Aqueous Extract from Fructus Mume Decreases NEUROINFLAMMATION
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Lipopolysaccharide(LPS)-induced neuroinflammation is associated with the pathogenesis of Alzheimer’s disease(AD), which is a key factor underlying the generation of amyloid beta. Requirements for therapies of neurodegenerative diseases such as AD are not only consistent efficacy for long periods, but also the elimination of potential risks for hepatotoxicity. Thus, this study aimed to evaluate the effect of Frunus mume water extract (FME) on LPS-induced hippocampal damage associated with inflammatory signals. ICR male mice(n=30) were divided into three groups. The normal group remained untreated, LPS group (positive control) was stimulated to express LPS-induced hippocampus damage, and FME group(treatment) was stimulated to express LPS-induced hippocampal damage and administered FME at 40 mg/kg/day. Mouse brain tissues were tested by hematoxylin and eosin staining and immunohistochemistry following inflammation markers such as tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase(iNOS), and cyclooxygenase-2(COX-2). FME administered at 40 mg/kg/day for seven days in saline solution significantly reduced brain inflammation in the hippocampi of LPS-stimulated mice. Moreover, FME regulated the expression of inflammatory proteins such as TNF-α, iNOS and COX-2. Our data exhibits that FME attenuates neuroinflammation and damage in brains of LPS-stimulated mice. These findings suggest that FME is a potential therapeutic agent in treating neuroinflammation and recommend further testing on the matter.
Abstract
Lipopolysaccharide(LPS)-induced neuroinflammation is associated with the pathogenesis of Alzheimer’s disease(AD), which is a key factor underlying the generation of amyloid beta. Requirements for therapies of neurodegenerative diseases such as AD are not only consistent efficacy for long periods, but also the elimination of potential risks for hepatotoxicity. Thus, this study aimed to evaluate the effect of Frunus mume water extract (FME) on LPS-induced hippocampal damage associated with inflammatory signals. ICR male mice(n=30) were divided into three groups. The normal group remained untreated, LPS group (positive control) was stimulated to express LPS-induced hippocampus damage, and FME group(treatment) was stimulated to express LPS-induced hippocampal damage and administered FME at 40 mg/kg/day. Mouse brain tissues were tested by hematoxylin and eosin staining and immunohistochemistry following inflammation markers such as tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase(iNOS), and cyclooxygenase-2(COX-2). FME administered at 40 mg/kg/day for seven days in saline solution significantly reduced brain inflammation in the hippocampi of LPS-stimulated mice. Moreover, FME regulated the expression of inflammatory proteins such as TNF-α, iNOS and COX-2. Our data exhibits that FME attenuates neuroinflammation and damage in brains of LPS-stimulated mice. These findings suggest that FME is a potential therapeutic agent in treating neuroinflammation and recommend further testing on the matter.
참고문헌 (13)
- Choonara YE, "Trends in the Molecular Pathogenesis and Clinical Therapeutics of Common Neuro-degenerative Disorders", International Jour-nal of Molecular Sciences, 10, pp.2510~2057 (2009)
- Hardy JA, "Alzheimer's Disease: The Amyloid Cascade Hypothesis", Science, 256, pp.184~185 (1992)
- Pratico D, "Inflammatory Hypotheses: Novel Mechanisms of Alzhei-mer's Neurodegeneration and New Thera-peutic Targets?", Neurobiology Aging, 21, pp.441~445 (2000)
- Lee JW, "Neuro-Inflammation Induced by Lipopolysaccharide Causes Cog-nitive Impairment through Enhancement of Beta-Amyloid Generation", Journal of Neu-roinflammation, 5(1), pp.37~ (2008)
- Liu B, "Role of Microglia in Inflam-mation-Mediated Neurodegenerative Dis-eases: Mechanisms and Strategies for Thera-peutic Intervention", Journal of Pharmacol-ogy Experimental Therapeutics, 304, pp.1~7 (2003)
- McGeer EG, "Inflammatory Pro-cesses in Alzheimer's Disease", Prog Neuro-psychopharmacol Biol Psychiatry, 27, pp.741~749 (2003)
- Ansari N, "Natural Products as Promising Drug Candidates for the Treat-ment of Alzheimer's Disease: Molecular Mechanism Aspect", Current Neuropharma-cology, 11, pp.414~429 (2013)
- Leitzmann C, "Characteristics and Health Ben-efits of Phytochemicals", Forsch Komplement-med, 23(2), pp.69~74 (2016)
- Choi HJ, "Mume Fructus Water Extract Inhibits Pro-Inflammatory Mediators in Lipo-polysaccharide-Stimulated Macro-phages", Journal of Medicinal Food, 10, pp.460~466 (2007)
- Liu L, "The Possible Mechanisms of Fructus Mume Pill in the Treatment of Colitis In-duced by 2 4 6 Trinitrobenzene Sulfonic Acid in Rats", Journal of Ethnopharmacology, 126, pp.557~564 (2009)
- Lee KM, "Fructus Mume Al-leviates Chronic Cerebral Hypoperfusion-In-duced White Matter and Hippocampal Dam-age Via Inhibition of Inflammation and Downregulation of TLR4 and p38 MAPK Sig-naling", BMC Complement Alternative Medi-cine, 15, pp.125~ (2015)
- 이강파, "Protective Effect of Areca catechu Leaf Ethanol Extract Against Ethanol-Induced Gastric Ulcers in ICR Mice", Journal of Medicinal Food, 19(2), pp.127~132 (2016), 한국식품영양과학회[DOI]
- Kim MS, "Fructus Mume Ethanol Extract Prevents Inflammation and Normal-izes the Septohippocampal Cholinergic Sys-tem in a Rat Model of Chronic Cerebral Hy-poperfusion", Journal of Medicinal Food, 19, pp.196~204 (2016)