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황련감초 하태독법과 프로바이오틱스의 대장점막 내 면역조절 효과 비교연구

A Comparative Study of Hataedock versus Probiotics on Immunomodulating Effect in Intestinal Mucosa

김기봉|안상현|차호열
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2017.05|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제31권 제2호|pp. 48-56

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키워드

InflammationProbioticsTh2 skewed conditionHataedockIntestine
주제분류한의학

초록

Objectives Hataedock is a treatment that dispels toxic heat and meconium which has been accumulated to the fetus from a pregnant mother via orally administering herbal extracts to a newborn baby. This study was conducted to compare the efficacy of Hataedock, with using the extract of Coptis japonica & Glycyrrhiza uralensis, to the early administration of probiotics for immunomodulation in the intestinal mucosa. Methods NC/Nga mice were divided into three groups; Control group (no treatment), CGT group (3-week-old mice given the extract of Coptis japonica & Glycyrrhiza uralensis), and MBT group (3-week-old mice given a Bifidobacterium). After 2 weeks, the intestinal mucosa tissues of each group of mice were observed. Immunohistochemical staining for IL-4, IL-13, CD40, FcεRI, p-IκB, EGF, and VEGF in the intestinal mucosa was performed. Results CGT group showed 65% decrease in IL-4, 67% decrease in IL-13, 58% decrease in CD40, 72% decrease in FcɛRI, 76% decrease in p-IκB, 41% increase in EGF and 100% increase in VEGF compared to the control group. MBT group also showed 50% decrease in IL-4, 63% decrease in IL-13, 33% decrease in CD40, 53% decrease in FcɛRI, 46% decrease in p-IκB, 23% increase in EGF and 151% increase in VEGF compared to the control group. Conclusions These results suggest that both Hataedock, with using the extract of Coptis japonica & Glycyrrhiza uralensis, and early administration of probiotics were effective in regulating Th2, relieving inflammation and developing intestinal mucosal tissues. Hataedock with extract of Coptis japonica & Glycyrrhiza uralensis may be more effective for immunomodulation in intestinal mucosa than probiotics.

Abstract

Objectives Hataedock is a treatment that dispels toxic heat and meconium which has been accumulated to the fetus from a pregnant mother via orally administering herbal extracts to a newborn baby. This study was conducted to compare the efficacy of Hataedock, with using the extract of Coptis japonica & Glycyrrhiza uralensis, to the early administration of probiotics for immunomodulation in the intestinal mucosa. Methods NC/Nga mice were divided into three groups; Control group (no treatment), CGT group (3-week-old mice given the extract of Coptis japonica & Glycyrrhiza uralensis), and MBT group (3-week-old mice given a Bifidobacterium). After 2 weeks, the intestinal mucosa tissues of each group of mice were observed. Immunohistochemical staining for IL-4, IL-13, CD40, FcεRI, p-IκB, EGF, and VEGF in the intestinal mucosa was performed. Results CGT group showed 65% decrease in IL-4, 67% decrease in IL-13, 58% decrease in CD40, 72% decrease in FcɛRI, 76% decrease in p-IκB, 41% increase in EGF and 100% increase in VEGF compared to the control group. MBT group also showed 50% decrease in IL-4, 63% decrease in IL-13, 33% decrease in CD40, 53% decrease in FcɛRI, 46% decrease in p-IκB, 23% increase in EGF and 151% increase in VEGF compared to the control group. Conclusions These results suggest that both Hataedock, with using the extract of Coptis japonica & Glycyrrhiza uralensis, and early administration of probiotics were effective in regulating Th2, relieving inflammation and developing intestinal mucosal tissues. Hataedock with extract of Coptis japonica & Glycyrrhiza uralensis may be more effective for immunomodulation in intestinal mucosa than probiotics.

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Th2 skewed condition 조절을 통한 대장점막 내 염증개선효과에 대한 두시 하태독법과 오메가3 비교 연구김기봉, 안상현대한한방소아과학회 · 대한한방소아과학회지2017 · 제31권 제3호황련-감초 추출물을 이용한 하태독법이 NC/Nga 생쥐에서 유발된 아토피 유사 피부염에 미치는 항염증 효과김기봉, 박선영, 안상현, 정아람, 차호열, 천진홍, 최준용대한한방내과학회 · 대한한방내과학회지2015 · 제36권 제4호알레르기성 비염에서 황련-감초 하태독법의 IL-4활성 조절을 통한 항염증효과정아람한의병리학회 · 동의생리병리학회지2019 · 제33권 제2호두시를 이용한 하태독법의 NC/Nga 생쥐에서 DNFB로 유발된 피부손상 완화 효과김기봉, 김호현, 박선영, 송지훈, 안상현, 천진홍한의병리학회 · 동의생리병리학회지2016 · 제30권 제2호하태독법 중 황련감초법이 DNFB로 유발된 NC/Nga 생쥐의 아토피 피부염에 미치는 영향김기봉, 박선영, 안상현, 정아람, 차호열, 천진홍대한한방소아과학회 · 대한한방소아과학회지2015 · 제29권 제4호하태독법의 최신 연구 동향김기봉, 주현주, 천진홍대한한방소아과학회 · 대한한방소아과학회지2020 · 제34권 제4호

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