우슬의 HPLC분석과 20-Hydroxyecdysone 및 25S-Inokosterone의 검량 곡선 비교
HPLC Analysis of Achyranthes fauriei and Comparison of Curves for 20-Hydroxyecdysone and 25S-Inokosterone
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
목적: 본 연구는 기능성 원료로서의 우슬의 생리적 활성 능력과 주요성분의 함량을 분석하여, 우슬의 기능성 원료로서의 가능성을 탐색하고자 하였다. 방법: DPPH 및 ABTS 실험을 통해 라디칼 소거 활성을 분석하였고, 우슬 추출물의 총 폴리페놀 및 총 플라보노이드 함량을 분석하였다. HPLC 분석을 통해 시료 내 각 성분의 농도를 정밀하게 측정하고 정량 분석을 위해 표준물질 20-hydroxyecdysone와 25S-inokosterone를 두 화합물의 검량을 비교 분석하였다. 결과: Achyranthes fauriei 뿌리 추출물의 DPPH 라디칼 제거 활성 실험 결과 AFR-100 E>AFR-50 E>AFR-D.W. 순으로 농도 의존적으로 증가하였다. ABTS 분석결과 AFR-50 E>AFR-100 E>AFR-D.W. 순으로 농도 의존적으로 높아졌다. 총 폴리페놀 함량의 결과는 AFR-100 E>AFR-50 E>AFR-D.W. 순이었으며, 총 플라보노이드 함량 AFR-100 E>AFR-50 E>AFR-D.W. 순이었다. HPLC 분석결과 20-hydroxyecdysone는 AFR-50 E 샘플에서 가장 높은 농도를, 25S-inokosterone는 AFR-100 E 샘플에서 가장 높은 농도를 보였다. 결론: 본 우슬의 in vitro 실험을 통해 20-hydroxyecdysone와 25S-inokosterone 검량분석결과, 두 그래프 모두 상관계수가 매우 높아 농도가 클수록 데이터의 선형 관계가 크며, 플라보노이드 함량이 크게 증가할 수 있음을 확인하였다. 본 연구 결과 우슬의 뿌리가 항산화와 항염 효과가 뛰어난 천연 화합물인 플라보노이드를 다량 포함하고 있음을 확인하였으며, 우슬의 우수한 정량분석 방법을 제시하였다.
Abstract
Purpose: The purpose of this study was to determine the potential of Achyranthes fauriei as a functional ingredient by analyzing its physiological activity and identifying its main components. Methods: Radical scavenging activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. Total polyphenol and flavonoid content in A. fauriei root extracts were measured. The concentrations of each component in the samples were measured by HPLC. For quantitative analysis, the standard substances, 20-hydroxyecdysone and 25S-inokosterone, were used to compare and analyze the calibration of these two compounds. Results: The DPPH radical scavenging activity of Achyranthes fauriei root extracts was increased in the order AFR-100 E>AFR-50 E>AFR-D.W., based on their concentration. The ABTS assay revealed an increase in the order AFR-50 E>AFR-100 E>AFR-D.W., based on concentration. The total polyphenol content was in the order AFR-100 E>AFR-50 E>AFR-D.W.; total flavonoid content followed the same trend. HPLC analysis revealed that 20-hydroxyecdysone had the highest concentration in the AFR-50 E sample, whereas 25S-inokosterone had the highest concentration in the AFR-100 E sample. Conclusion: Based on in vitro studies of the A. fauriei root, a calibration analysis of 20-hydroxyecdysone and 25S-inokosterone revealed very high correlation coefficients, indicating a strong linear relationship as concentration increased, with a significant increase in flavonoid content. The results indicate that A. fauriei root contains a high number of flavonoids, which are natural compounds exhibiting excellent antioxidant and anti-inflammatory effects. We also established a robust quantitative analysis method for A. fauriei.
참고문헌 (14)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Jang GY, "Effects of heat treatment and extraction method on antioxidant activity of several medicinal plants", Journal of the Korean Society of Food Science and Nutrition, 41, pp.914~920 (2012)
- Jung Y, "Changes in the antioxidant activity and rancidity of medicoffee supplemented with Scutellaria baicalensis during storage", Journal of the Korean Society of Food Science and Nutrition, 51, pp.706~712 (2022)
- Kim CS, "The therapeutic effect of Achyranthis Radix on the collagen-induced arthritis in mice", The Korea Journal of Herbology, 25, pp.129~135 (2010)
- Kim DH, "Establishment of content criteria of marker compounds through the monitoring of Achyranthis Radix collected from Korea and China", Analytical Science and Technology, 25, pp.250~256 (2012)
- Kim JS, "Studies on antioxidant and anti-hyperlipidemic effects in induced hypercholesterolemic rats of steamed Achyranthis Bidentatae Radix", The Korea Journal of Herbology, 33, pp.43~60 (2018)
- Kim MS, "Antioxidative and physiological activities of fractions from Pleurospermum kamtschaticumin extracts", Journal of the Korean Society of Food Science and Nutrition, 41, pp.1338~1345 (2012)
- Lee BY, "Antioxidative and antimicrobial activities of Rhodiola rosea root as a cosmetic material", Asian Journal of Beauty and Cosmetology, 20, pp.541~549 (2022)
- Lee DH, "Antioxidant activity and standardization of extraction solvents of SJ004", Journal of Korean Medicine Rehabilitation, 30, pp.67~75 (2020)
- Lee JW, "Isolation of flavonol glycoside related to antioxidant activity from Hippophae rhamnoides leaves", Korean Journal of Medicinal Corp Science, 19, pp.251~256 (2011)
- Lee JA, "Validation of the HPLC method for the standardization of Pentagalloylglucose in Paeonia lactiflora root extract as a functional Ingredient", Asian Journal of Beauty and Cosmetology, 22, pp.103~112 (2024)
- Lee YS, "A comparison of components and biological activities between the hot water extracts of Achyranthes japonica Nakai and Achyranthes bidentata Blume", Journal of Life Science, 24, pp.655~663 (2014)
- UM MS, "The evaluation of anti-oxidation, anti-inflammation and anti-wrinkle activity of Jeju native Achyranthes japonica Naka", Journal of the Korean Applied Science and Technology, 38, pp.1209~1218 (2021)
- Yoo IS, "A systematic review of the functional ingredients and physiological activities of Taraxacum coreanum Nakai", Asian Journal of Beauty and Cosmetology, 21, pp.719~732 (2023)