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메티실린 내성 황색포도알균 검출을 위한 ChromIDTMMRSA 평가

Evaluation of ChromID MRSA for the Detection of Methicillin-resistant Staphylococcus aureus

강대혁|김민정|최태열|박재임
대한임상미생물학회 (Korean Society of Clinical Microbiology)|2009.12|Annals of Clinical Microbiology|제12권 제4호|pp. 169-173

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참고문헌

키워드

PerformanceDetectionMethicillin-resistant Staphylococcus aureus (MRSA)Culture medium

초록

배경: 메티실린 내성 황색포도알균(methicillin-resistant Staphylococcus aureus, MRSA)은 병원 및 지역사회감염에서 중요한 병원균이므로, MRSA 보균자의 신속 발견은 병원에서 중요한 병원감염관리 정책 중 하나이다. 연구자들은 MRSA 조기 검출을 위하여 ChromID MRSA (bioMerieux, Marcy I’Etoile, France)의 민감도와 특이도를 평가하였다. 방법: 190개의 검체를 신생아 중환자실에 입원한 미숙아의 전비공에서 채취하였다. 검체는 oxacillin 6 mg/L가 함유된 Broth enrichment (BE)에 접종 후 oxacillin 6 mg/L 함유한 mannitol salt agar (MSAO)과 ChromID MRSA에 동량(100μL)씩 접종하고, 나머지를 하룻밤 배양하여 ChromID MRSA에 동량 접종하였다. 결과: BE에서 전체 190개 중 21개(11%)의 검체에서 MRSA가 검출되었다. 배양 24시간 후 MSAO의 민감도/특이도는 52%/98%, ChromID MRSA는 62%/100%였으며, 배양 48시간 후에 MSAO의 민감도/특이도는 62%/92%, ChromID MRSA는 81%/99%였다. 결론: ChromID MRSA는 MRSA를 신속 분리 동정하기에 유용한 선택 배지이다.

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant pathogen in both nosocomial and community settings, and screening for a carrier is an important infection control practice in many hospitals. We evaluated the sensitivity and specificity of the ChromID MRSA assay (bioMérieux, Marcy I’Etoile, France). Methods: A total of 190 clinical samples were collected from the anterior nares of premature infants in a newborn intensive care unit (N-ICU). Equal volumes (100μL) of the samples were inoculated on mannitol salt agar with oxacillin 6 mg/L (MSAO) and ChromID MRSA after emulsifying the screening swab in brain-heart Infusion broth with oxacillin 6 mg/L (BE). The specimens in BE were subcultured on ChromID MRSA after an overnight incubation. Results: Twenty-one of 190 samples (11%) was positive for MRSA by BE. After a 24 h incubation, the sensitivity/specificity of MSAO was 52%/98% and that of ChromID MRSA was 62%/100%, and at 48 h, the sensitivity/specificity of MSAO was 62%/92% and that of ChromID MRSA was 81%/99%. Conclusion: ChromID MRSA is a useful selective medium for the rapid isolation and identification of MRSA.

참고문헌 (29)

