산수유 에탄올 추출물의 Propionibacterium acnes에 대한 항균 및 항염활성
Antibacterial and Anti-inflammatory Activity of Corni Fructus Ethanol Extract in Propionibacterium acnes
2
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
Corni fructus, the fruits of Cornus officinalis Sieb. et Zucc. one important ingredient in east asia herbal mixture. This study conducted to evaluate the effects of Corni fructus ethanol extracts on the antibacterial and anti-inflammatory activity by P. acnes. Anti-bacterial activity and cytotoxicity of Corni fructus extracts was analyzed by MTT assay and MIC. The cytokines gene expressions were assesses by real time RT-PCR for IL-1α, TLR-2, COX-2. Activation of NF-κB was observed by immunocytochemistry and confocal microscopy. There were no anti-bacterial effects and cytotoxicity as 15.6 ㎍/ml of Corni fructus ethanol extracts in MTT assay and MIC. Transcription levels of inflammatory cytokines, IL-1α, TLR-2, COX-2 were increased by P. acnes in NHEKs. Corni fructus extracts decreased the up regulated gene transcription levels. Translocation of NF-κB into nucleus by P. acnes was significantly inhibited by Corni fructus extracts. From the results, Corni fructus extracts have inflammatory effects on NHEKs, which decreased the mRNA expressions of IL-1α, TLR-2, COX-2 and inhibit translocation of NF-κB from cytoplasm into nucleus by P. acnes. These results suggests the possibility that anti-bacterial and anti-inflammatory effects of Corni fructus ethanol extracts can be applied to prevention and treatment of inflammatory diseases.
Abstract
Corni fructus, the fruits of Cornus officinalis Sieb. et Zucc. one important ingredient in east asia herbal mixture. This study conducted to evaluate the effects of Corni fructus ethanol extracts on the antibacterial and anti-inflammatory activity by P. acnes. Anti-bacterial activity and cytotoxicity of Corni fructus extracts was analyzed by MTT assay and MIC. The cytokines gene expressions were assesses by real time RT-PCR for IL-1α, TLR-2, COX-2. Activation of NF-κB was observed by immunocytochemistry and confocal microscopy. There were no anti-bacterial effects and cytotoxicity as 15.6 ㎍/ml of Corni fructus ethanol extracts in MTT assay and MIC. Transcription levels of inflammatory cytokines, IL-1α, TLR-2, COX-2 were increased by P. acnes in NHEKs. Corni fructus extracts decreased the up regulated gene transcription levels. Translocation of NF-κB into nucleus by P. acnes was significantly inhibited by Corni fructus extracts. From the results, Corni fructus extracts have inflammatory effects on NHEKs, which decreased the mRNA expressions of IL-1α, TLR-2, COX-2 and inhibit translocation of NF-κB from cytoplasm into nucleus by P. acnes. These results suggests the possibility that anti-bacterial and anti-inflammatory effects of Corni fructus ethanol extracts can be applied to prevention and treatment of inflammatory diseases.
참고문헌 (39)
- Kang SH, "Effects of Lithospermum erythrorhizon on the cytokine gene expression In humankeratinocytes", pp.2010
- 서영배, "Study on the Effects of Corni Fructus about the Anti-allergic Action", 대한본초학회지, 17(1), pp.1~12 (2002), 대한본초학회
- 정진섭, "도해 생약대사전", pp.448~449 (1998), 도서출판 영림사
- Brackett RE, "Effect of various acids on growth and survival Yersinia enterocolitica", J. Food Prot, 50, pp.598~602 (1987)
- 최문희, "동백나무 잎 추출물의 여드름 피부 개선 효과", 아시안뷰티화장품학술지, 10(3), pp.661~672 (2012), 주식회사 한국피부임상과학연구소
- Chung SR, "Toxicity and lectins constituents from the seed of Cornus officinalis", Kor. J. Pharmacogn, 24, pp.177~182 (1993)
- Dejardin E, "Regulation of NF-κB activation by IκB related proteins in adenocarcinoma cells", Oncogene, 18, pp.2567~2577 (1999)
