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C57BL/6 마우스에서 편백정유의 모발성장 효과

Hair Growth Effect of Chamaecyparis obtusa Oil in C57BL/6 Mice

김영철|장병수|박영옥
대한미용학회 (The Korean Society for Investigative Cosmetology)|2013.06|대한미용학회지 (Journal of Investigative Cosmetology)|제9권 제2호|pp. 87-95|DOI 10.15810/jic.2013.9.2.002

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키워드

모발성장Hair growthC57BL/6 miceC57BL/6 마우스편백정유형태학적 관찰Chamaecyparis obtusa oilMorphological observation

초록

본 연구는 편백정유의 모발성장 효과를 알아보기 위해 5주령 자성 C57BL/6 마우스의 등 부위에 3% 편백정유를 1일 1회, 주 6회, 매 회 100µl씩 4주간 경피 도포하였다. 실험동물은 정상군 (saline), 양성대조군 (3% minoxidil), 실험군 (3% Chamaecyparis obtusa oil)으로 나누었다. 육안적 관찰은 마우스의 등 부위에 털이 자란 상태를 사진촬영한 후 점수에 따라 판정하였으며, 매주 1회 관찰하였다. 양성대조군과 실험군은 정상군에 비해 각각 83%, 86%의 우수한 모발성장 효과를 보였다. 조직학적 관찰은 피부조직 내 모낭 수, 진피두께, 모낭 깊이의 변화를 보기 위해 hematoxylin and eosin 염색을 통해 관찰하였고, toluidine blue 염색 후 진피 및 피하층 내 비만세포 수를 측정하였다. 실험기간 동안 양성대조군과 실험군은 정상군에 비해 유의하게 (p<0.01) 모낭 수가 많아졌으며, 진피두께는 두꺼워졌다. 모낭 깊이 측정 결과, 실험군은 정상군에 비해 실험 기간 동안 꾸준히 유의하게 증가되었으며 (p<0.05), 비만세포 수는 실험 4주째에 실험군이 정상군에 비해 유의한 (p<0.001) 감소를 보였다.

Abstract

To evaluate the hair growth effect of Chamaecyparis obtusa oil (CO) in C57BL/6 mice, the animals were divided into three groups (fifteen mice each), which consisted of saline (SA) treatment group as the normal group, 3% minoxidil (MXD) treatment group as the positive control group and 3% CO treatment group as the experimental group. One hundred µl of the test solutions were topically applied to the backs of C57BL/6 mice once a day, 6 days a week, for 4 weeks. The backs of the mice were photographed for morphological observation and the hair growth was calculated by a scoring system. The hair growth effect in the MXD and CO groups was observed to be remarkably higher compared to the SA group. In order to observe histological changes, hematoxylin and eosin and toluidine blue stains were used. The results showed that the hair follicle (HF) number and depth and dermal thickness in the MXD and CO groups were significantly (p<0.001) increased, as compared to the SA group, also the number of mast cells was gradually decreased. Taking all the results together, CO showed effects on hair growth in C57BL/6 mice according to morphological and histological observations.

참고문헌 (37)

