본문 바로가기
KERD
논문KCI 등재

황련감초 하태독법의 피부 지방장벽형성 증진효과

Effect of Skin Fat Lipid Barrier Formation on Hataedock with Coptis Japonica & Glycyrrhiza Uralensis

0

조회

0

다운로드

5

인용

0

참고문헌

키워드

주제분류한의학

초록

Objectives This study is conducted to evaluate skin fat barrier formation of Hataedock using the Coptis japonica & Glycyrrhiza uralensis extract. Methods The 3-week-old NC/Nga mice were divided into 3 groups: control group (Ctrl), Hataedock-treated group that uses the Coptis japonica & Glycyrrhiza uralensis (CGT) extract, and Hataedock-treated group that uses Bifidobacterium (BBT). After 2 weeks, changes in immunohistochemicals, and skin-lipid-barrier regulators were observed for the effects of Hataedock. Results In CGT group, loricrin-positive reaction has been increased by 231%, along with involucrin-positive reaction by 90%, filaggrin-positive reaction by 143%, and ASM-positive reaction by 341% in the stratum corneum. Conclusions Hataedock, using the extract of Coptis japonica & Glycyrrhiza uralensis, increased the expression of proteins promoting keratinocyte differentiation. This leads into conclusion that Hataedock may increase the keratinocyte formation and function which promotes skin barrier formation.

Abstract

Objectives This study is conducted to evaluate skin fat barrier formation of Hataedock using the Coptis japonica & Glycyrrhiza uralensis extract. Methods The 3-week-old NC/Nga mice were divided into 3 groups: control group (Ctrl), Hataedock-treated group that uses the Coptis japonica & Glycyrrhiza uralensis (CGT) extract, and Hataedock-treated group that uses Bifidobacterium (BBT). After 2 weeks, changes in immunohistochemicals, and skin-lipid-barrier regulators were observed for the effects of Hataedock. Results In CGT group, loricrin-positive reaction has been increased by 231%, along with involucrin-positive reaction by 90%, filaggrin-positive reaction by 143%, and ASM-positive reaction by 341% in the stratum corneum. Conclusions Hataedock, using the extract of Coptis japonica & Glycyrrhiza uralensis, increased the expression of proteins promoting keratinocyte differentiation. This leads into conclusion that Hataedock may increase the keratinocyte formation and function which promotes skin barrier formation.

참고문헌 (56)

