감마선 조사가 젖산균의 내산성 및 내담즙성에 미치는 영향
The Effect of Gamma Irradiation on the Acid Tolerance and Bile Tolerance of Lactic Acid Bacteria
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The acid and bile tolerance changes of 5 different lactic acid bacteria (LAB; Lactobacillusparacasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, andLactobacillus debruekii) with gamma irradiation were evaluated. The abilities of LAB to survive inthe acidic conditions at the stomach and the bile acidic conditions at the beginning of the small intestineare the key functions for applying LAB to probiotics. In the results, all of LAB survived morethan 50% after incubation in PBS (pH 2.5) for 2 hr, which indicated more than half of LAB arepossible to pass through the stomach. However, gamma irradiation decreased the acid tolerancesof LAB. The bile tolerances of all bacteria except Lactobacillus acidophilus were observed tosurvive at a 3% oxgall concentration in MRS, and 1 kGy of gamma irradiation to LAB did notaffect any bile tolerances changes. But gamma irradiated Lactobacillus casei and Lactobacilluscasei (3 kGy) showed decreasing survival rate with oxgall added MAS agar. In conclusion, gammairradiation should be applied to yogurt or fermented foods with care because LAB could bechanges their properties on acid and bile tolerances.
Abstract
The acid and bile tolerance changes of 5 different lactic acid bacteria (LAB; Lactobacillusparacasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, andLactobacillus debruekii) with gamma irradiation were evaluated. The abilities of LAB to survive inthe acidic conditions at the stomach and the bile acidic conditions at the beginning of the small intestineare the key functions for applying LAB to probiotics. In the results, all of LAB survived morethan 50% after incubation in PBS (pH 2.5) for 2 hr, which indicated more than half of LAB arepossible to pass through the stomach. However, gamma irradiation decreased the acid tolerancesof LAB. The bile tolerances of all bacteria except Lactobacillus acidophilus were observed tosurvive at a 3% oxgall concentration in MRS, and 1 kGy of gamma irradiation to LAB did notaffect any bile tolerances changes. But gamma irradiated Lactobacillus casei and Lactobacilluscasei (3 kGy) showed decreasing survival rate with oxgall added MAS agar. In conclusion, gammairradiation should be applied to yogurt or fermented foods with care because LAB could bechanges their properties on acid and bile tolerances.
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