실외 배양 조건에서 요소를 첨가한 배지 성분에 따른 Spirulina (Arthrospira) platensis의 성장 비교
Comparison of Biomass Production of Spirulina (Arthrospira) platensis in Outdoor Culture Conditions Using Different Media by Urea Addition
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One of the most important challenges facing the Spirulina mass cultivation industry is to find a way to reduce the high production costs involved in production. Although the most commercial medium (Zarrouk’s medium) for Spirulina cultivation is too expensive to use, it contains higher amount of NaHCO3 (16.80 g L−1), trace metals and vitamin solutions. The purpose of this study was to increase the efficiency of Spirulina platensis biomass production by developing a low-cost culture medium at an isolated tropical island such as Chuuk State, Federated States of Micronesia (FSM). This study set out to formulate a lowcost medium for the culture of S. platensis, by substituting nutrients of Zarrouk’s medium using fertilizer grade urea and soil extract with a different concentration of carbon source under natural weather condition. In order to select a low-cost culture medium of S. platensis, 10 culture media were prepared with different concentrations of nitrogen (urea and NaNO3) and NaHCO3. The highest maximum specific growth rate (μmax) and mass production were 0.50 day−1 and 1.05 g L−1 in modified medium (NaHCO3 7.50 g L−1, urea 2.00 g L−1 without NaNO3) among all the synthesized media. Protein (56.14%) and carbohydrate (16.21%) concentrations of the lyophilized standard samples were estimated with highest concentration of glutamic acid (14.93%). This study revealed that the use of a low concentration of urea and NaHCO3 with soil extract was an affordable medium for natural mass cultivation in the FSM.
Abstract
One of the most important challenges facing the Spirulina mass cultivation industry is to find a way to reduce the high production costs involved in production. Although the most commercial medium (Zarrouk’s medium) for Spirulina cultivation is too expensive to use, it contains higher amount of NaHCO3 (16.80 g L−1), trace metals and vitamin solutions. The purpose of this study was to increase the efficiency of Spirulina platensis biomass production by developing a low-cost culture medium at an isolated tropical island such as Chuuk State, Federated States of Micronesia (FSM). This study set out to formulate a lowcost medium for the culture of S. platensis, by substituting nutrients of Zarrouk’s medium using fertilizer grade urea and soil extract with a different concentration of carbon source under natural weather condition. In order to select a low-cost culture medium of S. platensis, 10 culture media were prepared with different concentrations of nitrogen (urea and NaNO3) and NaHCO3. The highest maximum specific growth rate (μmax) and mass production were 0.50 day−1 and 1.05 g L−1 in modified medium (NaHCO3 7.50 g L−1, urea 2.00 g L−1 without NaNO3) among all the synthesized media. Protein (56.14%) and carbohydrate (16.21%) concentrations of the lyophilized standard samples were estimated with highest concentration of glutamic acid (14.93%). This study revealed that the use of a low concentration of urea and NaHCO3 with soil extract was an affordable medium for natural mass cultivation in the FSM.
참고문헌 (36)
- Ahsan MB, "A review on culture, production and use of Spirulina as food for humans and feeds for domestic animals and fish", FAO Fisheries and Aquaculture Circular, 1034, pp.4~7 (2008)
- Ajayan KV, "Enrichment of chlorophyll and phycobiliproteins in Spirulina platensis b y the use of r efl ector l ight a nd nitrogen sources: An in-vitro study", Biomass Bioenergy, 47, pp.436~441 (2012)
- Akao Y, "Enhancement of antitumor natural killer cell activation by orally administered Spirulina extract in mice", Cancer Sci, 100, pp.1494~1501 (2009)
- Alonso DL, "Plants as ‘chemical factories’ for the production of polyunsaturated fatty acids", Biotechnol Adv, 18, pp.481~497 (2000)
- Amha Belay, "The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management", J Am Nutraceut Assoc, 5(2), pp.27~48 (2002)