  1. Kim YJ, "The effect of retinoic acid on expression of human beta defensin-2and LL-37 in keratinocyte", J Korean Invest Dermatol, 14(1), pp.29~35 (2007)
  2. 권민수, "세라마이드가 피부장벽 기능에 미치는 효과", 아시안뷰티화장품학술지, 3(1), pp.131~138 (2005), 한국피부과학연구원
  3. 윤영민, "병풀과 엽산을 함유한 복합미생물 발효 추출물 및 아세틸글루타민, 니코틴산 아데닌 디뉴클레오티드 인산 복합물의 피부 세포노화 억제, 콜라겐생성 촉진, 세포재생 및 세포이동 촉진, 항염, 각질세포분화 촉진, 멜라닌생성 억제 효능", 아시안뷰티화장품학술지, 11(4), pp.675~684 (2013), 한국피부과학연구원
  4. Steinert PM, "The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope", J Biol Chem, 270(30), pp.17702~17711 (1995)
  5. Steven AC, "Protein composition of cornified cell envelopes of epidermal keratinocytes", J Cell Sci, 107(2), pp.693~700 (1994)
  6. Elias PM, "Structural and lipid biochemical correlates of the epidermal permeability barrier", Adv Lipid Res, 24, pp.1~26 (1991)
  7. Steinert PM, "Characterization of a class of cationic proteins that specifically interact with intermediate filaments", Proc Natl Acad Sci USA, 78(7), pp.4097~4101 (1981)
  8. Cornelissen C, "IL-31 regulates differentiation and filaggrin expression in human organotypic skin models", J Allergy Clin Immunol, 129(2), pp.426~433 (2012)
  9. Irvine AD, "Breaking the(un)sound barrier : filaggrin is a major gene for atopic dermatitis", J Invest Dermatol, 126(6), pp.1200~1202 (2006)
  10. Palmer CN, "Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis", Nat Genet, 38(4), pp.441~446 (2006)
  11. O'Regan GM, "The role of filaggrin in the atopic diathesis", Clin Exp Allergy, 40(7), pp.965~972 (2010)
  12. Barnes KC, "An update on the genetics of atopic dermatitis : scratching the surface in 2009", J Allergy Clin Immunol, 125(1), pp.16~29 (2010)
  13. Kim BB, "7, 3’, 4’-Trihydroxyisoflavone ameliorates the development of Dermatophagoides farinae-induced atopic dermatitis in NC", Nga mice, 2013, pp.2013, 1~10
  14. Kang MY, "A study on fetal toxicosis removal therapy", J Pediatr Korean Med, 17(1), pp.9~51 (2003)
  15. Tang J, "Berberine and Coptidis rhizoma as novel antineoplastic agents : a review of traditional use and biomedical investigations", J Ethnopharmacol, 126(1), pp.5~17 (2009)
  16. Kaboli PJ, "Targets and mechanisms of berberine, a natural drug with potential to treat cancer with special focus on breast cancer", Eur J Pharmacol, 740, pp.584~595 (2014)
  17. Ortiz LM, "Berberine, an epiphany against cancer", Molecules, 19(8), pp.12349~12367 (2014)
  18. Yu M, "Berberine enhances chemosensitivity to irinotecan in colon cancer via inhibition of NF-κB", Mol Med Rep, 9(1), pp.249~254 (2014)
  19. Chin LW, "Anti-herpes simplex virus effects of berberine from Coptidis rhizoma, a major component of a Chinese herbal medicine, Ching-Wei-San", Arch Virol, 155(12), pp.1933~1941 (2010)
  20. 유영은, "한약재 추출물의 피부 염증 유발 세균에 대한 항균활성", 생명과학회지, 20(7), pp.1143~1150 (2010), 한국생명과학회
  21. Miao KL, "Research progress on the chemical compounds and pharmacology of Sophora flavescens", Nat Prod Res Dev, 13(2), pp.69~73 (2001)
  22. Kusano G, "Pharmaceutical botanical studies on some Glycyrrhiza species", Yakugaku Zasshi, 123(8), pp.619~631 (2003)
  23. 김혜진, "원산지별 감초 추출물의 항균 활성 비교 및 천연방부제로써의 효능 연구", 한국미생물·생명공학회지, 41(3), pp.358~366 (2013), 한국미생물·생명공학회[DOI]
  24. 김혜진, "감초 성분의 항균활성, 정량 및 방부력에 관한 연구", 한국미생물·생명공학회지, 42(4), pp.386~392 (2014), 한국미생물·생명공학회[DOI]
  25. 차호열, "황련-감초 추출물을 이용한 하태독법이 NC", Nga 생쥐에서 유발된 아토피 유사 피부염에 미치는 항염증 효과, 2015, pp.36(4), 486~497[DOI]
  26. Nobaek S, "Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome", Am J Gastroenterol, 95(5), pp.1231~1238 (2000)
  27. Forslind B, "A domain mosaic model of the skin barrier", Acta Derm Venereol, 74(1), pp.1~6 (1994)
  28. Mohsenzadeh M, "Evaluation of antibacterial activity of selected Iranian essential oils against Staphylococcus aureus and Escherichia coli in nutrient broth medium", Pak J Biol Sci, 10(20), pp.3693~3697 (2007)
  29. Rasul A, "Formulation development of a cream containing fennel extract : in vivo evaluation for anti-aging effects", Pharm, 67(1), pp.54~58 (2012)
  30. 유학인, "HaCaT 세포에서 회향 열매의 피부장벽기능과 hyaluronic acid 생성에 미치는 영향", 생명과학회지, 25(8), pp.880~888 (2015), 한국생명과학회