  1. Emori TG, "An overview of nosocomial infections, including the role of the microbiology laboratory", Clin Microbiol Rev, 6, pp.428~442 (1993)
  2. Smith PW, "HEA", APIC guideline: Infection prevention and control in the long-term care facility, July 2008, 2008, pp.29, 785~814
  3. Muto CA, "SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and Enterococcus", Infect Control Hosp Epidemiol, 24, pp.362~386 (2003)
  4. Coia JE, "Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities", Hosp Infect, 63(1), pp.S1~S44 (2006)
  5. Malhotra-Kumar S, "Current trends in rapid diagnostic for methicillin-resistant Staphylococcus aureus and glycopeptides-resistant enterococcus species", J Clin Microbiol, 46, pp.1577~1587 (2008)
  6. 이혁민, "2004년도 국내 12개 병원에서 분리된 주요 세균의 항균제 내성율", 대한임상미생물학회지, 8(1), pp.66~73 (2005), 대한임상미생물학회
  7. Lee SO, "Korean nosocomial infections surveillance system (KONIS) report: data summary from July through September 2006", Korean J Nosocomial Infect Control, 11, pp.113~128 (2006)
  8. Compernolle V, "Combined use of Pastorex Staph-Plus and either of two new chromgenic agar, MRSA ID and CHROMagar MRSA, for detection of methicillin- resistant Staphylococcus aureus", J Clin Microbiol, 45, pp.154~158 (2007)
  9. Perry JD, "Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus", J Clin Microbiol, 42, pp.4519~4523 (2004)
  10. Nahimana I, "Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus", Clin Microbiol Infect, 12, pp.1168~1174 (2006)
  11. Diederen BM, "Performance of MRSA ID, a new chromogenic medium for detection of methicillin-resistant Staphylococcus aureus", J Clin Microbiol, 44, pp.586~588 (2006)
  12. Blanc DS, "Evaluation of a novel medium for screening specimens from hospitalized patients to detect methicillin- resistant Staphylococcus aureus", J Clin Microbiol, 41, pp.3499~3502 (2003)
  13. van Loo IHM, "Evaluation of a chromogenic agar (MRSASelect) for the detection of methicillin-resistant Staphylococcus aureus with clinical samples on the Netherlands", Med Microbiol, 56, pp.491~494 (2007)
  14. Louie L, "Evaluation of three rapid methods for detection of methicillin resistance in Staphylococcus aureus", Clin Microbiol, 38, pp.2170~2173 (2000)
  15. Performance standards for antimicrobial susceptibility testing; Nineteenth informational supplement, "Clinical and Laboratory Standards Institute", CLSI, ~, pp.CLSI M100-S19 (2009)
  16. Murakami K, "Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction", J Clin Microbiol, 29, pp.2240~2244 (1991)
  17. van Hal SJ, "Methicillin-resistant Staphylococcus aureus (MRSA) detection: comparison of two molecular methods (IDI-MRSA PCR assay and Genotype MRSA direct PCR assay) with three selective MRSA agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for use with infection-control swabs", J Clin Microbiol, 45, pp.2486~2490 (2007)
  18. Warren DK, "Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay", J Clin Microbiol, 42, pp.5578~5581 (2004)
  19. Boyce JM, "Comparison of BD GeneOhm methicillin- resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains", J Clin Microbiol, 46, pp.350~351 (2008)
  20. Wolk DM, "Multicenter evaluation of the Cepheid Xpert methicillin- resistant Staphylococcus aureus (MRSA) test as a rapid screening method for detection of MRSA in nares", J Clin Microbiol, 47, pp.758~764 (2009)
  21. Flayhart D, "Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance culture of the anterior nares", J Clin Microbiol, 43, pp.5536~5540 (2005)
  22. Velasco D, "Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus", J Antimicrob Chemother, 55, pp.379~382 (2005)
  23. Kluytmans J, "Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks", Clin Microbiol Rev, 10, pp.505~520 (1997)
  24. Noble WC, "Some aspects of nasal carriage of staphylococci", J Clin Pathol, 17, pp.79~83 (1964)
  25. Stoakes L, "Prospective comparison of a new chromogenic medium, MRSASelect, to CHROMagar MRSA and mannitol-salt medium supplemented with oxacillin or cefoxitin for detection of methicillin- resistant Staphylococcus aureus", J Clin Microbiol, 44, pp.637~639 (2006)
  26. Lally RT, "Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus", J Clin Microbiol, 22, pp.501~504 (1985)
  27. Lagacé-Wiens PR, "Reductions in workload and reporting time by use of methicillin-resistant Staphylococcus aureus screening with MRSASelect medium compared to mannitol-salt medium supplemented with oxacillin", J Clin Microbiol, 46, pp.1174~1177 (2008)
  28. Carson J, "Interpretation of MRSASelect screening agar at 24 hours of incubation", J Clin Microbiol, 47, pp.566~568 (2009)
  29. Krishna BV, "Evaluation of chromogenic MRSA medium, MRSASelect and oxacillin resistance screening agar for the detection of methicillin-resistant Staphylococcus aureus", Clin Pathol, 61, pp.841~843 (2008)

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