살균제 저항 Bacillus cereus 분리주 포자에 대한 병합처리 효과
Effects of Antimicrobials and Heat Combinational Treatments for Antimicrobial Resistance Bacillus cereus Spores
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살균제 저항성 Bacillus cereus 분리주의 포자에 살균제와 열을 가해 그 병합효과를 비교하였다. 사용된 B. cereus 균주는 차아염소계 처리 후 분리한 균주(S-BC-50, S-BC-52), 과산화수소 처리 후 분리한 균주(S-BC-23, S-BC-48), acetic acid 처리 후 분리한 균주(S-BC-54, S-BC-56)와 표준균주 B. cereus KCTC1661을 사용하였다. B. cereus 분리주의 포자를 다음 조건으로 처리하였다; i) 0.56% 과산화수소수(HP), ii) 3% acetic acid(AA), iii) 80oC 열처리(H80), iv) 90o 열처리(H90), v) HP+H80, vi) HP+H90, vii) AA+H80, viii) AA+H90. 포자를 HP와 AA 처리에 노출시킨 결과 포자가 감소하지 않았지만, H80과 H90 처리에서는 1-2 log CFU/mL가 감소하였다(P<0.05). 병합처리 (HP+H80, HP+H90, AA+H80, AA+H90)에 포자를 적용시킨 경우, 포자수가 3-6 log CFU/mL 감소하였지만, AA+H80과 AA+H90은 AA만 처리했을 때보다 오히려 포자 제어가 감소했다. 따라서 HP와 열처리를 병합했을 때는 B. cereus 포자를 불활성화하기에 효과적이지만, AA의 경우 B. cereus 포자가 열처리에 대한 저항성을 증가시키는 것으로 사료된다.
Abstract
Effects of antimicrobials and heat combinational treatment for antimicrobial resistance Bacillus cereus spores were compared. B. cereus strains were isolated after sodium hypochlorite(strains S-BC-50 and S-BC-52), hydrogen peroxide(strains S-BC-23 and S-BC-48), and acetic acid(strains S-BC-54 and S-BC-56) treatments. The spores of B. cereus isolates and B. cereus KCTC1661(standard strain) were treated following conditions; i) 0.56% hydrogen peroxide(HP), ii) 3% acetic acid(AA), iii) heating at 80oC(H80), iv) heating at 90oC(H90), v) HP+H80, vi) HP+H90, vii) AA+H80, and viii) AA+H90. Spore counts were not decreased after HP and AA treatments but H80 and H90 reduced(P<0.05) by 1-2 log CFU/mL. The combinational treatments(HP+H80, HP+H90, AA+H80, and AA+H90) showed decrease to 3-6 log CFU/mL of spore counts, but AA+H80 and AA+H90 treatments showed less decrease than AA treatment. The results indicate that combination of HP with heating can effectively inactivate B. cereus spores but AA may increase heat resistance of B. cereus spores.
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