본문 바로가기
KERD
논문KCI 등재

절식이 나일 틸라피아 Oreochromis niloticus의 Kiss2, GnRH I mRNA 발현 및 성 스테로이드 호르몬 농도에 미치는 영향

Effects of Fasting on Brain Expression of Kiss2 and GnRH I and Plasma Levels of Sex Steroid Hormones, in Nile Tilapia Oreochromis niloticus

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류해양학

초록

In many fish species, including Nile tilapia (Oreochromis niloticus), gonadal development occurs at the expense of stored energy and nutrients. Therefore, reproductive systems are inhibited by limited food supply. It has been well established that reproductive function is highly sensitive to both metabolic status and energy balance. Nothing is known about the possible mediated connection between energy balance and reproduction. Kisspeptin, a neuropeptide product of the Kiss gene has emerged as an essential gatekeeper of reproduction and may be possibly be linked to energy balance and reproduction in non-mammalians. Thus, in this study, the effect of fasting (10 days) on the expression of kisspeptin and the gonadotropin-releasing hormone (GnRH) gene were assessed in Nile tilapia (male and female) using qRTPCR. In addition, plasma levels of estradiol-17β (E2) and 11-ketotestosterone (11-KT) in adult tilapia were measured by ELISA. In male tilapia, fasting reduced Kiss2 and GnRH I mRNA expression in the brain and 11-KT level in comparison with the fed tilapia (p < 0.05). In females, however, there were no significant differences in GnRH I mRNA expression and E2 between fish subjected to fasting and those fed (p > 0.05). These data indicate the impact of nutritional states on kisspeptin as a potential regulatory mechanism for the control of reproduction in male Nile tilapia.

Abstract

In many fish species, including Nile tilapia (Oreochromis niloticus), gonadal development occurs at the expense of stored energy and nutrients. Therefore, reproductive systems are inhibited by limited food supply. It has been well established that reproductive function is highly sensitive to both metabolic status and energy balance. Nothing is known about the possible mediated connection between energy balance and reproduction. Kisspeptin, a neuropeptide product of the Kiss gene has emerged as an essential gatekeeper of reproduction and may be possibly be linked to energy balance and reproduction in non-mammalians. Thus, in this study, the effect of fasting (10 days) on the expression of kisspeptin and the gonadotropin-releasing hormone (GnRH) gene were assessed in Nile tilapia (male and female) using qRTPCR. In addition, plasma levels of estradiol-17β (E2) and 11-ketotestosterone (11-KT) in adult tilapia were measured by ELISA. In male tilapia, fasting reduced Kiss2 and GnRH I mRNA expression in the brain and 11-KT level in comparison with the fed tilapia (p < 0.05). In females, however, there were no significant differences in GnRH I mRNA expression and E2 between fish subjected to fasting and those fed (p > 0.05). These data indicate the impact of nutritional states on kisspeptin as a potential regulatory mechanism for the control of reproduction in male Nile tilapia.

참고문헌 (47)

