흑삼의 아토피 억제활성에 대한 연구
Study on Anti-atopic Effects of Black Ginseng
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The purpose of this study is to investigate the influence of black ginseng (BG) on induced IL-6, IL-8 and MCP-1 secretion by house dust mite in THP-1 (human acute monocytic leukemia cell line) and EoL-1 (human eosinophilic leukemic cells) cell lines. The house dust mite (Dermatophagoides pteronissinus, D. pteronissinus )plays a key role in the pathogenesis of allergic diseases, including atopic dermatitis and asthma. Monocyte chemoattractant protein 1 (MCP-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) play a pivotal role in mediating the infiltration of various cells into the skin of atopic dermatitis. The korean ginseng (radix and leaf) was steamed to 3 hr at 120℃ under high pressure. The contents of benzopyrene which was known as a tumor promoting agent were analysed. Also, the inhibitory effect of BG on the induced IL-6, IL-8 and MCP-1 secretion by D. pteronissinus extract (DpE) in THP-1 and MCP-1 cells was examined. The BG extract (radix and leaf) potently suppressed the elevated production of IL-6 and IL-8 induced by DpE treatment in THP-1 and EoL-1 cells. Among them, the crude saponin fraction of BG leaf exhibited the most potent inhibitory activity. Based on the present results, it can be suggested that the 3 hr steaming at 120℃ under high pressure is the most optimal processing condition and BG may be useful to develop functional food on atopic dermatitis.
Abstract
The purpose of this study is to investigate the influence of black ginseng (BG) on induced IL-6, IL-8 and MCP-1 secretion by house dust mite in THP-1 (human acute monocytic leukemia cell line) and EoL-1 (human eosinophilic leukemic cells) cell lines. The house dust mite (Dermatophagoides pteronissinus, D. pteronissinus )plays a key role in the pathogenesis of allergic diseases, including atopic dermatitis and asthma. Monocyte chemoattractant protein 1 (MCP-1), interleukin-6 (IL-6) and interleukin-8 (IL-8) play a pivotal role in mediating the infiltration of various cells into the skin of atopic dermatitis. The korean ginseng (radix and leaf) was steamed to 3 hr at 120℃ under high pressure. The contents of benzopyrene which was known as a tumor promoting agent were analysed. Also, the inhibitory effect of BG on the induced IL-6, IL-8 and MCP-1 secretion by D. pteronissinus extract (DpE) in THP-1 and MCP-1 cells was examined. The BG extract (radix and leaf) potently suppressed the elevated production of IL-6 and IL-8 induced by DpE treatment in THP-1 and EoL-1 cells. Among them, the crude saponin fraction of BG leaf exhibited the most potent inhibitory activity. Based on the present results, it can be suggested that the 3 hr steaming at 120℃ under high pressure is the most optimal processing condition and BG may be useful to develop functional food on atopic dermatitis.
참고문헌 (24)
- 은희철, "피부면역학", pp.27~34 (1999), 서울대학교 출판부
- "올리브유 중 벤조피렌잔류허용기준 및 시험법", 식품의약품안전청 고시 제 2008-51호, ~ (2008)
- Chang HK., "Changes of saponin contents in Panax ginseng leaves by different harvesting months", Kor. J. Food Nutr, 11, pp.82~86 (1998)
- Colloff MJ, "Ecposure to house dust mites in homes of people with atopic dermatitis", Br. J. Dermatol, 127, pp.322~327 (1992)
- De Benedetto A, "Atopic dermatitis: a disease caused by innate immune defects?", J. Invest. Dermatol, 129, pp.14~30 (2009)
- Friedmann PS., "The role of dust mite antigen sensitization and atopic dermatitis", Clin. Exp. Allergy, 29, pp.869~872 (2006)
- Han BH, "Degradation of ginseng under mild acidic condition", Planta Med, 44, pp.146~149 (1982)
- Hasegawa H, "Main ginseng saponin metabolites formed by intestinal Prevotella oris in hydrolyzing ginseng saponins", Planta Med, 63, pp.436~440 (1997)
- Ae-Jung Kim, "The Chemopreventive Potential and Anti-inflammatory Activities of Korean Black Ginseng in Colon26-M3.1 Carcinoma Cells and Macrophages", Journal of the Korean Society for Applied Biological Chemistry, 53(1), pp.101~105 (2010), 한국응용생명화학회
- 김효진, "흑삼 제조과정 중 증포 횟수에 따른 에탄올 추출물의 항산화 활성", 한국식품영양과학회지, 40(2), pp.156~162 (2011), 한국식품영양과학회
- Kitagawa I, "Chemical studies of crude drugs (1). Constituents of Ginseng radix rubra", Yakugak Zasshi, 103, pp.612~622 (1983)
- 이지현, "흑삼의 제조 및 항암효과", 동의생리병리학회지, 20(4), pp.951~956 (2006), 대한동의병리학회
- Lee JS, "House dust mite, Dermatophagoides pteronissinus increases expression of MCP-1, IL-6, and IL-8 in human monocytic THP-1 cells", CYTOKINE, 42, pp.365~371, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
- 이미라, "포도주스 침지 제조 흑삼의 Ginsenoside Rg3 함량 변화와 Acetylcholinesterase 억제효과", Journal of Ginseng Research, 33(4), pp.349~354 (2009), 고려인삼학회
- Leung DY., "Atopic dermatitis: new insights and opportunities for therapeutic intervention", J. Allergy Clin. Immunol, 105, pp.860~876 (2000)
- Park JD, "Biological activities and chemistry of saponin from Panax ginseng C. A. Meyer", Phytochem. Rev, 4, pp.159~175 (2005)
- Ring J, "Role of aeroallergens in atopic eczema: Proof of concept with the atopy patch test", J. Am. Acad. Dermatol, 45, pp.49~52 (2001)
- Shimizu Y, "Mite antigen-induced IL-4 and IL-13 production by basophils derived from atopic asthma patients", Clin. Exp. Allergy, 28, pp.497~503 (1998)
- Sohn MH, "Calcium-calmodulin mediates house dust mite-induced ERK activation and IL-8 production in human respiratory epithelial cells", RESPIRATION, 74, pp.447~453
- Suzuki Y, "Enzymatic glycosylation and hydrolysis of ginseng saponins", Proc. 7th Int. Ginseng Symp, pp.373~374 (1998)
- Tan BB, "Doubleblind controlled trial of effect of house dust mite allergen avoidance on atopic dermatitis", Lancet, 347, pp.15~18 (1996)
- Wang CZ, "Saponins composition in american ginseng leaf and berry assayed by high-performance liquid chromatography", J. Agric. Food Chem, 54, pp.2261~2266 (2006)
- Wong CK, "Atopic dermatitis: new insights and opportunities for therapeutic intervention", Int, Immunol, 18, pp.1327~1335 (2006)
- Xie JT, "Antihyperglycemia effects of total ginsenosides from leaves and stem of Panax ginseng", Acta Pharmacol. Sin, 26, pp.1104~1110 (2005)