- Dong C, "Map kinases in the immune response", Annu. Rev. Immunol, 20, pp.55~72 (2002)
- Endo T, "Study on the constituents of Cornus Officinalis Siebet Zucc", Yakugaku Zasshi, 93, pp.30~ (1973)
- Graham GM, "Proinflammatory cytokine production by human keratinocytes stimulated with Propionibacterium acnes and P. acnes GroEL", Br. J. Dermatol, 150, pp.421~428 (2004)
- Heller S, "Antipropionibacterial Activity of BAL19403, a Novel Macrolide Antibiotic", Antimicrob Agents Chemother, 51, pp.1956~1961 (2007)
- 전연희, "산수유 에탄올 추출물의 식중독 및 식품 부패 세균에 대한 항균 활성 분석", 동아시아식생활학회지, 22(5), pp.692~700 (2012), 동아시아식생활학회
- Jugeau S, "Induction of toll-like receptors by Propionibacterium acnes", Br. J. Dermatol, 153, pp.1105~1113 (2005)
- 기호균, "여드름 환자에서 세균배양과 항생제 감수성에 관한 연구", 대한피부과학회지, 43(7), pp.871~875 (2005), 대한피부과학회
- 김보람, "물푸레나무 껍질의 항산화 및 항균 활성", 아시안뷰티화장품학술지, 13(3), pp.339~344 (2015), 한국피부과학연구원
- 김창수, "솔잎 추출법에 따른 피부상재균 억제 효능 비교 연구", 아시안뷰티화장품학술지, 10(1), pp.25~30 (2012), 주식회사 한국피부임상과학연구소
- Kim J., "Review of the innate immune response in acne vulgaris: activation of Toll-like receptor 2 in acne triggers inflammatory cytokine response", Dermatology, 211, pp.193~198 (2005)
- 김정은, "여뀌 추출물의 항균 활성과 화장품소재로서의 응용", 한국미생물·생명공학회지, 38(1), pp.112~115 (2010), 한국미생물·생명공학회
- 김나래, "천연물유래 여드름 치료제제의 항균활성 측정", 한국미생물·생명공학회지, 37(1), pp.80~84 (2009), 한국미생물·생명공학회
- 김옥경, "산수유 추출물이 Streptozotocin으로 유발된 흰쥐의 항당뇨 및 항산화 작용에 미치는 효과", 韓國油化學會誌, 22(2), pp.157~167 (2005), 한국유화학회
- 김용두, "산수유의 용매분획별 항균활성", 한국식품영양과학회지, 32(6), pp.829~832 (2003), 한국식품영양과학회
- Kim YE, "Antioxidative effect of ethanol fraction for serveral korean medicinal plant hot water extracts", Kor. J. Food. Nutr, 2, pp.141~144 (1997)
- 권승혁, "산수유 에탄올 추출물의 Sarcoma-180 세포에 대한 항암 효과", 한국식품영양과학회지, 39(7), pp.960~965 (2010), 한국식품영양과학회
- 권승혁, "인체 전립선암세포에서 산수유 에탄올 추출물의 환경호르몬에 대한 방어효과", 한국식품영양과학회지, 38(6), pp.663~666 (2009), 한국식품영양과학회
- 이선숙, "연잎 추출물의 여드름 피부 개선 효과", 아시안뷰티화장품학술지, 10(2), pp.405~413 (2012), 주식회사 한국피부임상과학연구소[DOI]
- 임정민, "산수유 추출물의 compund 48", 80에 의한 비만세포 활성화와 혈관 투과성 억제효과, 2004, pp.17(1), 19~30[DOI]
- Lim YS, "A Study on the MIC of Antibiotics for Propionibacterium acnes in Patients with Acne", Kor. J. Dermatol, 33, pp.437~444 (1995)
- McInturff JE, "The role of toll-like receptors in the pathophysioloy of acne", Semi Cutan Med. Surg, 24, pp.73~79 (2005)
- Nakstsuji T, "Animicrobial Property of Lauric Acid Against Propionibacterium Acnes; Its Therapeutic Potential for Inflammatory Acene Vulgaris", J. Invest Dermatol (2009)
- Oh DH, "Antimicrobial activities of natural medicinal herbs on the food spoilage or foodbone disease microorganisms", Kor. J. Food Sci. Technol, 39, pp.957~963 (1998)
- Park SS, "Growth Inhibition of Propionibacterium acnes by mycelial culture broth of paecilomyces japonica in the mulberry leaf eatract", J. Mushroom Sci. Production, 6, pp.32~37 (2008)
- Park YK, "The antidiabetic effects of extracts from Cornus officinalis seed", Chung-Ang J. Pharm. Sci, 9, pp.5~11 (1995)
- 성준모, "여드름 원인균의 성장에 미치는 오미자와 솔잎의 효과", 한국미생물·생명공학회지, 31(1), pp.69~74 (2003), 한국미생물·생명공학회
- Shnitkind E, "Anti-inflammatory properties of narrow-band blue light", J. Drugs Dermatol, 5, pp.605~610 (2006)
- Toheu E, "Consitituents of Cornus officinalis", Yakugaku Zasshi, 93, pp.30~36 (1973)
- Vowels BR, "Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: implications for chronic inflammatory acne", Infect. Immun, 63, pp.3158~3165 (1995)
- 마충제, "여드름 원인균 Propionibacterium acnes에 대한 생약 추출물의 항균활성 측정", 생약학회지, 42(1), pp.98~101 (2011), 한국생약학회
- 양현옥, "산수유 물추출물이 B16", F10 melanoma세포주의 멜라닌 생성에 미치는 영향, 2002, pp.16(4), 29~822[DOI]
- Zaenglein AL, "Fitzpatrickʹs dermatology in gerneral medicine", pp.690~703 (2007), McGraw-Hill