Physiological Responses of Paddy Weeds to Bensulfuron-methyl Herbicide
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This study was conducted to compare the susceptibility among weed species to bensulfuron-methyl, ALS (acetolactate synthase) activities and to determine the several physiological characteristics in the treated plants. Chlorophyll contents in Oryza sativa, Ludwigia prostrata, and Bidens tripartita were increased as the concentration of bensulfuron-methyl increased to 10 μM in media. A notable increase in the reducing sugar content was observed in Aneilema keisak and L. prostrata. Effect of bensufuron-methyl treatment on soluble protein content was different in tested plants. The free amino acid contents in L. prostrata and B. tripartita which are susceptible to this herbicide was significantly increased as the bensulfuron-methyl concentration was increased, however, there was no clear relationship between the free amino acid content and the degree of resistance to sulfonylurea herbicide. The acetolactate synthase activity measured in vitro was decreased drastically as bensulfuron- methyl concentration increased. The I50 values by bensulfuron -methyl for ALS from four tested plant species ranged from 28 nM to 39 nM. Our results of response spectrum and physiological response to sulfonylurea herbicide in Korean weeds may give some clues to set up the appropriate practical schedule for weed control in direct seeding field.
Abstract
This study was conducted to compare the susceptibility among weed species to bensulfuron-methyl, ALS (acetolactate synthase) activities and to determine the several physiological characteristics in the treated plants. Chlorophyll contents in Oryza sativa, Ludwigia prostrata, and Bidens tripartita were increased as the concentration of bensulfuron-methyl increased to 10 μM in media. A notable increase in the reducing sugar content was observed in Aneilema keisak and L. prostrata. Effect of bensufuron-methyl treatment on soluble protein content was different in tested plants. The free amino acid contents in L. prostrata and B. tripartita which are susceptible to this herbicide was significantly increased as the bensulfuron-methyl concentration was increased, however, there was no clear relationship between the free amino acid content and the degree of resistance to sulfonylurea herbicide. The acetolactate synthase activity measured in vitro was decreased drastically as bensulfuron- methyl concentration increased. The I50 values by bensulfuron -methyl for ALS from four tested plant species ranged from 28 nM to 39 nM. Our results of response spectrum and physiological response to sulfonylurea herbicide in Korean weeds may give some clues to set up the appropriate practical schedule for weed control in direct seeding field.
참고문헌 (16)
- Arnon, D. I., "Copper enzyme in isolated chloroplasts polyphenol oxidase in Beta vulgaris", Plant Physiol, 24, pp.1~15 (1949)
- Bernfeld, P, "Amylase, α and β", Method Enzymol, 1, pp.149~158 (1955)
- Bradford, M. M., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Chaleff, R. S., "Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants", Science, 224, pp.1443~1445 (1984)
- Cotterman, J. C., "Rapid metabolic inactivation is the basis for cross- resistance to chlorsulfuron in diclofop-methyl- resistance rigid ryegrass (Lolium rigidum) SR4", 84. Pestic, 1992, pp.43, 182~192
- Davies, D. D., "Amino acid recycling in relation to protein turnover", Plant Physiol, 61, pp.54~58 (1978)
- Hall, L. M., "Cross- resistance of a chlorsulfuron-resistant biotype of Stellaria media to a triazolopyrimidine herbicide", Plant Physiol, 93, pp.962~966 (1990)
- Kwon, C. S., "Absorption, translocation and metabolism of bensulfuron in rice and Cyperus serotinus Rottb", Kor. J. Weed. Sci, 13, pp.75~80 (1993)
- Moore, S, "Amino acid analysis: Aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction", J. Biol. Chem, 243, pp.6281~6283 (1968)
- Pyon, J. Y., "Mechanism of the selectivity and tolerance of sulfonylurea herbicides in rice and weeds", Kor. J. Weed Sci, 11, pp.25~31 (1991)
- Ray T. B., "Site of action of chlorsulfuron", Plant Physiol, 75, pp.827~831 (1984)
- Saari, L. L., "Mechanism of sulfonylurea herbicide resistance in the bradleaf weed, Kochia scoparia", Plant Physiol, 93, pp.55~61 (1990)
- Saari, L., ". Cotterman, W. F. Smith, and M. M. Primiani. 1992. Sulfonylurea herbicide resistance in common chickweed, perennial ryegrass, and Russian thistle", Pestic. Biochem. Physiol, 42, pp.110~118 (1992)
- Shaner, D. L., "Imidazolinones: Potent inhibitors of acetohydroxyacid synthase", Plant Physiol, 76, pp.545~546 (1984)
- Takeda, S., "Mode of selectivity of Londax herbicide(DPX-F5384) in paddy rice", Proc. 10th APWSS Conf., 통권 : 1, pp.156~161 (1985)
- Westerfiels, W, "A colorimetric determination of blood acetoin", J. Biol. Chem, 161, pp.495~502 (1945)