MALDI-TOF MS 분석법을 통한 클리퍼 모발분진 중 미생물 동정
Identification of Microorganisms from Hair Dust in the Clippers through the MALDI-TOF MS Analysis Method
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목적: 본 연구는 미용업의 위생수준에 관하여 연구하고, 클리퍼의 모발 분진에 오염된 미생물을 신속하게 동정하여 환경위생인식 개선과 소독제 개발의 자료를 제공할 목적으로 수행하였다. 방법: 경기지역 미용실 근무자 248명을 대상으로 설문조사를 하였으며, 50개의 미용실에서 클리퍼 모발 분진을 수거하여 MALDI-TOF 동정법으로 미생물을 동정하였다. 통계처리는SPSSWIN (Ver. 19.0K, SPSS Inc., USA)을 사용하였다. 결과: 미용실내에서의 위생교육은 ‘있다’ 50.4%, 기기 종류별 소독교육은 ‘있다’ 64.9%로 나타났다. 위생교육의 필요성은 ‘대학원이상’ 93.3%로 가장 높게 나타났으며, 손에 피부질환 상태에서계속 근무하는 경우는 51.6%로 나타났다. 클리퍼의 소독은 ‘매일 1회’ 35.9%로 나타났으며, 소독을 하지 않는 이유로는 ‘수시사용의 번거로움’ 35.0%로 가장 높게 나타났다. 클리퍼 분진에서 MALDI-TOF MS 방법으로 동정된 미생물은 Escherichia coli AKD00698이며, score는 208.0으로 나타났다. 결론: 상기 결과를 바탕으로, 미용실 종사자들의 위생관리 인식 교육의필요성을 확인할 수 있었고, 동정된 미생물을 통해 분변 및 다른 균의 오염 가능성을 예측할 수 있어 위생학적으로 매우 중요한 의의를 지닌다.
Abstract
Purpose: This research was conducted to study the hygiene level of beauty shop industry and to provide data for improving the environmental hygiene of beauty shops and developing sanitizers by identifying the contaminated microorganisms from hair dust in the clippers promptly. Methods: This survey targeting 248 beauty shop workers in Gyeonggi province was carried out, and hair dust in the clippers from 50 beauty shops was collected. Additionally, microorganisms were identified by using the MALDI-TOF identification method. The SPSSWIN (Ver. 19.0K, SPSS Inc., USA) was used for statistical processing. Results: For the hygiene education in the beauty shop, 50.4% answered ‘Yes’ and for the sterilization education for each apparatus type, 64.9% answered ‘Yes’. For the necessity for hygiene education, 93.3% of respondents with education level of graduate school graduation or higher answered ‘It is necessary’ and for continuous working with skin disease, 51.6% of the respondents answered ‘Yes’. For clipper sterilization, 35.9% answered ‘Once a day’, and for the reason of no clipper sterilization, 35.0% answered ‘Inconvenience due to frequent usage’. The microorganism identified from the dust in the clipper with the MALDI-TOF MS method was Escherichia coli AKD00698 of which the score was 208.0. Conclusion: The results of this research showed that it is necessary to educate beauty shop workers about the hygiene management. Moreover, it is very important significance in the view of hygiene. Because we are able to predict the contamination possibility of other bacteria.
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