한우 CCAAT/enhancer-binding protein α (C/EBPα) 유전자의 동정과 mRNA의 발현
Molecular Cloning and mRNA Expression of the Hanwoo CCAAT/enhancer-binding protein α (C/EBPα) gene
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초록
CCAAT/enhancer binding protein(C/EBP)는 지방전구세포의 분화과정에서 발현하는 전사인자군에 속한다. 이러한 C/EBP 중에서 C/EBPα는 지방세포의 분화와 지방침착에 있어 중요한 역할을 수행하는 것으로 알려져 있다. 본 연구에서는 한우 C/EBPα 유전자를 분리 동정하고 그 발현을 조사하였다. 한우 C/EBPα 유전자는 1059bp의 open reading frame으로 구성되어 있으며 353개의 아미노산을 암호화하고 있었다. 그리고 한우 C/EBPα 아미노산을 다른 종과 비교하였을 때 매우 높은 상동성을 나타내었다. Northern blotting 분석에 의하여 12개월 한우에 있어서 C/EBPα mRNA의 분석을 실시한 결과 지방조직에서 가장 높은 발현을 나타내었으며 대장과 폐에서 매우 낮은 발현을 확인할 수 있었다. 또한 12개월, 26개월, 30개월 한우의 등심과 지방에 있어서 C/EBPα 유전자의 발현을 real-time RT-PCR로 분석한 결과 등심에서는 26개월에서 가장 높은 발현을 보였으며 지방에서는 12개월과 26개월에서 높은 발현을 나타내었다.
Abstract
CCAAT/enhancer binding proteins(C/EBP) are a group of transcription factors expressed during preadipocyte differentiation. In the C/EBPs, C/EBPα plays an important role in lipid deposition and adipocyte differentiation. In this studies, we report the identification, characterization, and expression of a Hanwoo C/EBPα. The Hanwoo C/EBPα DNA includes a 1059 bp open reading frame encoding a protein of 353 amino acids. The C/EBPα amino acid sequences of the Hanwoo show strong conservation with the corresponding sequences reported in other species. The distribution of C/EBPα mRNA in various tissues of Hanwoo aged 12 months were investigated using Northern blotting analysis. The highest expression was detected in adipose tissue and more lower expression was detected in colon and lung. We also identified expression of C/EBPα mRNA in Hanwoo sirloin and adipose tissue aged 12, 26, and 30 months by real-time RT-PCR. The higest expression were detected at 26 months in the sirloin and at 12 and 26 months in the adipose tissue.
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