  1. Biran J, "Molecular identification and functional characterization of the kisspeptin", kisspeptin receptor system in lower vertebrates, 2008, pp.79, 776~786
  2. Bronson FH., "Food-restricted, prepubertal, female rats : rapid recovery of luteinizing hormone pulsing with excess food, and full recovery of pubertal development with gonadotropin-releasing hormone", Endocrinology, 118, pp.2483~2487 (1986)
  3. Brown RE, "KiSS-1 mRNA in adipose tissue is regulated by sex hormones and food intake", Mol Cell Endocrinol, 281, pp.64~72 (2008)
  4. Burcelin R, "Gonadotropin-releasing hormone secretion from hypothalamic neurones : stimulation by insulin and potentiation by leptin", Endocrinology, 144, pp.4484~4491 (2003)
  5. Cagampang FR, "Involvement of ovarian steroids and endogenous opioids in the fasting-induced suppression of pulsatile LH release in ovariectomized rats", J Endocrinol, 129, pp.321~328 (1991)
  6. Castellano JM, "Change in hypothalamic KiSS-1 system and restoration of pubertal activation of the reproductive axis by kisspeptin in undernutrition", Endocrinology, 146, pp.3917~3925 (2005)
  7. Cowley MA., "Hypothalamic melanocortin neurons integrate signals of energy state", Eur J Pharmacol, 480, pp.3~11 (2003)
  8. Dhillo WS, "The neuroendocrine physiology of kisspeptin in the human", Rev Endocr Metab Dis, 8, pp.41~46 (2007)
  9. Felip A, "Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals", Mol Cell Endocrinol, 312, pp.61~71 (2009)
  10. Filby AL, "The kisspeptin", gonadotropin-releasing hormone pathway and molecular signaling of puberty in fish, 2008, pp.78, 278~289
  11. Forbes S, "Effects of ghrelin on kisspeptin mRNA expression in the hypothalamic medial preoptic area and pulsatile luteinizing hormone secretion in the female rat", Neurosci Lett, 460, pp.143~147 (2009)
  12. Foster DL, "Metabolic interfaces between growth and reproduction. I. Nutritional modulation of gonadotropin, prolactin, and growth hormone secretion in the growth-limited female lamb", Endocrinology, 125, pp.342~350 (1989)
  13. Grone BP, "Food deprivation explains effects of mouthbrooding on ovaries and steroid hormones, but not brain neuropeptide and receptor mRNAs, in an African cichlid fish", Horm Behav, 62, pp.18~26 (2012)
  14. Gruenewald DA, "Reduced gonadotropinreleasing hormone gene expression with fasting in male rat brain", Endocrinology, 132, pp.480~482 (1993)
  15. Horvath TL, "Ghrelin as a potential anti-obesity target", Curr Pharm Design, 9, pp.1383~1395 (2003)
  16. Hoskins LJ, "Interactions between gonadotropin-releasing hormone(GnRH)and orexin in the regulation of feeding and reproduction in goldfish(Carassius auratus)", Horm Behav, 54, pp.379~385 (2008)
  17. Iwasa T, "Sensitivities of mRNA expression levels of Kiss1 and its receptor, Kiss1r, to nutritional status are changed during the developmental period in female rats", J Endocrinol, 207, pp.195~202 (2010)
  18. Kaiya H, "Peptide purification, complementary deoxyribonucleic acid(DNA)and genomic DNA cloning, and functional characterization of ghrelin in rainbow trout", Endocrinology, 144, pp.5215~5226 (2003)
  19. Kaiya H, "Amidated fish ghrelin : purification, cDNA cloning in the Japanese eel and its biological activity", J Endocrinol, 176, pp.415~423 (2003)
  20. Kitahashi T, "Cloning and expression of kiss2 in the zebrafish and medaka", Endocrinology, 150, pp.821~831 (2009)
  21. Kohsaka A, "A significant participation of orexin-A, a potent orexigenic peptide, in the preovulatory luteinizing hormone and prolactin surge in the rat", Brain Res, 898, pp.166~170 (2001)
  22. Lee JH, "KiSS-1, a novel human malignant melanoma metastasis-suppressor gene", J Natl Cancer I, 88, pp.1731~1737 (1996)
  23. Lee YR, "Molecular evolution of multiple forms of kisspeptins and GPR54 receptors in vertebrates", Endocrinology, 150, pp.2837~2846 (2009)
  24. Li S, "Structural and functional multiplicity of the kisspeptin", GPR54 system in goldfish(Carassius auratus), 2009, pp.201, 407~418
  25. Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))method", Methods, 25, pp.402~408 (2001)
  26. Luque RM, "Regulation of hypothalamic expression of KiSS-1 and GPR54 genes by metabolic factors : analyses using models and a cell line", Endocrinology, 148, pp.4601~4611 (2007)
  27. Mechaly AS, "Gene structure analysis of kisspeptin-2(Kiss2)in the Senegalese sole(Solea senegalensis) : characterization of two splice variants of Kiss2, and novel evidence for metabolic regulation of kisspeptin signaling in non-mammalian species", Mol Cell Endocrinol, 339, pp.14~24 (2011)
  28. Messager S, "Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54", P Natl Acad Sci USA, 102, pp.1761~1766 (2005)
  29. Mircea CN, "Metabolic fuel and clinical implications for female reproduction", J Obstet Gynaecol Can, 29, pp.887~902 (2007)
  30. Nakazato M, "A role for ghrelin in the central regulation of feeding", Nature, 409, pp.194~198 (2001)
  31. Nishiguchi R, "Gonadotropin-releasing hormone 2 suppresses food intake in the zebrafish, Danio rerio", Front Endocrinol, 3, pp.122~ (2012)
  32. Parhar IS, "Laser-captured single digoxigenin-labeled neurons of gonadotropin releasing hormone types reveal a novel G protein-coupled receptor(GPR54)during maturation in cichlid fish", Endocrinology, 145, pp.3613~3618 (2004)
  33. Parhar IS, "Ghrelin gene in cichlid fish is modulated by sex and development", Biochem Bioph Res Co, 305, pp.169~175 (2003)
  34. Patterson M, "Administration of kisspeptin-54 into discrete regions of the hypothalamus potently increases plasma luteinising hormone and testosterone in male adult rats", J Neuroendocrinol, 18, pp.349~354 (2006)
  35. Popa SM, "The role of kisspeptins and GPR54 in the neuroendocrine regulation of reproduction", Annu Rev Physiol, 70, pp.213~238 (2008)
  36. Roa J, "KiSS-1 system and reproduction : comparative aspects and roles in the control of female gonadotropic axis in mammals", Gen Comp Endocr, 153, pp.132~140 (2007)
  37. Sakata I, "Ghrelin-producing cells exist as two types of cells, closed-and opened-type cells, in the rat gastrointestinal tract", Peptides, 23, pp.531~536 (2002)
  38. Schneider JE., "Energy balance and reproduction", Physiol Behav, 81, pp.289~317 (2004)
  39. Sisk CL, "Effects of food restriction and restoration on gonadotropin and growth hormone secretion in immature male rats", Biol Reprod, 35, pp.554~561 (1986)
  40. Smith C, "The cost of parental care in Haplochromis argens(Cichlidae)", Environ Biol Fish, 40, pp.99~104 (1994)
  41. Smith C, "The costs of parental care in teleost fishes", Rev Fish Biol Fisher, 5, pp.7~22 (1995)
  42. Tacon P., "Relationships between the expression of maternal behaviour and ovarian development in the mouthbrooding cichlid fish Oreochromis niloticus", Aquaculture, 146, pp.261~275 (1996)
  43. Taranger GL, "Control of puberty in farmed fish", Gen Comp Endocr, 165, pp.483~515 (2010)
  44. Tuziak SM, "Gonadotrophin-releasing hormone in winter flounder(Pseudopleuronectes americanus) : molecular characterization, distribution and effects of fasting", Gen Comp Endocr, 184, pp.9~21 (2013)
  45. Unniappan S, "Goldfish ghrelin : molecular characterization of the complementary deoxyribonucleic acid, partial gene structure and evidence for its stimulatory role in food intake", Endocrinology, 143, pp.4143~4146 (2002)
  46. Volkoff H, "Neuropeptides and the control of food intake in fish", Gen Comp Endocr, 142, pp.3~19 (2005)
  47. Wren AM, "Ghrelin causes hyperphagia and obesity in rats", Diabetes, 50, pp.2540~2547 (2001)

관련 논문AI추천

이 간행물의 다른 논문 (6건)