단일염기다형성 정보를 이용한 재래흑염소 경상대계통의 유효집단 크기 추정
Effective Population Size using Single Nucleotide Polymorphism Information in Korean Native Black Goat
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초록
본 연구에 이용되는 국내 재래 흑염소는 유전적 자원을 보존하고 품종을 유지하기 위하여 소규모 집단에서 고도의 근친교배를수행하고 있어 유전적 자질의 다양성이 저해되고 있을 것으로 우려되었다. 따라서 본 연구는 국내 재래흑염소 경상대계통 집단의유전체 정보를 활용하여 재래흑염소 집단의 유전적 특성과 유효집단의 크기를 추정하고자 실시하였다. 국내 재래 흑염소의 유전체정보는 Illumina Goat SNP 50k chip (illumina, inc., San Diego, CA)의 정보를 분석하여 연구에 이용하였다. 각 염색체의 인접 표지인자와의 연관불평형 (Linkage Disequilibrium)은 0.225로 추정이 되었다. 또한, 표지인자 사이의 거리가 증가함에 따라 연관불평형의값은 감소되는 것으로 나타났다. 국내 재래 흑염소의 유효집단크기는 최근의 세대로 오는 경우 감소하는 추세를 보였으며, 13세대에서유효집단의 크기는 29두로 추정되었다. 재래 흑염소 경상대계통은 낮은 연관불평형 값과 유효집단 크기를 보여 유전적 자원의 다양성이낮을 것으로 보이며, 이를 해결하기 위한 계획적인 교배와 품종 집단의 크기를 키우기 위한 대책이 필요할 것으로 사료된다.
Abstract
This study was some possibility that the variety of genetic quality of the Korean native black goat population might have been damaged because the small group of goat used in this study had been under highly intensive inbreeding with the aim of preserving its genetic resources and keeping the breed. Accordingly, this study was performed to estimate genetic characteristics and size of effective population of Korean native black goat using the genetic information of the group of goat of GNU strain population. The genetic information on Korean native black goat used by this study was Illumina Goat SNP 50k chip (Illumina, Inc., San Diego, CA). The linkage disequilibrium with adjoining markers of each chromosome was estimated to be 0.225. And, it was found that, as the distance between markers gets farther, linkage disequilibrium value gets smaller. It was also found that the effective population size of Korean native black goat becomes smaller with the increase of the number of its generations, and that the effective population size for the 13th generation is 29 heads. Considering the low linkage disequilibrium value and the small size of effective population of the group of goat of GNU strain population, we can assume that the group may have low variety of genetic resources. To solve such a problem, it seems necessary to adopt measures to do planned crossbreeding and increase the size of the group.
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