PCR-DGGE 방법을 이용한 북한강 수계 호수의 플랑크톤 군집 분석
Plankton community analysis in the lake of North-Han river system using PCR-DGGE method
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초록
식물플랑크톤의 동정은 숙련된 전문가에게도 어려운 과제이다. 별 특징없는 외형과 다양한 크기와 종은 형태학적으로 구분하기에 어려움이 있다. 본 연구에서는 미생물 군집의 다양성을 분석하는데 효과적인 fingerprinting 기법인 PCR-DGGE 방법을 사용하여 이런 형태학적 동정의 제한점을 보완하고자 하는데 목적이 있다. 5곳의 호수 샘플로부터 2008년 8월 총 46개의 band를 찾을 수 있었고, 2008년 11월 총 26개 band를 찾을 수 있었다. 이 fingerprint 결과는 각각 다른 샘플링 장소를 비교하는데 용이하였다. 본 연구에서 PCR-DGGE 방법은 북한강 호수들의 플랑크톤 군집의 다양성을 파악하는데 사용되었고, 이 DGGE 기법이 플랑크톤의 동정 기법으로써의 가능성을 검토해보았다.
Abstract
Taxonomic identification of phytoplankton has been a difficult task, even for the experienced taxonomist. Many non-descript, yet abundant, phytoplanktons do exist without distinguishing features which cause difficulties in morphological identification. Using PCR(polymerase chain reaction)-DGGE(denaturing gradient gel electrophoresis)method, which is known to be a powerful fingerprinting technique to analyze diversity and dynamics of microbial populations, this study aimed to find the way to overcome the limitation of morphological identification. As a result, a total of 46 bands from samples in five lakes were detected in September and 27 bands in November. Fingerprinting results showed convenient and comparative analyses among each sampling site. In this study, PCR-DGGE method was used to figure out diversity and dynamics of plankton community in the lakes of North-Han River system. Also, the possibility of DGGE technique as an identification tool for phytoplankton was estimated.
참고문헌 (19)
- 고소라, "DGGE를 이용한 대청호 수화 발생시기의 세균군집 분석", 미생물학회지, 40(3), pp.205~210 (2004), 한국미생물학회
- 고소라, "부영양 연못에서 초음파 작동에 따른 식물플 랑크톤의 군집 변화", 한국 환경생물학회지, 24(3), pp.221~229 (2006)
- 김범철, "소양호 동식물플랑 크톤의 계절 변동", Korean Journal of Limnology, 32(2), pp.127~134 (1999)
- 정유경, "북한강 수계 상류호수와 하류호수의 식물플랑크톤 군집 변동 : 파라호, 춘천호, 의암호, 청평호", 석사, 환경학, pp.2007
- 북한강 수계 호소의 생태계 구조조사, "한강물환경연구소", 한강물환경연구소, ~ (2007)
- Amann, R, "Phylogenetic identification and in situ detection of individual microbial cells without cultivation", Microbiological Reviews, 59, pp.143~169 (1995)
- Barth, H. J, "The influence of cyanobacteria on oil polluted intertidal soils at the Saudi Arabian Gulf shores", Marine Pollution Bulletin, 46(10), pp.1245~1252 (2003)
- Diez, B, "Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other moleculartechniques", Applied and Environmental Microbiology, 67(7), pp.2942~2951 (2001)
- Green, S. J, "A salinity and sulfate manipulation of hypersaline microbial mats reveals stasis in the cyanobacterial community structure", ISME Journal, 2(5), pp.457~470 (2008)
- Hannen, E. J, "Revealing genetic diversity of eukaryotic microorganisms in aquatic environments by Denaturing Gradient Gel Electrophoresis", Journal of Phycology, 34(2), pp.206~213 (1998)
- Muyzer, G, "Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA fragments", Applied and Environmental Microbiology, 59, pp.695~700 (1993)
- Muyzer G, "Denaturing gradient gel electrophoresis (DGGE) in microbial ecology, In Molecular Microbial Ecology Manual", pp.1~27 (1997), Kluwer Academic Publishers
- Muyzer G, "DGGE", TGGE a method for identifying genes from natural ecosystems, 1999, pp.2, 317~322
- Myers, R. M, "Methods in Enzymology", 68, pp.183~191 (1987)
- Nubel, U, "PCR primers to amplify 16S rRNA genes from cyanobacteria", Appled and Environmental Microbiology, 63(8), pp.3327~3332 (1997)
- Nubel, U, "Quantifying microbial diversity morphotypes, 16S rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats", Appled and Environmental Microbiology, 65, pp.422~430 (1999)
- Savin, M. C, "Plankton Diversity in the Bay of Fundy as Measured by Morphological and Molecular Methods", Microbial Ecology, 48(1), pp.51~65 (2004)
- Ternjej, I, "Plankton community and related environmental factors in the oligotrophic Lake Vrana", Periodicum Bilogorum, 107, pp.321~328 (2005)
- Yan, Q, "Genetic Diversity of Plankton Community as Depicted by PCR-DGGE Fingerprinting and its Relation to Morphological Composition and Environmental Factors in Lake Donghu", Microbial Ecology, 54(2), pp.290~297 (2007)