감마선 조사가 열처리 겨우살이의 항당뇨 및 세포독성에미치는 영향
Effects of Gamma-irradiation on Anti-diabetic and Cytotoxic Activities of Heat-treated Mistletoe (Viscum album)
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Mistletoe (Viscum album) has been widely used as a functional food material for varioustherapeutic purposes from ancient time. In this study, we examined anti-diabetic and cytotoxicactivities of heated-treated mistletoe and the effects of gamma-irradiation on its activities. Heattreatedmistletoe extract was prepared by heating during different time (3, 6, 9 and 12 h) andgamma-irradiated with different doses of 0, 10, 30, 50, 70 and 100 kGy. Heat-treated mistletoeextracts showed a concentration-dependent cytotoxicity on rat insulinoma RINm5F cells and theeffect was gradually decreased as heating time increased up to 12 h. 12 h heat-treated extract wasno cytotoxic. Gamma-irradiation enhanced the reduction of heat-treated mistletoe-inducedcytotoxicity and the decreasing effect was an irradiating dose-dependent. In particular, all of 70kGy irradiated and heat-treated mistletoe extracts did not showed the cytotoxicity and the effectwas comparable to 12 h heat-treated mistletoe extract. Among those extracts, 3 h heat-treatedmistletoe extract gradually increased the insulin secreting activity by gamma-irradiation and theeffect was the best at 70 kGy, whereas 12 heat-treated extract was no effect. On the test of α-glucosidaseinhibitory activity, 3 h heat-treated mistletoe extract showed the concentration dependenteffects and gamma-irradiation induced more activity at 70 kGy, compared to non-irradiated 3 hand 12 h heated mistletoe extracts. These results suggest that the combination of heat treatmentand gamma-irradiation might be more effective than only heat-treatment for improving the antidiabeticactivity of mistletoe extract and reducing its cytotoxicity.
Abstract
Mistletoe (Viscum album) has been widely used as a functional food material for varioustherapeutic purposes from ancient time. In this study, we examined anti-diabetic and cytotoxicactivities of heated-treated mistletoe and the effects of gamma-irradiation on its activities. Heattreatedmistletoe extract was prepared by heating during different time (3, 6, 9 and 12 h) andgamma-irradiated with different doses of 0, 10, 30, 50, 70 and 100 kGy. Heat-treated mistletoeextracts showed a concentration-dependent cytotoxicity on rat insulinoma RINm5F cells and theeffect was gradually decreased as heating time increased up to 12 h. 12 h heat-treated extract wasno cytotoxic. Gamma-irradiation enhanced the reduction of heat-treated mistletoe-inducedcytotoxicity and the decreasing effect was an irradiating dose-dependent. In particular, all of 70kGy irradiated and heat-treated mistletoe extracts did not showed the cytotoxicity and the effectwas comparable to 12 h heat-treated mistletoe extract. Among those extracts, 3 h heat-treatedmistletoe extract gradually increased the insulin secreting activity by gamma-irradiation and theeffect was the best at 70 kGy, whereas 12 heat-treated extract was no effect. On the test of α-glucosidaseinhibitory activity, 3 h heat-treated mistletoe extract showed the concentration dependenteffects and gamma-irradiation induced more activity at 70 kGy, compared to non-irradiated 3 hand 12 h heated mistletoe extracts. These results suggest that the combination of heat treatmentand gamma-irradiation might be more effective than only heat-treatment for improving the antidiabeticactivity of mistletoe extract and reducing its cytotoxicity.
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