Antioxidant, Anti-inflammatory and skin-aging Enzymes Inhibition potential of Asystasia vogeliana leaf n-hexane Extract and its Dechlorophyllized Equivalent
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Purpose: The study investigated the antioxidant, anti-inflammatory, photoprotective and skin-aging retardation activities of Asystasia vogeliana leaf n-hexane extract (AVHE) and its dechlorophyllized equivalent (DAVHE). Methods: The total phenolics, flavonoids, vitamin A and vitamin E contents of the extracts were quantified. Antioxidant (DPPH scavenging, ferric reducing antioxidant power, total antioxidant capacity, lipid peroxidation and metal chelating inhibition), anti-inflammatory (lipoxygenase, trypsin and protein denaturation inhibition), photoprotective (SPF and UV blocking) and skin-aging enzymes (collagenase and tyrosinase inhibition) activities were evaluated using standard methods. The components of the most potent extract were identified via GC-MS technique. Results: AVHE and DAVHE possess slight concentrations of total phenolics (9.89±0.21 mg GAE/g and 1.43±0.08 mg GAE/g),and flavonoids (0.68±0.00 mg GAE/g, 2.13±0.00 mg GAE/g); AVHE exhibited significantly higher vitamin A and E content (87.29±0.84 mg RE/g, 12.04±0.11 mgTE/g) than DAVHE (41.29±0.74 mgRE/g, 7.31±0.82 mg TE/g). Both extracts showed moderate antioxidant activity. AVHE demonstrated higher trypsin inhibition (81.44%±0.83), protein denaturation inhibition (62.07%±0.00) and lipoxygenase inhibition (IC50=0.458±0.041 mg/ml). Both extract had similar collagenase and tyrosinase inhibitory activities. AVHE showed a significantly higher sun photoprotective activity (46.35±0.55) than DAVHE (28.92±5.13) and a commercial sunscreen (11.65±0.01). GC-MS analysis identified major constituents in AVHE, including 9,12,15-octadecatrienoic acid, (Z,Z,Z); n-hexadecanoic acid; 1-octadecyne and phytol. Conclusion: A. vogeliana leaf n-hexane extract is a promising candidate for skin care formulation. However, dechlorophyllization slightly reduces its efficacy.
Abstract
Purpose: The study investigated the antioxidant, anti-inflammatory, photoprotective and skin-aging retardation activities of Asystasia vogeliana leaf n-hexane extract (AVHE) and its dechlorophyllized equivalent (DAVHE). Methods: The total phenolics, flavonoids, vitamin A and vitamin E contents of the extracts were quantified. Antioxidant (DPPH scavenging, ferric reducing antioxidant power, total antioxidant capacity, lipid peroxidation and metal chelating inhibition), anti-inflammatory (lipoxygenase, trypsin and protein denaturation inhibition), photoprotective (SPF and UV blocking) and skin-aging enzymes (collagenase and tyrosinase inhibition) activities were evaluated using standard methods. The components of the most potent extract were identified via GC-MS technique. Results: AVHE and DAVHE possess slight concentrations of total phenolics (9.89±0.21 mg GAE/g and 1.43±0.08 mg GAE/g),and flavonoids (0.68±0.00 mg GAE/g, 2.13±0.00 mg GAE/g); AVHE exhibited significantly higher vitamin A and E content (87.29±0.84 mg RE/g, 12.04±0.11 mgTE/g) than DAVHE (41.29±0.74 mgRE/g, 7.31±0.82 mg TE/g). Both extracts showed moderate antioxidant activity. AVHE demonstrated higher trypsin inhibition (81.44%±0.83), protein denaturation inhibition (62.07%±0.00) and lipoxygenase inhibition (IC50=0.458±0.041 mg/ml). Both extract had similar collagenase and tyrosinase inhibitory activities. AVHE showed a significantly higher sun photoprotective activity (46.35±0.55) than DAVHE (28.92±5.13) and a commercial sunscreen (11.65±0.01). GC-MS analysis identified major constituents in AVHE, including 9,12,15-octadecatrienoic acid, (Z,Z,Z); n-hexadecanoic acid; 1-octadecyne and phytol. Conclusion: A. vogeliana leaf n-hexane extract is a promising candidate for skin care formulation. However, dechlorophyllization slightly reduces its efficacy.
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