龍葵 추출물이 백혈병 세포의 Apoptosis 유도에 미치는 영향
Induction of Mitochondria-mediated Apoptosis by Solanum Nigrum in Leukemia Cells
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초록
Objectives In human myeloid leukemia cells, there are no specific features of apoptosis compared with apoptosis in other cell types. Solanum nigrum L.(SNL) is a deciduous tree, which is widely distributed in Korea with reported anti-tumor, anti-inflammatory and non-specific immune-enhancing properties. Although the plant has been clinically used for treating a variety of diseases, its bioactive ingredients are unknown and its mode of action potential has never been investigated. Thus anti-tumor property of methanol extract was investigated. Methods In this study, anti-tumor property of methanol extract was investigated by determining its in vitro growth-inhibitory effects on human myeloid leukemia cells. XTT proliferation assay, DNA fragmentation, immunoblot analysis, densitometric analysis were used. Results 1.The methanol fraction of the extracts of SNL induced mitochondria-mediated apoptosis in human myeloid leukemia cells. 2. The methanol fraction exhibited relatively higher cytotoxic activity in a dose-dependent manner than chloroform, and hexane fraction. 3. Typical ladder profile of Oligonucleosomal fragments were appeared. 4. The secreted cytosolic cytochrome C level was increased by treatment of methanol fraction. Conclusions Methanol fraction of SNL is capable of inducing apoptosis in human myeloid leukemia cells.
Abstract
Objectives In human myeloid leukemia cells, there are no specific features of apoptosis compared with apoptosis in other cell types. Solanum nigrum L.(SNL) is a deciduous tree, which is widely distributed in Korea with reported anti-tumor, anti-inflammatory and non-specific immune-enhancing properties. Although the plant has been clinically used for treating a variety of diseases, its bioactive ingredients are unknown and its mode of action potential has never been investigated. Thus anti-tumor property of methanol extract was investigated. Methods In this study, anti-tumor property of methanol extract was investigated by determining its in vitro growth-inhibitory effects on human myeloid leukemia cells. XTT proliferation assay, DNA fragmentation, immunoblot analysis, densitometric analysis were used. Results 1.The methanol fraction of the extracts of SNL induced mitochondria-mediated apoptosis in human myeloid leukemia cells. 2. The methanol fraction exhibited relatively higher cytotoxic activity in a dose-dependent manner than chloroform, and hexane fraction. 3. Typical ladder profile of Oligonucleosomal fragments were appeared. 4. The secreted cytosolic cytochrome C level was increased by treatment of methanol fraction. Conclusions Methanol fraction of SNL is capable of inducing apoptosis in human myeloid leukemia cells.
참고문헌 (27)
- Antonsson B, "Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways", Mol. Cell. Biochem, 256(1), pp.141~155 (2004)
- Cheung JY, "Polyphyllin D is a potent apoptosis inducer in drug-resistant HepG2 cells", Cancer Lett, 217(2), pp.203~211 (2005)
- 안효섭, "소아과학 제8판", pp.889~894 (2004), (주)대한교과서
- Bennett JM, "Proposed revised criteria for the classification of acute myeloid leukemia", Ann Intern Med, 103(4), pp.626~ (1985)
- 洪性範, "臨床抗癌中草藥", pp.108~110 (1990), 成輔社
- 辛民敎, "臨床本草學", pp.576~577 (1986), 永林社
- 안덕균, "中藥大辭典", pp.4101~4105 (1998), 도서출판정담
- 김미정, "龍葵의 抗腫瘍 效果에 對한 實驗的 硏究", 대한한방부인과학회지, 16(2), pp.98~111 (2003), 대한한방부인과학회
- 서부일, "白朮, 黃芪, 龍葵의 免疫調節作用 및 알레르기 低減化에 關한 硏究", 大韓方劑學會誌, 5(1), pp.184~202 (1997)
- Martinou JC, "Breaking the mitochondrial barrier", Nature Rev, 2(1), pp.63~67 (2001)
- Ghobrial IM, "Targeting apoptosis pathways in cancer therapy", CA Cancer J Clin, 55(3), pp.178~194 (2005)
- Chan SL, "Proteins of the bcl-2 family in apoptosis signalling: from mechanistic insights to therapeutic opportunities", Clin Exp Pharmacol Physiol, 31(3), pp.119~128 (2004)
- Vermes I, "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V", J Immunol Methods, 184(1), pp.39~51 (1995)
- Nagata S, "Apoptosis by death factor", Cell, 88(3), pp.355~365 (1997)
- .Zamzami N, "The mitochondrion in apoptosis:how Pandora’s box open", Nature Rev, 2(1), pp.67~71 (2001)
- Thornberry NA, "Caspases:Enemies within", Science, 281(5381), pp.1312~1316 (1998)
- Steinbach JP, "Apoptosis in gliomas: molecular mechanism and therapeutic implications", J. Neurooncol, 70(2), pp.245~256 (2004)
- Sultana S, "Crude extracts of hepatoprotective plants, Solanum nigrum and Cichorium intybusinhibit free radical-mediated DNA damage", J Ethnopharmacol, 45(3), pp.189~192 (1995)
- Hu K, "Antineoplastic agents III:steroidal glycosides from Solanum nigrum", Planta Med, 65(1), pp.35~38 (1999)
- Prashanth Kumar V, "Cytoprotective role of Solanum nigrum against gentamicin-induced kidney cell(Vero cells) damage in vitro", Fitoterapia, 72(5), pp.481~486 (2001)
- 고유미, "龍葵가 HeLa Cell의 增殖抑制와 apoptosis에 미치는 影響", 대한한방부인과학회지, 18(2), pp.1~11 (2005), 대한한방부인과학회
- Raju K, "Effect of dried fruits of Solanum nigrum LINN against CCl4-induced hepatic damage in rats", Biol Pharm Bull, 26(11), pp.1618~1619 (2003)
- Yen GC, "Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants", Food Chem Toxicol, 39(11), pp.1045~1053 (2001)
- Son young ok, "Cytotoxic and antioxidative effects of glycoprotein isolated from Solanum nigrum L. on MCF-7 cells" (2003), Graduate School of Chonnam National University
- Lee sei jung, "150 kDa glycoprotein isolated solanum nigrum L. kills the HCT-116 (colorectal cancer) cells through activation of apoptotic signals", 학사, pp.2005
- Li J, "Antitumor activity of crude polysaccharides isolated from Solanum nigrum Linne on U14 cervical carcinoma bearing mice", Phytother Res, 21(9), pp.832~840 (2007)
- Lin HM, "Induction of autophagy and apoptosis by the extract of Solanum nigrum Linn in HepG2 cells", J Agric Food Chem, 55(9), pp.3620~3625 (2007)