Functional Metagenomic Insights into Antibiotic Resistance Genes from the Uppermost Layer of the East Sea, Korea
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The issue of antibiotic resistance (AR) has become critically important. As the use of antibiotics increases, bacteria develop resistance mechanisms that allow them to survive in antibiotic-contaminated environments. The functional metagenomic approach is more effective than culture-dependent methods, PCR, and sequencing in discovering novel AR genes and resistance mechanisms. In the East Sea, various antibiotic resistance (AR) genes conferring resistance to ampicillin, fosfomycin, polymyxin B and rifampicin were identified. Among these antibiotics, the majority of the cloned resistance genes conferred resistance to ampicillin (55.9%), followed by polymyxin B (23.5%), fosfomycin (11.8%), and rifampicin (8.8%). The host organisms inferred from the annotated AR genes were primarily marine bacterial taxa.
Abstract
The issue of antibiotic resistance (AR) has become critically important. As the use of antibiotics increases, bacteria develop resistance mechanisms that allow them to survive in antibiotic-contaminated environments. The functional metagenomic approach is more effective than culture-dependent methods, PCR, and sequencing in discovering novel AR genes and resistance mechanisms. In the East Sea, various antibiotic resistance (AR) genes conferring resistance to ampicillin, fosfomycin, polymyxin B and rifampicin were identified. Among these antibiotics, the majority of the cloned resistance genes conferred resistance to ampicillin (55.9%), followed by polymyxin B (23.5%), fosfomycin (11.8%), and rifampicin (8.8%). The host organisms inferred from the annotated AR genes were primarily marine bacterial taxa.