Anti-inflammatory Effects of Chloroform Soluble Fraction from Perilla frutescens Britton Leaves Produced by Radiation Breeding in RAW264.7 Cells
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초록
The present study aimed to determine the anti-inflammatory effects of each solvent fraction of a mutant Perilla frutescens produced by radiation breeding. Following extraction with 80% methanol, P. frutescens was fractionated in the order of hexane, chloroform, ethyl acetate, and butanol; the chloroform fraction exhibited less cytotoxicity, the greatest inhibitory effect on the production of nitric oxide (NO), and the highest rate of inhibition on the generation of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and interferon-β (IFN-β). The chloroform fraction also suppressed the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and reduced the activation of nuclear factor-κB (NF-κB) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Finally, the presence of corosolic acid in the chloroform fraction was identified. Taken together, the present findings indicate that the chloroform fraction obtained from mutant P. frutescens inhibited NO production in LPSstimulated RAW264.7 cells via the suppression of iNOS expression and the inactivation of NF-κB.
Abstract
The present study aimed to determine the anti-inflammatory effects of each solvent fraction of a mutant Perilla frutescens produced by radiation breeding. Following extraction with 80% methanol, P. frutescens was fractionated in the order of hexane, chloroform, ethyl acetate, and butanol; the chloroform fraction exhibited less cytotoxicity, the greatest inhibitory effect on the production of nitric oxide (NO), and the highest rate of inhibition on the generation of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and interferon-β (IFN-β). The chloroform fraction also suppressed the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and reduced the activation of nuclear factor-κB (NF-κB) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Finally, the presence of corosolic acid in the chloroform fraction was identified. Taken together, the present findings indicate that the chloroform fraction obtained from mutant P. frutescens inhibited NO production in LPSstimulated RAW264.7 cells via the suppression of iNOS expression and the inactivation of NF-κB.
참고문헌 (30)
- Boehm U, "Cellular responses to interferon-γ", Ann. Rev. Immunol, 15, pp.749~795 (1997)
- 조병옥, "Protective Effect of Perilla frutescens cv. Chookyoupjaso Mutant Water Extract against Oxidative Injury in vitro and in vivo", Food Science and Biotechnology, 20(6), pp.1705~1711 (2011), 한국식품과학회
- 조병옥, "Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-κB and MAPK Activation", Biomolecules & Therapeutics, 22(4), pp.288~294 (2014), 한국응용약물학회
- Cho W, "Zedoarondiol isolated from the rhizoma of Curcuma heyneana is involved in the inhibition of iNOS, COX-2and pro-inflammatory cytokines via the downregulation of NF-κB pathway in LPS-stimulated murine macrophages", Int. Immunopharmacol, 9, pp.1049~1057 (2009)
- 최원희, "들깨 메탄올 추출물의 acetylcholinesterase 억제활성 및 세포독성 보호효과", 한국식품과학회지, 36(6), pp.1026~1031 (2004), 한국식품과학회
- Coussens LM, "Inflammation and cancer", Nature, 420, pp.860~867 (2002)
- Han DS, "Studies on the cytotoxicity and antitumor activity of Perilla frutescens", Kor. J. Pharm, 25(3), pp.249~257 (1994)
- 한호석, "들깨잎의 품종에 따른 성분분석 및 생리활성 물질 탐색", 한국식생활문화학회지, 19(1), pp.94~105 (2004), 한국식생활문화학회
- Hong C, "Corosolic acid ameliorates atherosclerosis in apolipoprotein E-Deficient mice by regulating the nuclear factor-κB signaling pathway and inhibiting monocyte chemoattractant protein-1 expression", Cir. J, 76, pp.995~1003 (2012)
- Jayakuma KS, "Corosolic acid content and SSR markers in Lagerstroemia specios (L.) pers.: a comparative analysis among populations across the southern western Ghats of India", Phytochemistry, 106, pp.94~103 (2014)
- Jin CH, "Isoegomaketone inhibits lipopolysaccharide-induced nitric oxide production in RAW 264.7macrophages through the heme oxygenase-1 induction and inhibition of the interferon-β-STAT-1 pathway", J. Agric. Food Chem, 58, pp.860~867 (2010)
- Kim JY, "Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages", FEBS Lett, 569, pp.321~326 (2004)
- 류재하, "들깨 잎 추출물의 Nitric Oxide Synthase 저해활성 및Peroxynitrite 소거활성", 생약학회지, 38(2), pp.170~175 (2007), 한국생약학회
- 김미향, "차조기의 에탄올과 물 혼합 추출물의 항균활성", 한국식생활문화학회지, 22(2), pp.266~273 (2007), 한국식생활문화학회
- 김시나, "차조기추출물에 의한 염증성 cytokine 생성억제 및 진통작용에 관한 연구", 동의생리병리학회지, 20(2), pp.414~419 (2006), 한의병리학회
- Kwai T, "TLR signaling", Cell Death Differ, 13, pp.816~825 (2006)
- Lee CB, "Coloured flora of Korea", pp.659~ (1982), Hyang Mun Sa
- Hyun-Ah Lee, "Anti-inflammatory Effect of Perilla frutescens (L.) Britton var. frutescens Extract in LPS-stimulated RAW 264.7 Macrophages", Preventive Nutrition and Food Science, 17(2), pp.109~115 (2012), 한국식품영양과학회[DOI]
- Libby P., "Inflammation and cardiovascular disease mechanisms", Am. J. Clin. Nutr, 83, pp.456S~460S (2006)
- Madoka H, "cDNA isolation and functional expression of myrcene synthase from Perilla frutescens", Biol. Pharm. Bull, 27, pp.1979~1985 (2004)
- Melarejo E, "Monocyte chemoattractant protein-1α key mediator in inflammatory processes", Int. J. Biochem. Cell Biol, 41(5), pp.998~1001 (2009)
- Okuda T, "The components of tannic plants in Japan", Yakugaku zasshi, 106, pp.1108~1111 (1986)
- Park HY, "7,8-Dihydroxyflavone exhibits anti-inflammatory properties by down regulating the NF-κB and MAPK signaling pathway in lipopolysaccharide-treated RAW264.7 cells", Int. J. Mol. Med, 29, pp.1146~1152 (2012)
- Park PH, "KB-34, a newly synthesized chalcone derivative, inhibits lipopolysaccharide-stimulated nitric oxide production in RAW264.7 macrophages via heme oxygenase-1induction and blockade of activator protein-1", Eur. J. Pharmacol, 606(1-3), pp.215~224 (2009)
- Park YD, "Inhibition of an inducible nitric oxide synthase expression by a hexane extract from Perilla frutescens cv. Chookyoupjaso mutant induced by mutagenesis with gamma-ray", J. Radiat. Ind, 3(1), pp.13~18 (2009)
- 소양강, "들깨 잎 추출물에서 분리한 Isoegomaketone(IK)의 항 비만 효능", 생약학회지, 46(4), pp.283~288 (2015), 한국생약학회
- Stuehr D, "Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein", Proc. Natl. Sci, 88, pp.7773~7777 (1991)
- Toshihiro M, "Antidiabetic effects of corosolic acid in KK-Ay diabetic mice", Biol. Pharm. Bull, 29(3), pp.585~587 (2006)
- 우경완, "Triterpenes from Perilla frutescens var. acuta and Their Cytotoxic Activity", Natural Product Sciences, 20(2), pp.71~75 (2014), 한국생약학회
- Yanfeng X, "Corosolic acid induces apoptosis through mitochondrial pathway and caspases activation in human cervix adenocarcinoma HeLa cells", Cancer Lett, 284, pp.229~237 (2009)