사람 구강암세포 KB에서 bavachin에 의한 세포성장 억제
Cell growth inhibition by bavachin in KB human oral cancer cells
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Psoralea corylifolia is widely distributed over southeastern Asian countries and has been used in traditional Chinese medicine. Bavachin, one of the main constituents of P. corylifolia, possesses various biological activities such as inhibition of platelet aggregation, anti-oxidation, and anti-bacterial activity. However, the anti-cancer activity of bavachin has not yet been well defined. In this study, the cytotoxic activity of bavachin and its mechanism of cell death were examined in KB human oral cancer cells. Treatment of KB cells with bavachin cells induced inhibition of cell growth in a concentration- and a time-dependent manner as determined by MTT assay. Treatment with bavachin induced nuclear condensation and DNA fragmentation in KB cells. Proteolytic processing of caspases-3, -7, -8, and -9 was elevated by bavachin treatment in KB cells. These results suggest that bavachin can induce suppression of cell growth and cell apoptosis in KB human oral cancer cells, and thus it may have potential effects for anti-cancer drug discovery.
Abstract
Psoralea corylifolia is widely distributed over southeastern Asian countries and has been used in traditional Chinese medicine. Bavachin, one of the main constituents of P. corylifolia, possesses various biological activities such as inhibition of platelet aggregation, anti-oxidation, and anti-bacterial activity. However, the anti-cancer activity of bavachin has not yet been well defined. In this study, the cytotoxic activity of bavachin and its mechanism of cell death were examined in KB human oral cancer cells. Treatment of KB cells with bavachin cells induced inhibition of cell growth in a concentration- and a time-dependent manner as determined by MTT assay. Treatment with bavachin induced nuclear condensation and DNA fragmentation in KB cells. Proteolytic processing of caspases-3, -7, -8, and -9 was elevated by bavachin treatment in KB cells. These results suggest that bavachin can induce suppression of cell growth and cell apoptosis in KB human oral cancer cells, and thus it may have potential effects for anti-cancer drug discovery.
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