加減大黃牧丹皮湯의 급성 췌장염 억제 효과
Inhibitory effect of acute pancreatitis by Gagam-daewhangmokdanpi-tang
2
조회
0
다운로드
6
인용
0
참고문헌
초록
Abstract
Inhibitory effect of acute pancreatitis by Gagam-daewhangmokdanpi-tang Ahn Jae Sun, Lee Hai Ja, Park Eun Jung Department of Pediatrics, College of Oriental Medicine, Wonkwang Uiversity Objectives : In this study, we investigated the effects of Daehwangmokdanpitang-gagambang(DMG) on treatment for cholecystokinin-octapeptide(CCK)-induced acute pancreatitis(AP) in rats. Methods : Male Wistar rats weighing 200 to 250 g were divided into three groups. The first was normal untreated group, the second was in treatment with DMG group; DMG was administered orally, followed by 75 ㎍/㎏ CCK subcutaneously three times, after 1, 3 and 5 h. This whole procedure was repeated for 5 days. The third was in treatment with saline group, the protocol was the same as in treatment group with DMG. We determined the pancreatic weight/body weight ratio, the levels of pancreatic HSP60, HSP72, ERK, p38 MAPK and the secretion of pro-inflammatory cytokines. Repeated CCK treatment resulted in the typical laboratory and morphological changes of experimentally induced pancreatitis. Results : DMG was significantly decreased the pancreatic weight/body weight ratio in CCK- induced AP and DMG increased HSP60 and HSP72 compared with CCK-induced AP. DMG suppressed ERK and p38 MAPK activation. Additionally, the secretion of IL-1β and TNF-α and the levels of amylase and lipase were lower than that saline. Conclusions : DMG has an effect to treatment for CCK-induced AP.
참고문헌 (18)
- 이장훈, "동의임상내과학", pp.426~438 (1999), 서울: 법인문화사
- 심찬섭, "복부초음파진단학", pp.277~ (2000), 서울: 여문각
- Fink-Bennet, "Cholecystokinin cholescintigraphy:Detection of abnormal gallbladder motor fuction in patients with chronic acalculous gallbladder diseaes", pp.1695~9 (1991), Cholecystokinin cholescintigraphy
- Krishnamurthy GT, "A tecknique for quantitative analysis of motor function of the human gallbladder", pp.482~90 (1981)
- Rakonczay Z. Jr,, "Water immersion pretreatment decreases pro-inflammatory cytokine production in cholecystokinin-octapeptide-induced acute pancreatitis in rats possible role of HSP72", 17, pp.520~535 (2001)
- Okuyama A, "Differential induction of HSP60 and HSP72 by different stress situations in rats", 42, pp.1473~1479 (1997)
- Strowski MZ, "Caerulein pancreatitis increases mRNA but reduces protein levels of rat pancreatic heat shock proteins", 273, pp.937~945 (1997)
- Lee H, "Water immersion stress induces heat shock protein 60 expression and protects against pancreatitis in rats", 119, pp.220~229 (2000)
- Dabrowski A, "Cholecystokinin and EGF activate a MAPK cascade by different mechanisms in rat pancreatic acinar cells", 273, pp.1472~1479 (1997)
- Dabrowski A, "Jun kinases are rapidly activated by cholecystokinin in rat pancreas both in vitro and in vivo", 271, pp.5686~5690 (1996)
- Norman J, "The role of cytokines in the pathogenesis of acute pancreatitis", 175, pp.76~83 (1998)
- Lane JS.,, "Platelet activating factor antagonism reduces the systemic inflammatory response in a murine model of acute pancreatitis", 99, pp.365~370 (2001)
- Exley AR, "Endotoxaemia and serum tumour necrosis factor as prognostic markers in severe acute pancreatitis", pp.1126~8 (1992)
- Panozzo MP, "Diagnostic utility of a new monoclonal antibody pancreatic isoamylase assay in chronic pancreatic diseases", pp.485~8 (1990)
- Lampel M, "Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretagogue", pp.97~117 (1977)
- Panozzo MP, "Diagnostic utility of a new monoclonal antibody pancreatic isoamylase assay in chronic pancreatic diseases", pp.485~8 (1990)
- Pagani F, "Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity", bo(ra r), pp.304~8 (1993)
- Pagani F, "Clinical and analytical evaluation of a continuous enzymatic method for measuring pancreatic lipase activity", bo(ra r), pp.304~8 (1993)