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우리나라에서 분리되는 결핵균의 분자역학적 특성

Characteristics of Molecular Strain Typing of Mycobacterium tuberculosis Isolated from Korea

최고은|김영대|장철훈|장미희
대한임상미생물학회 (Korean Society of Clinical Microbiology)|2011.06|Annals of Clinical Microbiology|제14권 제2호|pp. 41-47

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키워드

Mycobacterium tuberculosisMolecular strain typingBeijing family

초록

결핵의 집단 발병이나 검사실의 교차 오염의 검출, 결핵의 재감염과 재활성화의 구분 등에서 결핵균을 분자생물학적으로 구분하는 것이 중요하다. 여기서는 현재 이용되는 결핵균의 균주 구분 방법을 알아보고, 한국에서 검출되는 균주의 분포를 알아본다. IS6110-restriction fragment length polymorphism (RFLP) 방법은 표준 방법으로 간주된다. 전세계적으로 풍부한 자료가 있고 균주 구별 능력은 뛰어나나 시간과 노력이 많이 든다. Spoligotyping은 direct repeat (DR) 사이사이에 있는 염기 서열의 존재 여부로 구분한다. PCR을 기반으로 하기 때문에 의심 초기에 검사할 수 있고 결과를 숫자로 표시할 수 있어서 좋다. Beijing family의 균주들은 모두 같은 형으로 나타나는 것이 단점이다. Mycobacterial interspersed repetitive unit-variable number of tandem repeat (MIRU-VNTR) 방법도 PCR을 기반으로 하고 숫자로 결과를 표현할 수 있어 좋다. 한국에서 분리되는 균주의 80∼87%가 Beijing family에 속하는 균주이다.

Abstract

Molecular strain typing of Mycobacterium tuberculosis is important for the detection of outbreaks of tuberculosis and laboratory cross contamination, as well as the differentiation between re-infection and reactivation of tuberculosis. In the present review, the authors investigated the currently available typing methods for M. tuberculosis and the current status of strain distribution in Korea. IS6110-restriction fragment length polymorphism (RFLP), which is considered a standard method, is based on numbers and positions of the insertion sequence, IS6110. The method has an excellent discriminatory power with a considerable amount of worldwide data, although it is time-consuming and labor-intensive. Spoligotyping is based on the presence or absence of spacer sequences between direct repeat (DR) regions. PCR amplification allows for the possibility of application in the early suspicious stage. The data can be easily digitized; however, it shows identical profiles in Beijing family strains. Mycobacterial interspersed repetitive unit-variable number of tandem repeat (MIRUVNTR) is another PCR-based genotyping method with a good discrimination power whose data can also be easily digitized. In Korea, the prevalence of Beijing family strains have been as high as 80 to 87%.

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관련 논문AI추천

Strain Typing of Mycobacterium tuberculosis Isolates from Korea by Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats송은주, 윤경원, 이은엽, 장철훈, 최고은, 황인경대한진단검사의학회 · Annals of Laboratory Medicine2009 · 제29권 제4호Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea김성률, 김정만, 신보문, 이선호, 장미희, 장철훈, 최고은대한진단검사의학회 · Annals of Laboratory Medicine2011 · 제31권 제4호Application of Single-nucleotide Polymorphism and Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats Analyses to Clinical Mycobacterium tuberculosis Isolates from Korea김문범, 김영대, 이선민, 이은엽, 이종윤, 장미희, 장철훈, 조현정, 최고은대한진단검사의학회 · Annals of Laboratory Medicine2011 · 제31권 제1호임상 검체에서 분리한 Mycobacterium tuberculosis Complex 중에서 Mycobacterium tuberculosis의 빈도어영, 김종배, 조현미대한임상미생물학회 · Annals of Clinical Microbiology2020 · 제23권 제1호호흡기 검체에서 분리된 결핵균의 항결핵제 내성 실태: 국내 다기관 연구김자영, 박연준, 신종희, 이남용, 이미애, 장철훈대한임상미생물학회 · Annals of Clinical Microbiology2013 · 제16권 제1호결핵 진단에 있어서 In-house 중합효소연쇄반응법과 전통적인 진단 방법간의 비교검토박수연, 서진태, 양희영, 이광길, 이희주대한진단검사의학회 · Annals of Laboratory Medicine2006 · 제26권 제3호

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