  1. Ahn JY, "Biological activities of essential oil from Chamaecyparis obtusa", J Soc Cosm Sci Korea, 30, pp.503~507 (2004)
  2. Bienová M, "Androgenetic alopecia and current methods of treatment", Acta Dermatovenerologica Alpina Panonica et Adriatica, 14, pp.5~8 (2005)
  3. Botchkarev VA, "Stress and the hair follicle: exploring the connections", Am J Pathol, 162, pp.709~712 (2003)
  4. Cheng SS, "Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae", Biores Technol, 89, pp.99~102 (2003)
  5. CHOI, INGYU, "Fractions of Chamaecyparis obtusa Display Antiallergic Effect in RBL2H3 Cells", Journal of Microbiology and Biotechnology, 16(11), pp.1747~1752 (2006), 한국미생물·생명공학회
  6. Chrysant SG, "Cardiac changes from beta-blocker, diuretic and minoxidil combination in hypertension", Cardiology, 78, pp.45~52 (1991)
  7. Franzios G, "Insecticidal and genotoxic activities of mint essential oils", J Agric Food Chem, 45, pp.2690~2694 (1997)
  8. Friedman ES, "Allergic contact dermatitis to topical minoxidil solution: etiology and treatment", J Am Acad Dermatol, 46, pp.309~312 (2002)
  9. Germonpré PR, "Characterization of the neurogenic plasma extravasation in the airways", Archives Internationales de Pharmacodynamie et de Thérapie, 329, pp.185~203 (1995)
  10. 곽기섭, "피부사상균에 대한 편백정유의 mono- 및 sesquiterpene 항진균 활성 비교", 목재공학, 34(3), pp.46~55 (2006), 한국목재공학회
  11. Hagemann T, "Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution", Contact Dermatitis, 53, pp.53~55 (2005)
  12. Hordinsky MK, "Medical treatment of noncicatricial alopecia", Sem Cutan Medi Surgery, 25, pp.51~55 (2006)
  13. Imura K, "Influence of TRPV3 mutation on hair growth cycle in mice", Biochem Biophy Res Comm, 363, pp.479~483 (2007)
  14. 전주현, "Larvicidal Activity of Chamaecyparis obtusa and Thuja orientalis Leaf Oils against Two Mosquito Species", Journal of Applied Biological Chemistry, 48(1), pp.26~28 (2005), 한국응용생명화학회
  15. Jung DH, "Composition and antimicrobial activity of the essential oils of Chamaecyparis obtusa in Namhae", 석사, pp.2004
  16. 이문원, "탈모생쥐 모델에서 성장주기별 털의 성장과 탈모와 관련된 인자들의 연구", 대한체질인류학회지, 18(1), pp.13~27 (2005), 대한체질인류학회
  17. Leenen FH, "Mortality after coronary artery occlusion in different models of cardiac hypertrophy in rats", Hypertension, 37, pp.209~215 (2001)
  18. Müller-Röver S, "A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages", J Invest Dermatol, 117, pp.3~15 (2001)
  19. Na KJ, "The sedative effect of stress by essential oils purified from softwoods", Kor J Lab Ani Sci, 14, pp.93~96 (1998)
  20. Nguyen KH, "Pseudo acromegaly induced by the longterm use of minoxidil", J Am Acad Dermatol, 48, pp.962~965 (2003)
  21. Olsen EA, "The importance of dual 5α-reductase inhibition in the treatment of male pattern hair loss: results of a randomized placebo-controlled study of dutasteride versus finasteride", J Am Acad Dermatol, 55, pp.1014~1023 (2006)
  22. 최인규, "편백정유의 항산화활성", 목재공학, 36(6), pp.159~167 (2008), 한국목재공학회
  23. Paul WE, "Lymphokine and cytokine production by FcεRI+ cells", Adv Immunol, 53, pp.1~29 (1993)
  24. Paus R, "The psoriatic epidermal lesion and anagen hair growth may share the same “switch-on” mechanism", Yale J Biol Med, 61, pp.467~476 (1988)
  25. Paus R, "Mast cell involvement in murine hair growth", Develop Biol, 163, pp.230~240 (1994)
  26. Randall VA, "Androgen and human hair growth", Clin Endocr, 40, pp.439~457 (1994)
  27. Randall VA, "Stem cell factor", c-Kit signalling in normal and androgenetic alopecia hair follicles, 2008, pp.197, 11~23
  28. Roh SS, "The hair growth promoting effect of Sophora flavescents extract and its molecular regulation", J Dermatol Sci, 30, pp.43~49 (2002)
  29. Roy RK, "Hair growth promoting activity of Eclipta alba in male albino rats", Arch Dermatol Res, 300, pp.357~364 (2008)
  30. Springer K, "Common hair loss disorders", J Am Fam Phys, 68, pp.93~102 (2003)
  31. Stenn KS, "Controls of hair follicle cycling", Physiol Rev, 81, pp.449~494 (2001)
  32. Stough D, "Psychological effect, pathophysiology, and management of androgenetic alopecia in men", Mayo Clinic Proceed, 80, pp.1316~1322 (2005)
  33. Tobin DJ, "Do hair bulb melanocytes undergo apoptosis during hair follicle regression (catagen)?", J Invest Dermatol, 111, pp.941~947 (1998)
  34. Tsujimoto H, "Evaluation of the permeability of hair growing ingredient encapsulated PLGA nanospheres to hair follicles and their hair growing effects", Bioorg Med Chem Lett, 17, pp.4771~4777 (2007)
  35. Tu JB, "Effect of ethosomal minoxidil on dermal delivery and hair cycle of C57BL", 6 mice, 2007, pp.45, 135~137
  36. van der Velden J, "Minoxidil-induced cardiac hypertrophy in guinea pigs", Cellul Mol Life Sci, 55, pp.788~798 (1999)
  37. Yang JK, "Chemical composition and antimicrobial activity of Chamaecyparis obtusa leaf essential oil", Fitoterapia, 78, pp.149~152 (2007)

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