Lily(Lilium longflorum L.) pollen-based transient expression for heterologous protein production
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초록
Transient expression system using lily pollen has been developed for the production of foreign protein. From fully dehisced lily anthers, pollen grains were manually collected, which were vigorously vortexed in the presence of 40-100 μm aluminum oxide particles. The pollen grains with surface wounds were subjected to infiltration with Agrobacterium tumefaciens LBA4404 cells harboring an expression vector containing the erythropoietin(EPO) gene construct, pBI-EPO. The analysis of Southern and cDNA blot hybridization demonstrated that EPO DNA gene was successfully integrated and mRNA synthesis were clearly detected from the transformed pollens. The results suggested that Agrobacterium-mediated pollen transformation system could be used as a convenient biofactory with rapidity.
Abstract
Transient expression system using lily pollen has been developed for the production of foreign protein. From fully dehisced lily anthers, pollen grains were manually collected, which were vigorously vortexed in the presence of 40-100 μm aluminum oxide particles. The pollen grains with surface wounds were subjected to infiltration with Agrobacterium tumefaciens LBA4404 cells harboring an expression vector containing the erythropoietin(EPO) gene construct, pBI-EPO. The analysis of Southern and cDNA blot hybridization demonstrated that EPO DNA gene was successfully integrated and mRNA synthesis were clearly detected from the transformed pollens. The results suggested that Agrobacterium-mediated pollen transformation system could be used as a convenient biofactory with rapidity.
참고문헌 (14)
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