실험적 삼출성 중이염에서 점액섬모 수송 기능에 대한 Nitric Oxide와 Peroxynitrite의 작용

Abstract

Background and Objectives：Nitric oxide (NO)NO is an important mediator in the pathogenesis of otitis media with efusion. The harmful efect of NO is related with NO-derived intermediates such as peroxynitrite (OONO-). The author investigated the role of NO and peroxynitrite on mucociliary activity in experimental otitis media with efusion. Materials and Method：Otitis media with efusion was induced by injecting lipopolysacharide (LPS) transtympanically in guinea pigs. In the NG-nitro-L-arginine methyl ester (L-NAME) group, L-NAME was additionally injected intratympanicaly and intramuscularly. Uric acid (UA) group was treated with intraperi-toneal injection of UA 3 times. After 24 hours, dye transfer time was measured and temporal bones were taken for histopathologic examination. Expresion of peroxynitrite was determined by imunohistochemical stain for 3-nitrotyrosine (3-NT). Results：Transfer time of dye was prolonged in LPS group, whereas it was significantly reduced in L-NAME or UA not statisticaly significant. 3-NT was expresed intensely in subepithelial layer of LPS group, and decreased to mild to moderate degree in the treatment groups. Conclusion：LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite-mediated pathways. This study sugests that inhibition of NO synthesis or scavenging of peroxynitrite may have an adjunctive role in the future treatment of otitis media with efusion. (Korean J Otolaryngol 203 ;46 :720-6)