Development and Evaluation of a Rapid Dipstick Assay for Serodiagnosis of Bovine Brucellosis
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Fast, cheap and sufficient serodiagnostic tools needs to be developed for the early detectionof brucellosis. Currently the tools cannot differentiate an active infection from vaccinated, norcan it differentiate other bacterial infections with lipopolysaccharides, especially Yersiniainfections. In this study, we purified recombinant outer membrane protein 10 and 28(rOmp10,rOmp28), and a dipstick assay(indirect or sandwich) was constructed with single(rOmp10 orrOmp28) and combined rOmps(rOmp10 and rOmp28) from Brucella(B.) abortus 544 to evaluatebovine Brucella positive serum collected during the beginning of the Korean outbreak from2006 to 2015. In application with single rOmp, rOmp10(70%; indirect, 92.11%; sandwichdipstick) and rOmp28(72.5%; indirect, 86.84%; sandwich dipstick) had comparable results. Inaddition, results indicated that dipstick with combined rOmps(rOmps10 and rOmp28) weresuperior in detecting positive serum samples, at 85% indirect and 100% sandwich dipstick. Surprisingly, the results were the same in detecting negative results at 97.78% for both singleand combined indirect dipsticks. The dipstick tools with rOmp10 and rOmp28 would be usefulfor a rapid screen method for bovine brucellosis.
Abstract
Fast, cheap and sufficient serodiagnostic tools needs to be developed for the early detectionof brucellosis. Currently the tools cannot differentiate an active infection from vaccinated, norcan it differentiate other bacterial infections with lipopolysaccharides, especially Yersiniainfections. In this study, we purified recombinant outer membrane protein 10 and 28(rOmp10,rOmp28), and a dipstick assay(indirect or sandwich) was constructed with single(rOmp10 orrOmp28) and combined rOmps(rOmp10 and rOmp28) from Brucella(B.) abortus 544 to evaluatebovine Brucella positive serum collected during the beginning of the Korean outbreak from2006 to 2015. In application with single rOmp, rOmp10(70%; indirect, 92.11%; sandwichdipstick) and rOmp28(72.5%; indirect, 86.84%; sandwich dipstick) had comparable results. Inaddition, results indicated that dipstick with combined rOmps(rOmps10 and rOmp28) weresuperior in detecting positive serum samples, at 85% indirect and 100% sandwich dipstick. Surprisingly, the results were the same in detecting negative results at 97.78% for both singleand combined indirect dipsticks. The dipstick tools with rOmp10 and rOmp28 would be usefulfor a rapid screen method for bovine brucellosis.
참고문헌 (17)
- Acha PN, "Zoonosis and communicable disease common to man and animals", pp.28~45 (2003), Pan American Health Org
- Clavijo E, "Comparison of a dipstick assay for detection of Brucella-specific immunoglobulin M antibodies with other tests for serodiagnosis of human brucellosis", Clin. Diagn. Lab. Immunol, 10(4), pp.612~615 (2003)
- Corbel MJ, "Recent advances in brucellosis", J. Med. Microbiol, 46(2), pp.101~103 (1997)
- Delpino MV, "Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB", c mice, 2007, pp.25, 6721~6729
- Lee BY, "Surveillance and control of bovine brucellosis in the Republic of Korea during 2000-2006", Prev. Vet. Med, 90, pp.66~79 (2006)
- Lim JJ, "Evaluation of recombinant 28 kDA outer membrane protein of Brucella abortus for the clinical diagnosis of bovine brucellosis in Korea", J. Vet. Med. Sci, 74, pp.687~691 (2012)
- Lindler LE, "Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis", Infect. Immun, 64(7), pp.2490~2499 (1996)
- Lubroth J, "Veterinary vaccines and their use in developing countries", Rev. Sci. Tech, 26(1), pp.179~201 (2007)
- Mert A, "The sensitivity and specificity of Brucella aggulination tests", Diagn. Microbiol. Infect. Dis, 46(4), pp.241~243 (2003)
- Nielsen K, "Diagnosis of brucellosis by serology", Vet. Microbiol, 90(1-4), pp.447~459 (2002)
- "OIE Terrestrial Manual 2012" (2002)[URL]
- Paek SH, "Development of rapid one-step immunochromatographic assay", Methods, 22(1), pp.53~60 (2000)
- Pappas G, "The new global map of human brucellosis", Lancet. Infect. Dis, 6(2), pp.91~99 (2006)
- Simborio HLT, "Evaluation of the combined use of the recombinant Brucella abortus Omp10, Omp19 and Omp28 proteins for the clinical diagnosis of bovine brucellosis", Microb. Pathog, 16, pp.414~416 (2015)
- Smits HL, "Development and evaluation of a rapid dipstick assay for serodiagnosis of acute human brucellosis", J. Clin. Microbiol, 37, pp.4178~4182 (1999)
- Wee SH, "Emergence of brucellosis in cattle in the Republic of Korea", Vet. Rec, 162, pp.556~557 (2008)
- Weynants V, "Infection of cattle with Yersina enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic test", Vet. Microbiol, 48(2), pp.101~112 (1996)