Pathology Survey of the Manila Clam <i>Ruditapes philippinarum</i> from Hwangdo Tidal Flat in Cheonsu Bay on the West Coast of Korea
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Manila clam Ruditapes philippinarum is present at high rates of density in tidal flats in Cheonsu Bay on the west coast of Korea, where clams often exhibit mass mortalities in late summer. We monitored the pathologic condition of clams at Hwangdo tidal flat (HD) to understand the parasitic impacts on clam fitness. Manila clams were fully ripe in July and spawned during August and September, as the histology indicated. The histology revealed that clams in HD tidal flats were heavily infected by the protozoa parasite Perkinsus olseni, as the monthly prevalence ranged from 53% (September) to 93% (August). In addition, Manila clams were co-infected by the metazoan parasite Cercaria tapetis and Parvatrema duboisi with the prevalence of 0-33% and 0-14%, respectively. Massive hemocyte infiltration and subsequent inflammation were commonly observed from the gills of P. olseni infected clams. Clusters of P. olseni trophozoites and heavy hemocyte infiltration were also observed from the female gonad, suggesting that P. olseni interferes with host gonad maturation. The larval trematode occupied almost the entire host gonad, resulting in gonad castration. In addition, Metacercaria of P. duboisi were observed from the subsurface of the mantle. Ray’s fluid thioglycollate medium assay (RFTM) indicated that clams collected in August and September contained approximately 4.0x106 P. olseni cells/g gills. Condition Index (CI) declined gradually from spring to early summer, and the decline in CI was interpreted as a consequence of the heavy parasitism, as the parasites drain the host’s net energy to be used in somatic growth and gamete production.
Abstract
Manila clam Ruditapes philippinarum is present at high rates of density in tidal flats in Cheonsu Bay on the west coast of Korea, where clams often exhibit mass mortalities in late summer. We monitored the pathologic condition of clams at Hwangdo tidal flat (HD) to understand the parasitic impacts on clam fitness. Manila clams were fully ripe in July and spawned during August and September, as the histology indicated. The histology revealed that clams in HD tidal flats were heavily infected by the protozoa parasite Perkinsus olseni, as the monthly prevalence ranged from 53% (September) to 93% (August). In addition, Manila clams were co-infected by the metazoan parasite Cercaria tapetis and Parvatrema duboisi with the prevalence of 0-33% and 0-14%, respectively. Massive hemocyte infiltration and subsequent inflammation were commonly observed from the gills of P. olseni infected clams. Clusters of P. olseni trophozoites and heavy hemocyte infiltration were also observed from the female gonad, suggesting that P. olseni interferes with host gonad maturation. The larval trematode occupied almost the entire host gonad, resulting in gonad castration. In addition, Metacercaria of P. duboisi were observed from the subsurface of the mantle. Ray’s fluid thioglycollate medium assay (RFTM) indicated that clams collected in August and September contained approximately 4.0x106 P. olseni cells/g gills. Condition Index (CI) declined gradually from spring to early summer, and the decline in CI was interpreted as a consequence of the heavy parasitism, as the parasites drain the host’s net energy to be used in somatic growth and gamete production.
참고문헌 (20)
- Cho YG, "Molecular characterization of Urosporidium tapetis sp. nov., a haplosoridian hyperparasite infecting metacercaria of Parvatrema duboisi (Dollfus 1923), a trematode parasite of Manila clam Ruditapes philippinarum on the west coast of Korea", J Invetebr Pathol, 175(2020), pp.107454~ (2020)
- Choi J K, "Macrobenthos habitat mapping in a tidal flat using remotely sensed data and a GIS-based probabilistic model", Mar Pollut Bull, 62, pp.564~572 (2011)
- Choi KS, "Instantaneous reproductive effort in female American oysters, Crassostrea virginica, measured by a new immunoprecipitation assay", Biol Bull, 186, pp.41~61 (1994)
- Choi KS, "The energetic cost of Perkinsus marinus parasitism in oysters: quantification of the thioglycollate method", J Shellfish Res, 8, pp.125~131 (1989)
- 이혜미, "Are Juvenile Manila Clam Ruditapes philippinarum Free from Perkinsus olseni Infection in Korean Waters?", Ocean Science Journal, 55(4), pp.573~579 (2020), 한국해양과학기술원[DOI]
- Lee HM, "Enhanced growth, gonad maturation, and low-level parasite infection in juvenile Manila clam Ruditapes philippinarum cultured in subtidal cages on the south coast of Korea", Aquaculture, 526, pp.735410~ (2020)[DOI]
- 이혜미, "Negative Impacts of Perkinsus olseni Infection in Manila Clam Ruditapes philippinarum Observed from Tidal Flats in Anmyeondo Island on the West Coast of Korea During Post-Spawning Period", Ocean Science Journal, 56(3), pp.307~316 (2021), 한국해양과학기술원[DOI]
- Nam KW, "High parasite b u rden i ncreases t he s u rfacing a nd mortality of the manila clam (Ruditapes philippinarum)in sandy intertidal mudflats on the west coast of Korea during hot summer", Parasite Vector, 11, pp.42~ (2018)[DOI]
- Park KI, "Spatial distribution of the protozoan parasite, Perkinsus sp., found in the manila clam, Ruditapes philippinarum in Korea", Aquaculture, 203, pp.9~22 (2001)
- Park KI, "Application of Enzyme-Linked Immunosorbent Assay (ELISA) for the study of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia): Ⅱ. Impacts of Perkinsus olseni on clam reproduction", Aquaculture, 251, pp.182~191 (2006)
- Park KI, "Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clams Ruditapes philippinarum", Dis Aquat Org, 66, pp.255~263 (2005)
- Park KI, "Pathology survey of the short-neck clam Ruditapes philippinarum on sandy tidal flats along the coast of Ariake Bay, Kyushu, Japan", J Invertebr Pathol, 99, pp.212~219 (2008)
- 박광재, "서해안 바지락 양식장의 서식환경과 서식밀도의 변화", The Korean Journal of Malacology, 29(3), pp.207~216 (2013), 한국패류학회[DOI]
- Ray SM, "A review of the culture method for detecting Dermocystidium marinum with suggested modifications and precautions", Proc Natl Shellfish Ass, 54, pp.55~69 (1966)
- Uddin MJ, "Seasonal changes in Perkinsus olseni infection and gametogenesis in Manila clam, Ruditapes philippinarum, from Seonjedo Island in Incheon, off the west coast of Korea", J World Aquacult Soc, 41, pp.93~101 (2010)
- Uddin MJ, "Annual reproductive cycle and reproductive efforts of the Manila clam Ruditapes philippinarum in Incheon Bay off the west coast of Korea using a histology-ELISA combined assay", Aquaculture, 364–365, pp.5~32 (2012)
- Villalba A, "Perkinsosis in molluscs: a review", Aquat Living Resour, 17, pp.411~432 (2004)
- Waki T, "Impact of Perkinsus olseni infection on a wild population of Manila clam Ruditapes philippinarum in Ariake Bay, Japan", J Invertebr Pathol, 15, pp.134~144 (2018)
- Yang HS, "Temporal variation of Perkinsus olseni infection intensity in the Manila clam Ruditapes philippinarum in Gomso Bay, off the west coast of Korea", J Shellfish Res, 31, pp.685~690 (2012)
- 양현성, "Pathologic Survey on the Manila Clam Ruditapes philippinarum (Adams and Reeve 1850) from Haeju off the Western Coastal Yellow Sea", Ocean Science Journal, 45(2), pp.93~100 (2010), 한국해양과학기술원