흰 모발과 흑갈색 모발의 영구염모제 염색 전·후 투과전자현미경 관찰
Transmission Electron Microscopy of Gray and Black-brown Hairs Before and After Permanent Hair Dyeing
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The current study examined microstructural changes in gray and black-brown hairs before and after permanent hair dyeing using transmission electron microscopy (TEM). A close connection of cuticle cells to the cell membrane complex (CMC) was observed on TEM images of black-brown and gray hairs. A cuticle cell layer comprising the exocuticle and endocuticle was easily distinguished due to the difference in electron density. The cuticle CMC was also composed of two inner and outer β layers with low electron density and one δ layer with high electron density. The cortex was distinguished from the cuticle layer by cuticle CMC and the medulla was distinguished from the cortex. Holes varying in size were observed in the endocuticle of black-brown hair, however their presence in the gray hair was unlikely. The cortical cells of black-brown hair were filled with macrofibrils, melanin granules, and cell debris, with the presence of melanin granules in the intermacrofibrilar matrix and cell debris. The cortex of the gray hair was filled with macrofibrils, and no melanin granules were observed. Dyeing reaction materials were observed as particles with high electron density in the endocuticles, cuticle and cortex CMCs, intermacrofibrilar matrix, and cellular debris in dyed black-brown hair and dyed gray hair. Characteristically, an increase in the thickness of both cuticle and cortex CMCs was observed after dyeing (0.04 µm) compared with that before dyeing (0.02 µm). In addition, in cuticle CMC, the outer β layer was strikingly thickened compared with the inner β layer.
Abstract
The current study examined microstructural changes in gray and black-brown hairs before and after permanent hair dyeing using transmission electron microscopy (TEM). A close connection of cuticle cells to the cell membrane complex (CMC) was observed on TEM images of black-brown and gray hairs. A cuticle cell layer comprising the exocuticle and endocuticle was easily distinguished due to the difference in electron density. The cuticle CMC was also composed of two inner and outer β layers with low electron density and one δ layer with high electron density. The cortex was distinguished from the cuticle layer by cuticle CMC and the medulla was distinguished from the cortex. Holes varying in size were observed in the endocuticle of black-brown hair, however their presence in the gray hair was unlikely. The cortical cells of black-brown hair were filled with macrofibrils, melanin granules, and cell debris, with the presence of melanin granules in the intermacrofibrilar matrix and cell debris. The cortex of the gray hair was filled with macrofibrils, and no melanin granules were observed. Dyeing reaction materials were observed as particles with high electron density in the endocuticles, cuticle and cortex CMCs, intermacrofibrilar matrix, and cellular debris in dyed black-brown hair and dyed gray hair. Characteristically, an increase in the thickness of both cuticle and cortex CMCs was observed after dyeing (0.04 µm) compared with that before dyeing (0.02 µm). In addition, in cuticle CMC, the outer β layer was strikingly thickened compared with the inner β layer.
참고문헌 (14)
- Imai T, "The influence of hair bleach on the ultrastructure of human hair with special reference to hair damage", Okajimas Folia Anat Jpn, 88, pp.1~9 (2011)
- Inoue T, "Structural analysis of human hair cuticle using microbeam X-ray diffraction : relationship with effects of hair dyeing", J Cosmet Sci, 58, pp.11~17 (2007)
- 이귀영, "파주에서 발견된 16세기 미라 머리카락의 미세구조과보존상태에 관한 연구", 한국현미경학회지, 35(4), pp.211~218 (2005), 한국현미경학회
- 이종순, "두피 모발의 굵기에 따른 큐티클층의 미세구조적 특성", 대한미용학회지, 11(1), pp.81~87 (2015), 대한미용학회[DOI]
- 이수비, "영구염모제로 처리한 흰 모발과 흑갈색 모발의 구성 원소성분 변화", 대한미용학회지, 16(2), pp.101~106 (2020), 대한미용학회[DOI]
- 이수비, "흰 모발과 흑갈색 모발의 영구염모제 염색 전·후 미세구조 변화", 대한미용학회지, 17(2), pp.127~136 (2021), 대한미용학회[DOI]
- Richena M, "Morphological degradation of human hair cuticle due to simulated sunlight irradiation and washing", J Photochem Photobiol B, 161, pp.430~440 (2016)
- Scanavez C, "Extractable substances from human hair : a discussion about the origin of the holes", Colloids Surf B Biointerfaces, 39, pp.39~43 (2004)
- Scanavez C, "Human hair: color changes caused by daily care damages on ultra-structure", Colloids Surf B Biointerfaces, 28, pp.39~52 (2003)
- Seshadri IP, "In situ tensile deformation charactrerization of human hair with atomic force microscopy", Acta Mater, 56, pp.774~781 (2008)
- Slominski A, "Hair follicle pigmentation", J Invest Dermatol, 124, pp.13~21 (2005)
- Swift JA, "The chemistry of human hair cuticle-I : a new method for the physical isolation of cuticle", J Soc Cosmet Chem, 25, pp.13~22 (1974)
- Wolfram LJ, "Human hair: a unique physicochemical composite", J Am Acad Dermatol, 48, pp.S106~S114 (2003)
- Yang FC, "The structure of people’s hair", Peer J, 2, pp.e619~ (2014)