사람 구강암세포에서 쿼세틴에 의한 항암 및 항전이 효과에 관한 융합연구
Convergence study on anticancer and antimetastasis effect by quercetin in human oral cancer cells
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본 연구는 천연 플라보노이드계 물질인 쿼세틴을 사람 구강암세포주인 HSC-2 세포에 처리한 후 나타나는 항암 및 항전이 효과를 보기 위함이다. 연구 결과를 통하여 쿼세틴은 HSC-2 세포의 세포생존율 과 세포증식율이 감소하였으며, 이 세포 사멸의 경로는 세포 자살 프로그램인 세포자멸사의 경로를 통하여 일어나는 것임을 확인하였다. 또한 세포사멸에 큰 영향을 주지 않았던 농도인 쿼세틴 100 μM 처리군부터 HSC-2 세포의 이동력 과 침습력을 억제하는 효과가 있음을 확인하였다. 따라서 본 연구자는 종합적인 실험 결과를 통하여 구강암 세포에 있어서 200 μM이상의 쿼세틴 처리는 세포자살 프로그램을 가동하여 세포사멸을 유도하고, 100 μM 이상의 쿼세틴 처리는 세포의 이동과 침습을 억제하여 항암, 항전이 활성을 일으키는 것을 밝혔다. 그러므로 쿼세틴은 구강암에 있어 전이를 억제하고 암을 치료하기 위한 항암제로서 충분한 가치가 있음을 시사한다.
Abstract
This study was conducted to investigate the anticancer and antitumor effects of quercetin, which is a natural flavonoid substance in human oral cancer cell line HSC-2 cells. The results of this study showed that quercetin reduses the cell viability and the cell proliferation rate, and it led to the evidences of cell death through apoptosis pathway. Also, lower concetration quercetin over 100 μM were inhibited the cell migration and invasion. In the present study, we conclude that quercetin treatment of more than 200 μM induces apoptosis by activating programed cell death and quercetin treatment of 100 μM or more inhibits the cell migration and invasion rate in oral cancer cells. Therefore, this study suggests that quercetin is of sufficient value as an anticancer drug to inhibit metastasis and to treat cancer.
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