Improved Cold Tolerance by Transformation with Soybean SCOF-1 Gene in Populus alba
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The introduction of stress up-regulated genes into heterologous plants is found to conferstability trait to plants in otherwise hostile environments. The soybean cold inducible factor-1gene(SCOF-1) encoding a zinc finger transcription factor was introduced into Populus albaL.(white poplar) by co-culture with Agrobacterium tumefaciens GV3101 that carried the binaryvector, pGA643/SCOF1. The putative P. alba transformed plants were selected on Murashigeand Skoog medium supplemented with 2.22μM 6-benzylaminopurine, 1.18mM carbenicillin, and28.47μM hygromycin. The integration of SCOF-1 into the P. alba genome was also establishedby polymerase chain reaction(PCR), and the expression of the gene was confirmed by reversetranscription PCR. After exposure for 4h at 4℃ followed by 48h recovery at 27℃, thetransgenic plants completely recovered to normal growth status. The chlorophyll content intransgenic plants was higher than that in wild type plants after chilling treatment. However, themoisture content and total phenolics was also not significantly different in transgenic and wildtype plants after cold treatment.
Abstract
The introduction of stress up-regulated genes into heterologous plants is found to conferstability trait to plants in otherwise hostile environments. The soybean cold inducible factor-1gene(SCOF-1) encoding a zinc finger transcription factor was introduced into Populus albaL.(white poplar) by co-culture with Agrobacterium tumefaciens GV3101 that carried the binaryvector, pGA643/SCOF1. The putative P. alba transformed plants were selected on Murashigeand Skoog medium supplemented with 2.22μM 6-benzylaminopurine, 1.18mM carbenicillin, and28.47μM hygromycin. The integration of SCOF-1 into the P. alba genome was also establishedby polymerase chain reaction(PCR), and the expression of the gene was confirmed by reversetranscription PCR. After exposure for 4h at 4℃ followed by 48h recovery at 27℃, thetransgenic plants completely recovered to normal growth status. The chlorophyll content intransgenic plants was higher than that in wild type plants after chilling treatment. However, themoisture content and total phenolics was also not significantly different in transgenic and wildtype plants after cold treatment.
참고문헌 (33)
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