전형매 가계 내 유전체정보량에 따른 유전체육종가 추정 및 정확도 분석
Estimated of Genomic Estimated Breeding Value and Accuracy Analysis according to the Amount of Genotypes in the Full-Sib Family
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참고문헌
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초록
한우 개량에 있어서 추정된 유전체육종가와 정확도는 선발에 중요한 지표로 사용되며, 최근 육종가 추정에 있어 정확도의 신뢰도를 높이기위해 혈통과 유전체정보를 이용한 연구가 활발히 진행되고 있다. 따라서, 본 연구는 가계 내 유전체정보량에 따라 유전체정보가 미포함된 개체의정확도 변화를 확인하고자 동일한 부모를 가진 한우 10두로 구성된 전형매 가계 3개를 수집하였으며, 각 가계 별로 유전체정보량을 10두, 8두, 6두, 4두, 2두씩 무작위로 선별하여 5가지의 검정집단으로 가정한 후 참조집단 14,225두를 이용하여 single step genomic best linear unbiased prediction (ssGBLUP)을 통해 genomic estimated breeding value (GEBV) 및 정확도를 추정하였다. 각 검정집단과 참조집단의 혈통 및 유전체정보를 이용하여 H-matrix를 구축하였고, BLUPF90 program을 사용하여 도체중, 등심단면적, 등지방두께, 근내지방도의 GEBV 및 정확도를 추정하였다. 첫 번째 가계를 대상으로 살펴보면, 검정집단에서 유전체정보를 보유하고 있는 10두의 GEBV 평균 정확도는 도체중 0.734, 등심단면적 0.717, 등지방두께 0.712, 근내지방도 0.745로 추정되었다. 이후 2두씩 무작위로 유전체정보를 제거하여 추정한 GEBV 정확도를 살펴보면, 유전체정보를보유한 개체의 경우 정확도의 변화가 나타나지 않았지만, 유전체정보가 미포함된 개체의 정확도가 평균 0.114 ~ 0.168 낮게 추정되었다. 가계내 유전체정보량에 따른 유전체정보가 미포함된 개체의 GEBV 평균 정확도는 도체중 0.604 ~ 0.576, 등심단면적 0.6 ~ 0.573, 등지방두께 0.599 ~ 0.572, 근내지방도 0.607 ~ 0.578로 평균 0.009씩 감소하는 것을 확인하였다. 이를 통해 가계 내 유전체정보량이 개체 별 유전체정보 유무와는상관없이 GEBV의 정확도 추정에 큰 영향이 없었으며, 신뢰도가 높은 GEBV 추정을 위해서는 개체 별 유전체정보의 유무가 더 큰 영향을 미친다는것을 확인하였다.
Abstract
The genomic estimated breeding value (GEBV) and accuracy of Korean beef (Hanwoo) are used as important indicators for selection, and recent studies using pedigree and genotype are being conducted to increase the reliability of the accuracy. Therefore, in this study, three families consisting of 10 Hanwoo with the same parents were collected to check the change in accuracy of individuals without genotypes according to the amount of genotypes in the family. GEBV and accuracy were estimated through single-step genomic best linear unbiased prediction (ssGBLUP) using 14,225 reference groups after assuming 5 test groups by selecting 10, 8, 6, 4, and 2 genotypes for each family. An H-matrix was constructed using pedigree and genotype of the groups, and the GEBV accuracy of carcass weight (CWT), eye muscle area (EMA), backfat thichness (BFT) and marbling score (MS) were estimated using the BLUPF90 program. Looking at the first family, the average GEBV accuracy of 10 heads with a genotype in the test group was estimated to be 0.734, 0.717, 0.712 and 0.745 in CWT, EMA, BFT and MS, respectively. Then, looking at the GEBV accuracy estimated by removing the genotype two by two, there was no change in accuracy in the case of individuals with a genotype, but the accuracy of the individuals without a genotype was estimated to be 0.114~0.168 lower on average. It was confirmed that the average GEBV accuracy of individuals without a genotype was 0.604~0.576, 0.6~0.573, 0.599~0.572 and 0.607~0.578 in CWT, EMA, BFT and MS, respectively. And it was confirmed that they decreased by 0.009 on average. Through this, although the amount of genotypes within a group did not have a significant effect on accuracy regardless of the presence of individual genotypes, the presence of individual genotypes had a greater effect on GEBV with high reliability.
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