딸기 시설재배에서 Arbuscular 균근균의 분포
ution of Arbuscular Mycorrhizal Fungi in Greenhouse Strawberry Plants
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초록
전남 담양과 장흥지역을 중심으로 딸기 시설재배에서 발생하는 내생 균근균 (arbuscular mycorrhizal fungi, AMF)의 분포를 조사하였다. 총 23 농가의 근권 토양을 채취한 후 arbuscular 균근균의 포자를 분리하여 계수한 결과 500㎛ 이상은 0.3개 정도, 355~500㎛는 1.0개 정도, 251~354㎛는 4.2개 정도, 107~250㎛는 50.4개 정도, 45~106㎛는 118개 정도였으며, 토양 30g 당 평균 173.9개 정도의 포자밀도로 arbuscular 균근균이 분포하였다. 딸기 뿌리의 균근 감염 양상을 보면 vesicle 25%, hyphae 4% 등 전체적으로 29% 정도의 감염율을 보였으며, arbuscule은 발견되지 않았다. 분리된 arbuscular 균근균 포자를 수단그라스에 재접종하여 4개월 정도 배양하여 arbuscular 균근균의 동정을 실시한 결과 Glomus sp., Gigaspora sp. 및 Acaulospora sp. 등으로 동정되었다.
Abstract
This study was conducted to investigate into the distribution of arbuscular mycorrhizal fungi (AMF) in the greenhouse soils grown strawberry plants in Damyang and Jangheung districts. Twenty three soil samples were collected from strawberry plants under greenhouse conditions, and mycorrhizal spores in soils were separated using wet-sieving methods. Number of mycorrhizal spores per 30g fresh soil sized over 500㎛, 355~500㎛, 251~354㎛, 107~250㎛ and 45~106㎛ were 0.3, 1.0, 4.2, 50.4 and 119, etc. Total number of spores per 30g fresh soil were 173.9. Root infection by vesicles and hyphae were 25% and 4%, respectively. Mycorrhizal root infection by arbuscules was not shown in strawberry roots. Isolated mycorrhizal spores were inoculated into the host plant of sudangrass to identify the genus of arbuscular mycorrhizal fungi, and propagated for 4 months. As a result of identification, mass propagated mycorrhizal spores were Glomus sp., Gigaspora sp., and Acaulospora sp., and so on.
참고문헌 (11)
- "Inoculation of woody legumes with selected arbuscular mycorrhizal fungi and rhizobia to recover desertified Mediterranean ecosystem", pp.129~133 (1993)
- "Glycine- Glomus-Rhizobium symbiosis effects of mycorrhizae on nodule activity and transpiration in soybeans under drought stress", pp.115~119 (1987)
- "A new method for observing the morphology of vesicular-arbuscular mycorrhizae", pp.2128~2134 (1984)
- "Methods and principles of mycorrhizal research", pp.29~35 (1982)
- "Cell and tissue culture in forestry", 2 (1987)
- "The role of mycorrhizae in plant growth and development Physiology of root-microorganisms associations" (1977)
- "Revised classification of arbuscular mycorrhizal fungi with an emendation of Glomaceae", pp.471~37 491 (1990)
- "Carbon flow in plant microbial associations", pp.473~213 474 (1981)
- "Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection Translocation of the British Mycological Society 55", pp.158~160 (1970)
- "Contribution of ectomycorrhiza to the potential nutrient-absorbing surface of pine", pp.639~644 (1994)
- "Mycorrhizal symbiosis" (1997), Academic Press