Effects of Anthelmintic Plant Extracts on Ruminal Fermentation Characteristics, Bacterial Diversity and Methane Production in vitro
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The objective of this study was to know the in vitro effects of supplemental anthelminticplant extracts on the inhibition of protozoa for reducing methane production in the rumen. Afistulated Holstein cow was used as a donor of rumen fluid. The plant extracts (Lonicerajaponica, Zanthoxylum piperitum, Pyrethrum, Torreya nucifera, Ruta graveolens) known to haveanthelmintic effect were added to the in vitro fermentation bottles containing the rumen fluidand medium. The rumen protozoal population was depressed by the addition of Pyrethrum,Torreya nucifera and Ruta graveolens. The methane production was also significantly (p<0.05)reduced by addition of Pyrethrum (2.20 ml/g DM), Torreya nucifera (2.36 ml/g DM) and Rutagraveolens (2.20 ml/g DM). The microbial growth in the treatments of Ruta graveolens orZanthoxylum piperitum was the greatest after 12 h and 24 h incubations, respectively. Theresults of this study indicated that anthelmintic plant extracts appeared to reduce methaneproduction by inhibition of ruminal protozoa related with the methanogens living endosymbioticin protozoal cells.
Abstract
The objective of this study was to know the in vitro effects of supplemental anthelminticplant extracts on the inhibition of protozoa for reducing methane production in the rumen. Afistulated Holstein cow was used as a donor of rumen fluid. The plant extracts (Lonicerajaponica, Zanthoxylum piperitum, Pyrethrum, Torreya nucifera, Ruta graveolens) known to haveanthelmintic effect were added to the in vitro fermentation bottles containing the rumen fluidand medium. The rumen protozoal population was depressed by the addition of Pyrethrum,Torreya nucifera and Ruta graveolens. The methane production was also significantly (p<0.05)reduced by addition of Pyrethrum (2.20 ml/g DM), Torreya nucifera (2.36 ml/g DM) and Rutagraveolens (2.20 ml/g DM). The microbial growth in the treatments of Ruta graveolens orZanthoxylum piperitum was the greatest after 12 h and 24 h incubations, respectively. Theresults of this study indicated that anthelmintic plant extracts appeared to reduce methaneproduction by inhibition of ruminal protozoa related with the methanogens living endosymbioticin protozoal cells.
참고문헌 (32)
- Abe, M., "Modifying method of counting protozoa", Jpn. J. Zootec. Sci, 43, pp.535~536 (1972)
- Ahn, Y. S., "Isolation and identification of active antimicrobial substance against Listeria monocytogenes from Ruta graveolens Line", Korean J. Food Sci. Technol, 32, pp.1379~1388 (2000)
- 조영제, "Rue(Ruta graveolens L.) 추출물의 Helicobacter pylori에 대한 항균활성과 생리활성효과", 한국식품영양과학회지, 34(4), pp.460~465 (2005), 한국식품영양과학회
- Chung, B. S., "Studies on the sterol components of Torreya nut of Korea", Yakhak Hoeji, 22, pp.87~90 (1978)
- Dehority, B. A., "Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria", J. Bacteriol, 89, pp.1515~1520 (1965)
- Duncan, D. B., "Multiple range and multiple F test", Biometrics, 11, pp.1~6 (1995)
- Erwin, E. S., "Volatile fatty acid analysis of blood and rumen fluid by gas chromatography", J. Dairy Sci, 44, pp.1768~1771 (1961)
- Fedorah, P. M., "A simple apparatus for measuring gas production by methanogenic cultures in serum bottles", Environ. Technol. Lett, 4, pp.425~432 (1983)
- Finlay, B. J., "Some rumen ciliates have endosymbiotic methanogens", FEMS Microbiol. lett, 117, pp.157~162 (1994)
- Houghton, P. J., "A clinical evaluation of the Chinese herbal mixture 'Aden-I' for treating respiratory infections", Phytother. Res, 7, pp.384~386 (1993)
- 전호성, "비자(Torreya nucifera) 추출물의 생리활성", 한국식품영양과학회지, 38(1), pp.1~8 (2009), 한국식품영양과학회
- Johnson, K. A., "Methane emissions from cattle", J. Anim. Sci, 73, pp.2483~2492 (1995)
- Jouany, J. P., "Manipulation of microbial activity in the rumen", Arch. Anim. Nutr, 46, pp.133~153 (1994)
- 신택순, "초피첨가 전통장류의 항균 및 항암활성", 생명과학회지, 17(8), pp.1121~1128 (2007), 한국생명과학회
- Kim, S. Y., "Antioxidant activities of selected oriental herb extracts", J. Am. Oil Chem. Soc, 71, pp.633~640 (1994)
- Kim, Y. D., "A study on the ichthyotoxic constituents of chopi (Zanthoxylum piperitum DC)", J. Korean Soc. Food Sci. Nutr, 22, pp.617~620 (1993)
- Lee, S. J., "Korean folk medicine", pp.6~ (1966), Publishing Center of Seoul National University
- 이신자, "Effects of Synbiotics Containing Anaerobic Microbes and Prebiotics on In vitro Fermentation Characteristics and In situ Disappearance Rate of Fermented-TMR", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 24(11), pp.1577~1586 (2011), 아세아·태평양축산학회
- Lee, T. B., "Illustrated flora of Korea", pp.56~ (1993), Hyang Moon Sa
- Lowe, S. E., "Growth of anaerobic rumen fungi on defined and semi-defined media lacking rumen fluid", J. Gen. Microbiol, 131, pp.2225~2229 (1985)
- Machmüller, A., "Effect of coconut oil and defaunation treatment on methanogenesis in sheep", Reprod. Nutr. Dev, 43, pp.41~55 (2003)
- McCrabb, G. J., "Inhibiting methane production in Brahman cattle by dietary supplementation with a novel compound and the effects on growth", Aust. J. Agric. Res, 48, pp.323~329 (1997)
- McDougall, E. I., "Studies on ruminant saliva. 1. The composition and output of sheep’s saliva", Biochem. J, 43, pp.99~109 (1948)
- Makoto Mitsumori, "Control of Rumen Microbial Fermentation for Mitigating Methane Emissions from the Rumen", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 21(1), pp.144~154 (2008), 아세아·태평양축산학회
- Nagaraja, T. G., "Biotechnology in Animal Feeds and Animals Feeding", pp.173~204 (1995), VCH Verlagsgesellschaft
- Nellot, L., "Effect of 2-bromoethanesulfonic acid and Peptostreptococcus productus ATCC 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective by inhibition of methanogens", Appl. Environ. Microbiol, 63, pp.194~200 (1997)
- SAS Inc, "SAS user’s guide", SAS Inst. Inc, ~ (2002)
- 신정희, "금은화 추출물을 이용한 미생물 발효 생성물의 항산화 특성", 동아시아식생활학회지, 22(1), pp.95~102 (2012), 동아시아식생활학회
- Stumm, C. K., "Association of methanogenic bacteria with ovine rumen ciliates", Br. J. Nutr, 47, pp.95~100 (1982)
- Takenaka, A, "Changes in the population of some functional groups of rumen bacteria including methanogenic bacteria by changing the rumen ciliates in calves", J. Appl. Microbiol, 41, pp.377~387 (1995)
- Teferedegne, B., "New perspectives on the use of tropical plants to improve ruminant nutrition", Proc. Nutr. Soc, 59, pp.214~209 (2000)
- Van Nevel, C. J., "Biotechnology and animal feeds and feeding", pp.329~349 (1995), VCH Publishers Inc