Bone regeneration effect of lidocaine-maltodextrin-thrombin collagen on rat calvarial bone defect model
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초록
We evaluated the effects of lidocaine–maltodextrin–thrombin (LMT) collagen on new bone formation on a rat calvarial defect model. Thirty-six rats were randomly divided into three groups. A single bone window was prepared in the middle of the calvarium, and each bone defect was treated separately. In Group 1, the bone defect was sutured without any treatment. In group 2, only the collagen plug was inserted into the bone defect, and in group 3, the collagen plug soaked with LMT collagen was inserted. After four weeks, six animals were sacrificed randomly in each group, and samples of the skull area were then collected and subjected to micro-computed tomography and hematoxylin and eosin and Masson’s trichrome staining. At eight weeks postsurgery, the remaining six rats in each group were treated identically. Consequently, no significant differences were noted in the bone formation between the groups at week 4. At week 8, a significant increase in new bone formation was observed in rats treated with collagen plug plus LMT collagen compared with untreated rats and rats treated only with the collagen plug. Therefore, the combination of LMT collagen and collagen plug is expected to function as an effective scaffold, which can provide clinicians with various possibilities and options for repairing bone defects.
Abstract
We evaluated the effects of lidocaine–maltodextrin–thrombin (LMT) collagen on new bone formation on a rat calvarial defect model. Thirty-six rats were randomly divided into three groups. A single bone window was prepared in the middle of the calvarium, and each bone defect was treated separately. In Group 1, the bone defect was sutured without any treatment. In group 2, only the collagen plug was inserted into the bone defect, and in group 3, the collagen plug soaked with LMT collagen was inserted. After four weeks, six animals were sacrificed randomly in each group, and samples of the skull area were then collected and subjected to micro-computed tomography and hematoxylin and eosin and Masson’s trichrome staining. At eight weeks postsurgery, the remaining six rats in each group were treated identically. Consequently, no significant differences were noted in the bone formation between the groups at week 4. At week 8, a significant increase in new bone formation was observed in rats treated with collagen plug plus LMT collagen compared with untreated rats and rats treated only with the collagen plug. Therefore, the combination of LMT collagen and collagen plug is expected to function as an effective scaffold, which can provide clinicians with various possibilities and options for repairing bone defects.
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