광섬유 소산장을 이용한 식중독균 신속검출용 형광면역센서
Fiber-optic fluoroimmunosensor for foodborn pathogens using an optical evanescent field
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Abstract
In this study, the fiber-optic fluoro-imunosensor was designed to detect foodborne pathogens. The fabricated systemapplied to the fabricated the fiber-optic fluoro-imunosensor. In the “sandwich” binding format, a primary or “capture”antibody is immobilized on the core surface of the multimode optical fiber and a secondary or named as “tracer” antibodyis added to the bulk solution. A tracer is labeled FITC (fluorescein isothiocyanate; λex=492 nm, λem=520 nm). Diferentconcentrations of antigens are tested in diferent fibers. The detection limit of the fabricated system is 5.08×103 cfu/mlfor Vibrio antigen and 0.1g/ml, 0.05g/ml in non-labeled monolayer phosphate bufered saline (NMP), non-labeledmonolayer carbonate bicarbonate bufer (NMC), respectively.
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