  31. Steinert PM, "The proteins elafin, filaggrin, keratin intermediate filaments, loricrin, and small proline-rich proteins 1 and 2 are isodipeptide cross-linked components of the human epidermal cornified cell envelope", J Biol Chem, 270(30), pp.17702~17711 (1995)
  32. Kwon YB, "Effects of colostrum on keratinocyte differentiation and wound healing", Korean J Invest Dermatol, 14(2), pp.45~50 (2007)
  33. Imokawa G, "Decreased level of ceramides in stratum corneum of atopic dermatitis : an etiologic factor in atopic dry skin?", J Invest Dermatol, 96(4), pp.523~526 (1991)
  34. Choi MJ, "Role of ceramides in barrier function of healthy and diseased skin", Am J Clin Dermatol, 6(4), pp.215~223 (2005)
  35. Hara J, "High-expression of sphingomyelin deacylase is an important determinant of ceramide deficiency leading to barrier disruption in atopic dermatitis", J Invest Dermatol, 115(3), pp.406~413 (2000)
  36. Murata Y, "Abnormal expression of sphingomyelin acylase in atopic dermatitis : an etiologic factor for ceramide deficiency?", J Invest Dermatol, 106(6), pp.1242~1249 (1996)
  37. Fluhr JW, "Generation of free fatty acids from phospholipids regulates stratum corneum acidification and integrity", J Invest Dermatol, 117(1), pp.44~51 (2001)
  38. 송지훈, "두시를 이용한 하태독법의 NC", Nga 생쥐에서 DNFB로 유발된 피부손상 완화 효과, 2016, pp.30(2), 109~115[DOI]
  39. Hachem JP, "pH directly regulates epidermal permeability barrier homeostasis and stratum corneum integrity", cohesion, 2003, pp.121(2), 345~353
  40. Proksch E, "The skin : an indispensable barrier", Exp Dermatol, 17(12), pp.1063~1072 (2008)
  41. Baroni A, "Structure and function of the epidermis related to barrier properties", Clin Dermatol, 30(3), pp.257~362 (2012)
  42. Hachem JP, "Sustained serine proteases activity by prolonged increase in pH leads to degradation of lipid processing enzymes and profound alterations of barrier function and stratum corneum integrity", J Invest Dermatol, 125(3), pp.510~520 (2005)
  43. Hachem JP, "pH directly regulates epidermal permeability barrier homeostasis and stratum corneum integrity", cohesion, 2003, pp.121(2), 345~353
  44. Schmid-Wendtner MH, "The pH of the skin surface and its impact on the barrier function", Skin Pharmacol Physiol, 19(6), pp.296~302 (2006)
  45. Rippke F, "Stratum corneum pH in atopic dermatitis : impact on skin barrier function and colonization with Staphylococcus aureus", Am J Clin Dermatol, 5(4), pp.217~223 (2004)
  46. Elias PM, "The aged epidermal permeability barrier : basis for functional abnormalities", Clin Geriatr Med, 18(1), pp.103~120 (2002)
  47. Steinett PM, "Direct evidence that involucrin is a major early isopeptide cross-linked component of the keratinocyte cornified cell envelope", J Biol Chem, 272(3), pp.2021~2030 (1997)
  48. Kim BE, "Loricrin and involucrin expression is down-regulated by Th2 cytokines through STAT-6", Clin Immunol, 126(3), pp.332~337 (2008)
  49. Smith FJ, "Loss-of-function mutations in the gene encoding filaggrin cause ichthyosis vulgaris", Nat Genet, 38(3), pp.337~342 (2006)
  50. O'Regan GM, "The role of filaggrin in the atopic diathesis", Clin Exp Allergy, 40(7), pp.965~972 (2010)
  51. Pullmannová P, "Effects of sphingomyelin", ceramide ratio on the permeability and microstructure of model stratum corneum lipid membranes, 2014, pp.1838(8), 2115~2126
  52. Jensen JM, "Impaired sphigomyelinase activity and epidermal differentiation in atopic dermatitis", J Invest Dermatol, 122(6), pp.1423~1431 (2004)
  53. Elias PM, "Abnormal skin barrier in the etiopathogenesis of atopic dermatitis", Curr Opin Allergy Clin Immunol, 9(5), pp.437~446 (2009)
  54. Elias PM, ""Outside-to-inside"(and now back to"outside")pathogenic mechanisms in atopic dermatitis", J Invest Dermatol, 128(5), pp.1067~1070 (2008)
  55. Hatano Y, "Interleukin-4 suppresses the enhancement of ceramide synthesis and cutaneous permeability barrier functions induced by tumor necrosis factor-alpha and interferon-gamma in human epidermis", J Invest Dermatol, 124(4), pp.786~792 (2005)
  56. Albanesi C, "IL-4 and IL-13 negatively regulate TNF-alpha-and IFN-gamma-induced beta-defensin expression through STAT-6, suppressor of cytokine signaling(SOCS)-1, and SOCS-3", J Immunol, 179(2), pp.984~992 (2007)

관련 논문AI추천

이 간행물의 다른 논문 (4건)