목련 잎 추출물의 생리 활성 및 PCL-PEG 고분자 미셀과 세포투과 펩티드를 적용한 피부흡수증진 효과
Physiological Activity of Magnolia kobus A. P. DC. Leaf Extracts and Enhancement of Skin Permeation Using PCL-PEG Polymer Micelle and Cell Penetrating Peptide
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In this study, we report the development of a cosmetic material from Magnolia kobus A. P. DC. leaves. Following an assessment of the anti-oxidant, anti-wrinkle, and whitening effects of the hydrothermal and ethanol extracts, total polyphenol contents were quantified as 39.68 mg/g and 60.95 mg/g respectively, while the DPPH radical scavenging activity (IC50 values) were 476.03 μg/mL and 161 μg/mL respectively, and the SOD-like activities were 79.80% and 97.75%, respectively at a concentration of 100 μg/mL. At a concentration of 1,000 μg/mL, the elastase inhibition activities of hydrothermal and ethanol extracts were 55.17% and 57.77% respectively, and at concentration of 4,000 μg/mL, the tyrosinase inhibition activities were 27.36% and 75.58%, respectively. To solve the problem of insolubility with ethanol extract, which is more physiologically active than hydrothermal extract, PCL-PEG polymeric micelle was used resulting in solubility of the ethanol extract and reductions in particle sizes of greater than one-tenth. In addition, to improve skin permeability, 0.1% cell-penetrating peptide R6 (hexa-D-arginine) was used; the cumulative permeated amounts of: i) 1.0% ethanol extract, ii) ethanol extract and distilled water (1:1 mixture solvent), and iii) PCL-PEG polymeric micelle containing 1.0% ethanol extract, were 58.07 μg/cm², 75.86 μg/cm², 95.59 μg/cm², respectively. In other words: i) various physiological activities were affected by ethanol extracts, ii) the ethanol extract was solubilized by PCL-PEG and, iii) skin absorption was increased following addition of the cell-penetrating peptide, R6. These results confirm the potential for Magnolia kobus A. P. DC. leaf ethanol extract to serve as a new, natural functional cosmetic material.
Abstract
In this study, we report the development of a cosmetic material from Magnolia kobus A. P. DC. leaves. Following an assessment of the anti-oxidant, anti-wrinkle, and whitening effects of the hydrothermal and ethanol extracts, total polyphenol contents were quantified as 39.68 mg/g and 60.95 mg/g respectively, while the DPPH radical scavenging activity (IC50 values) were 476.03 μg/mL and 161 μg/mL respectively, and the SOD-like activities were 79.80% and 97.75%, respectively at a concentration of 100 μg/mL. At a concentration of 1,000 μg/mL, the elastase inhibition activities of hydrothermal and ethanol extracts were 55.17% and 57.77% respectively, and at concentration of 4,000 μg/mL, the tyrosinase inhibition activities were 27.36% and 75.58%, respectively. To solve the problem of insolubility with ethanol extract, which is more physiologically active than hydrothermal extract, PCL-PEG polymeric micelle was used resulting in solubility of the ethanol extract and reductions in particle sizes of greater than one-tenth. In addition, to improve skin permeability, 0.1% cell-penetrating peptide R6 (hexa-D-arginine) was used; the cumulative permeated amounts of: i) 1.0% ethanol extract, ii) ethanol extract and distilled water (1:1 mixture solvent), and iii) PCL-PEG polymeric micelle containing 1.0% ethanol extract, were 58.07 μg/cm², 75.86 μg/cm², 95.59 μg/cm², respectively. In other words: i) various physiological activities were affected by ethanol extracts, ii) the ethanol extract was solubilized by PCL-PEG and, iii) skin absorption was increased following addition of the cell-penetrating peptide, R6. These results confirm the potential for Magnolia kobus A. P. DC. leaf ethanol extract to serve as a new, natural functional cosmetic material.
