350~353 (2002)
- MS Meyers, "Spatial and temporal influences on bacterial profiling of forensic soil samples", J forensic sci, 53, pp.652~659 (2008)
- A Lerner, "Can denaturing gradient gel electrophoresis (DGGE) analysis of amplified 16s rDNA of soil bacterial populations be used in forensic investigations?", Soil Biology & Biochemistry, 38, pp.1188~1192 (2006)
- RC Murray, "Forensic science handbook. Vol 1", pp.615~633 (2002), Prentice Hall
- PA Bull, "The Forensic Analysis of Soils and Sediment Taken from the Cast of a Footprint", Forensic Sci. Int, 162, pp.6~12 (2006)
- K Pye, "Forensic comparison of soil samples: Assessment of small scale spatial variability in elemental composition, carbon and nitrogen isotope ratios, colour, and particle size distribution", Forensic Sci. Int, 163, pp.59~80 (2006)
- RJ Cox, "Forensic analysis of soil organic by FTIR", Forensic Sci. Int, 108, pp.107~116 (2000)
- RM Morgan, "Forensic Geoscience and Crime Detection", Minerva Med. Leg, 127, pp.73~89 (2007)
- Y Marumo, "Forensic Examination of Soil Evidence, Japanese J of Science and Tech", for identification, 7, pp.95~111 (2003)
- RI Griffiths, "The bacterial biogeography of British soils", Environmental Microbiology, 13, pp.1642~1654 (2011)
- V Torsvik, "Microbial phylogeny and diversity in soil, In Modern Soil Microbiology", pp.23~54 (2007), CRC Press
- M Oros-Sichler, "Molecular fingerprinting techniques to analyze soil microbial communities, In Modern Soil Microbiology", pp.355~386 (2007), CRC Press
- G Muyzer, "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA", Appl Environ Microbiol, 59, pp.695~700 (1993)
- EG Zoetendal, "Temperature Gradient Gel Electrophoresis Analysis of 16S rRNA from Human Fecal Samples Reveals Stable and Host-specific Communities of Active Bacteria", Appl Environ Microbiol, 64, pp.3854~3859 (1998)
- LE Heath, "Assessing the potential of bacterial DNA profiling of forensic soil comparisons", J Forensic Sci, 51, pp.1062~1068 (2006)