전처리된 억새로부터 효소당화 최적화를 통한 바이오에탄올 생산
Bioethanol Production by Optimal Enzymatic Hydrolysis of Pretreated Miscanthus sinensis var. purpurascens
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초록
한국산 억새를 산, 알칼리 및 증기폭쇄법으로 전처리한 시료의 최적 효소당화조건을 탐색하고, 당화물을 이용한 에탄올 생산성을 구명하였다. 억새의 화학적 조성은 저분자화합물이 5.0%, lignin 17.0%,hemicellulose 55.1%, cellulose 22.7%이었다. 효소 당화율은 단용효소 처리구보다 혼용효소 처리구에서 높았다. Celluclast 처리구는 Viscozyme보다 높은 당화율을 보였으며, 가장 높은 당화율을 보인 효소 처리구는 EM-3(Celluclast 24.3FPU/g 기질+Viscozyme 80.6FBG/g 기질)로 약 70%의 당화율을보였다. 산,알칼리 전처리를 통해 정제된 α-cellulose의 효소 당화율은 1% NaOH 처리 증기폭쇄재 보다 16% 높게 나타났다. 효소 cocktail의 당화에 가장 적합한 온도는 50℃였고, 모든 기질에서 효소 반응시간이 증가할수록 당화율이 증가하였다. 억새의 당화물은 오탄당으로 xylose가 육탄당으로 glucose가 주를 이루었다. S. cerevisiae KCCM 11215를 이용한 당화물의 에탄올 발효에서도 각 효소조합과 시료 전처리가 매우 중요한 것으로 나타났다. 억새시료 1kg 당 350g 이상의 에탄올 생산성을 보였으며,1% NaOH 및 1% KOH를 처리한 증기폭쇄 시료보다 α-cellulose를 기질로 사용한 처리구에서 에탄올생산성이 높았다.
Abstract
This study was carried out to determine the optimal condition of enzymatic hydrolysis fromacid-alkali pre-treated and steam-exploded biomass of Miscanthus sinensis var. purpurascens forethanol production. The acid-alkali pre-treated biomass contained 5% extractive, 17.0% lignin,55.1% hemicellulose and 22.7% cellulose. Saccharification using mixed enzymes was higher thanthat of single enzyme treatment. Saccharification rate by Celluclast 1.5L was highest comparedwith Viscozyme treatment. The highest saccharification was obtained in EM-3(Celluclast24.3FPU/g substrate+Viscozyme 80.6FBG/g substrate) treatment which saccharification ratereached 70%. Saccharification of purified α-cellulose was higher than that of 1% NaOH treatedsteam-exploded biomass. The optimized conditions on enzymatic hydrolysis were withtemperature of 50℃, incubation time of 48 hr. Pretreated sample contained xylose as a majorpentose, and glucose as a major hexose. Ethanol fermentation by Saccaromyces cerevisiae KCCM 11215 relies on the enzyme combination and pretreatment method. Ethanol yield in α-cellulose was highest compared to 1% NaOH and 1% KOH exploded biomass. α-cellulosesample produced the highest in ethanol yields as 350g per 1 kg biomass.
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