참고문헌 (21)
- 안나영, "Streptozotocin에 의해 유도된 당뇨모델동물에서 황칠나무 (Dendropanax morbifera Leveille)의 열수추출물과 에탄올추출물의 당뇨 질환 개선 효능", Journal of Nutrition and Health, 47(6), pp.394~402 (2014), 한국영양학회
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 81, pp.1199~1200 (1958)
- 최미현, "끄라차이담 발효물의 항산화 활성과 Tyrosinase 및 Elastase 저해활성", 한국식품영양과학회지, 47(11), pp.1076~1084 (2018), 한국식품영양과학회
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- 현훈, "선형 및 분지 구조의 폴리(에틸렌 글리콜)", 폴리카프로락톤 공중합체의 합성 및 특성 검토, 2006, pp.30(2), 146~151[DOI]
- 김은남, "Franz Diffusion Cell을 이용한 인진호 함유 경피제의 피부투과 특성 연구", 생약학회지, 49(2), pp.165~171 (2018), 한국생약학회
- 박수남, "Vitamin E Acetate를 함유한 Nano-emulsion 제조와 Franz Diffusion Cell을 이용한 Vitamin E Acetate의 경표피 흡수에 관한 In Vitro 연구", 대한화장품학회지, 35(2), pp.91~101 (2009), 사단법인 대한화장품학회
- 김정옥, "상황버섯 열수 및 에탄올 추출물의 항산화 효과 및 생리활성", 한국식품영양과학회지, 37(6), pp.684~690 (2008), 한국식품영양과학회
- 김시경, "국산 홍합과 뉴질랜드 초록입 홍합 열수 추출물의 알코올분해효소 활성에 미치는 영향 및 DPPH 라디칼 소거능과 Angiotensin Converting Enzyme 저해 활성", 한국식품영양과학회지, 43(9), pp.1363~1368 (2014), 한국식품영양과학회
- Kubo I, "Tyrosinase inhibitors from Anacardium occidentale fruits", J Nat Prod, 57, pp.545~551 (1994)
- 곽연주, "부추의 건조 온도 조건별 클로로필, Superoxide Dismutase 유사활성의 변화", 한국식품영양과학회지, 38(7), pp.879~884 (2009), 한국식품영양과학회
- 이병근, "화장품 개발을 위한 배롱나무(Lagerstroemia indica Linnaeus) 가지 추출물의 생리활성에 관한 연구", 한국자원식물학회지, 27(1), pp.29~34 (2014), 한국자원식물학회[DOI]
- 이소희, "품종별 렌틸 추출물의 폴리페놀화합물 함량 및 항산화 활성", 한국식품영양과학회지, 45(7), pp.973~979 (2016), 한국식품영양과학회
- 이성숙, "목련과 식물의 항균 및 항산화활성", 목재공학, 38(6), pp.579~586 (2010), 한국목재공학회
- 이양숙, "가죽나무(Ailanthus altissima) 물 추출물의 항산화 활성과 Tyrosinase 저해", 한국식품영양과학회지, 36(9), pp.1113~1119 (2007), 한국식품영양과학회
- 임애경, "포공영의 열수 및 에탄올 추출물의 기능적 생리활성", 한국식품영양과학회지, 37(10), pp.1231~1237 (2008), 한국식품영양과학회
- 임규남, "Quercetin과 Rutin을 함유하는 PCL-b-PEG 고분자 미셀의 특성 및 피부 흡수에 관한 In vitro 연구", 폴리머, 36(4), pp.420~426 (2012), 한국고분자학회[DOI]
- Lundberg P, "A brief introduction to cell-penetrating peptides", J Mol Recognit, 16, pp.227~233 (2003)
- 박찬익, "하엽(荷葉) 추출물이 항산화 효능 및 피부노화에 미치는 영향", 대한본초학회지, 31(4), pp.53~60 (2016), 대한본초학회
- Wender PA, "The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake : peptoid molecular transporters", PNAS, 97, pp.13003~13008 (2000)
- Yoo ID, "Development of new natural antioxidants for cosmeceuticals", J Soc Cosmet Scientists Korea, 31, pp.349~357 (2005)