- Andersen RA, "Algal Culturing Techniques", pp.596~ (2004), ELSEVIER Academic Press
- Avila-Leon I, "Arthrospira platensis biomass with high protein content cultuvated in continuous process using urea as nitrogen source", J Appl Microbiol, 112(6), pp.1086~1094 (2012)
- Ayehunie S, "Inhibition of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis)", J Acquir Immune Defic Syndr Hum Retrovirol, 18, pp.7~12 (1998)
- Becker EW, "Microalgae: Biotechnology and microbiology", pp.293~ (2008), Cambridge University Press
- Celekli A, "Predictive modeling of biomass production by Spirulina platensis as function of nitrate and NaCl concentrations", Bioresource Technol, 100, pp.1847~1851 (2009)
- Costa JAV, "Different nitrogen sources and growth responses of Spirulina platensis in microenvironments", World J Microbiol Biotechnol, 17, pp.439~442 (2001)
- "Cyanotech" (2013)[URL]
- Danesi EDG, "An investigation of effect of replacing nitrate by urea in the growth and production of chlorophyll by Spirulina platensis", Biomass Bioenerg, 23, pp.261~269 (2002)
- Eroarome MA, "Country Pasture", Forage Resource Profiles, 2009, pp.12~
- Faintuch, BL, "Emprego de Algumas Fortes Nitrogenadas na Obtengcao de Biomassa de Oscillatoria limnetica", Revista de Microbologia, 23, pp.32~36 (1992)
- Goksan T, "The growth of Spirulina platensis in different culture systems under greenhouse condition", Turk J Biol, 31, pp.47~52 (2007)
- Hayashi O, "Enhancement of antibody production in mice by dietary Spirulina platensis", J Nutr Sci Vitaminol, 40, pp.431~441 (1994)
- Hong S, "Growth of Spirulina platensis in effluents from wastewater treatment plant of pig farm", J Microbiol Biotechn, 3, pp.19~23 (1993)
- Iwata K, "Effects of Spirulina platensis on plasma lipoprotein lipase activity in fructoseinduced hyperlipidemic rats", J Nutr Sci Vitaminol, 36, pp.165~171 (1990)
- Kay RA, "Microalgae as food and supplement", Crit Rev Food Sci Nutr, 30, pp.555~573 (1991)
- Khan Z, "Nutritional and therapeutic potential of Spirulina", Curr Pharm Biotechnol, 6, pp.373~379 (2005)
- Kulshreshtha A, "Spirulina in health care management", Curr Pharm Biotechnol, 9, pp.400~405 (2008)
- Lun FD, "Culture of Spirulina platensis in human urine for biomass production and O2 evolution", J Zhejiang Univ Sci B, 7, pp.34~37 (2006)
- Mazo VK, "Microalgae Spirulina in human nutrition", Vopr Pitan, 73, pp.45~53 (2004)
- Meeks JC, "Regulation of expression of nitrate and dinitrogen assimilation by Anabaena species", Appl Environ Microbiol, 45, pp.1351~1359 (1983)
- Olguin EJ, "The effect of low light flux and nitrogen deficiency on the chemical composition of Spirulina sp. (Arthrospira) grown on digested pig waste", Bioresource Technol, 77, pp.19~24 (2001)
- Oliveira MACL, "Growth and chemical composition of Spirulina maxima and Spirulina platensis biomass at different temperatures", Aquacult Int, 7, pp.261~275 (1999)
- Pirt SJ, "Principles of microbe and cell cultivation", pp.274~ (1975), John Wiley and Sons
- Raoof BDK, "Formulation of a low-cost medium for mass production of Spirulina", Biomass Bioenergy, 30, pp.537~542 (2006)
- Rasool M, "Appraisal of immunomodulatory potential of Spirulina fusiformis: an in vivo and in vitro study", J Nat Med, 63, pp.169~175 (2009)
- Richmond A, "Handbook of Microalgal Culture", pp.566~ (2004), Blackwell Publishing Company
- Sautier C, "Food value in Spirulina algae in humans", Ann Nutr Aliment, 136, pp.109~120 (1998)
- Schwartz J, "Prevention of e xperimental oral cancer b y extracts of Spirulina- Dunaliella algae", Nutr Cancer, 11, pp.127~134 (1988)
- Thompson RH, "A simplified alternative to the AOAC official method for cholesterol in multicomponent foods", J AOAC Int, 15, pp.236~244 (1993)
- Wu LC, "Antioxidant and antiproliferative activities of Spirulina and Chlorella water extracts", J Agric Food Chem, 53, pp.4207~4712 (2005)
- Zarrouk C, "Contribution à l’étude d’une cyanophycée. Influence de divers’ facteurs physiques et chimiques sur la croissance et la photosynthèse de Spirulina maxima", 박사, pp.1966