금속표면이 항생제 내성균주의 생육억제에 미치는 영향
Inhibitory Effect of Metal Surface on the Antimicrobial Resistance Microorganism
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
배경: 본 연구는 국내 병원에서 분리된 methicillin 내성 Staphylococcus aureus (MRSA), vancomycin 내성 Enterococcus faecium (VREFM) 및 다제 내성 multidrug-resistant Pseudomonas aeruginosa (MRPA) 등에 대한 구리, 유기(구리 78%, 주석 22%), stainless steel의 살균력을 비교 실험하여, 국내 병원 환경에 의한 항생제 내성 균주 교차 감염을 예방하는 데 구리와 유기의 활용성을 분석하고자 하였다. 방법: MRSA, VREFM, MRPA 균주는 2017년 의정부성모병원에서 분리·동정된 wild type 3균주씩을 혼합하여 사용하였으며 모두 항생제 다제 내성 균주로 나타났다. MRSA, VREFM, MRPA 균주 각각을 Tryptic soy broth (Oxoid, England)에 접종하여 35°C에서 24시간 배양한 후, 각각 초기 접종균액의 균수를 105 log CFU/mL로 조정하였다. 구리, 유기, stainless steel 용기에 준비된 MRSA, VREFM, MRPA 접종균액 100 mL씩을 각각 접종한 후, 습도가 유지되도록 덮개를 덮은 후 35°C incubator에서 초기부터 9일까지 생존균수를 측정하였다. 결과: 본 연구결과 국내에서 분리된 항생제 다제 내성 MRSA, VREFM, MRPA 균주에 대한 구리 및 유기의 살균효과를 확인하였다. Stainless steel의 살균력은 구리 및 유기에 비해 매우 미약하였고, MRSA 및 VREFM에 비해 MRPA의 살균효과가 크게 나타났다. 결론: 교차감염을 예방하기 위하여 병원 내 문손잡이, 수도꼭지, 침대레일 등에 구리나 유기를 적용하여, 장기간 실험을 진행하고 있는 국외 사례와 같이, 국내 병원에서도 구리와 유기를 이용한 병원 내 교차 감염 예방 연구가 필요하다고 생각한다. [Ann Clin Microbiol 2018;21:80-85]
Abstract
Background: The aim of this study was to comparatively evaluate the bactericidal effects of copper, brass (copper 78%, tin 22%), and stainless steel against methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VREFM), and multidrug-resistant Pseudomonas aeruginosa (MRPA). Methods: The isolates (MRSA, VREFM, MRPA) used in this study were mixed wild type 3 strains isolated from patients treated at Uijeongbu St. Mary’s Hospital in 2017. These strains showed patterns of multidrug resistance. The lyophilized strains were inoculated into and incubated for 24 hr in tryptic soy broth at 35°C. The initial bacterial inoculum concentration was adjusted to 105 CFU/mL. A 100-mL bacterial suspension was incubated in containers made of brass (copper 78%, tin 22%), copper (above 99% purity), and stainless steel at 35°C. Viable counts of bacteria strains were measured for 9 days. Results: In this study, the bactericidal effects of copper and brass on MRSA, VREFM, and MRPA were verified. The bactericidal effect of stainless steel was much weaker than those of copper and brass. The bactericidal effect was stronger on MRPA than on MRSA or VREFM. Conclusion: To prevent cross infection of multidrug resistant bacteria in hospitals, further studies of longer duration are needed for testing of copper materials on objects such as door knobs, faucets, and bed rails. (Ann Clin Microbiol 2018;21:-85)
참고문헌 (23)
- 김정목, "한국인 환자에서 분리한 Helicobacter pylori 균주의 항생제 내성률", 대한소화기학회지, 47(5), pp.337~349 (2006), 대한소화기학회
- Jevons MP, "“Celbenin” - resistant staphylococci", Br Med J, 1, pp.124~125 (1961)
- Grass G, "Metallic copper as an antimicrobial surface", Appl Environ Microbiol, 77, pp.1541~1547 (2011)
- Burton DC, "Methicillin-resistant Staphylococcus aureus central line-associated bloodstream infections in US intensive care units, 1997-2007", JAMA, 301, pp.727~736 (2009)
- 이혁민, "2004년도 국내 12개 병원에서 분리된 주요 세균의 항균제 내성율", Annals of Clinical Microbiology, 8(1), pp.66~73 (2005), 대한임상미생물학회
- Moon HW, "Antimicrobial susceptibility profiles of Staphylococcus aureus isolates classified according to their origin in a tertiary hospital in Korea", Am J Infect Control, 42, pp.1340~1342 (2014)
- Stosor V, "The management and prevention of vancomycin-resistant enterococci", Infect Med, 13, pp.487~493 (1996)
- Breathnach AS, "Multidrug-resistant Pseudomonas aeruginosa outbreaks in two hospitals: association with contaminated hospital waste-water systems", J Hosp Infect, 82, pp.19~24 (2012)
- Weber DJ, "Understanding and preventing transmission of healthcare-associated pathogens due to the contaminated hospital environment", Infect Control Hosp Epidemiol, 34, pp.449~452 (2013)
- 김영아, "병원균의 병원환경오염과 감염관리", 의료관련감염관리, 20(1), pp.001~006 (2015), 대한의료관련감염관리학회
- Bae JY, "Sudden deaths of neonates receiving intravenous infusion of lipid emulsion contaminated with Citrobacter freundii", J Korean Med Sci, 33, pp.e97~ (2018)
- Dollwet HHA, "Historic uses of copper compounds in medicine", J Trace Elem Med, 2, pp.80~87 (1985)
- Kuhn PJ, "Door knobs: a source of nosocomial infection?", Diagnostic Medicine
- Kusumaningrum HD, "Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods", Int J Food Microbiol, 85, pp.227~236 (2003)
- Agarwala M, "Comparative study of antibiofilm activity of copper oxide and iron oxide nanoparticles against multidrug resistant biofilm forming uropathogens", Indian J Microbiol, 54, pp.365~368 (2014)
- Gould SWJ, "The antimicrobial properties of copper surfaces against a range of important nosocomial pathogens", Ann Microbiol, 59, pp.151~156 (2009)
- Carson KC, "In vitro susceptibility of methicillin-resistant Staphylococcus aureus and methicillinsusceptible Staphylococcus aureus to a new antimicrobial, copper silicate", Antimicrob Agents Chemother, 51, pp.4505~4507 (2007)
- 이은진, "식품제조 환경에서 식품위해세균의 교차오염 감소를 위한 청동합금 유기의 살균효과", 한국식품위생안전성학회지, 23(4), pp.309~313 (2008), 한국식품위생안전성학회
- Mikolay A, "Survival of bacteria on metallic copper surfaces in a hospital trial", Appl Microbiol Biotechnol, 87, pp.1875~1879 (2010)
- Casey AL, "Role of copper in reducing hospital environment contamination", J Hosp Infect, 74, pp.72~77 (2010)
- Jung MK, "Inhibitory effect of cupric ion diffused from brass ware on the growth of E. coli O157:H7, S. typhimurium, S. aureus, and B. cereus", Food Sci Biotechnol, 13, pp.680~683 (2004)
- CLSI, "Performance standards for antimicrobial susceptibility testing: twenty-third informational supplement. CLSI document M100-S23", Clinical and Laboratory Standards Institutes, ~ (2013)
- Weinstein RA, "Epidemiology and control of nosocomial infections in adult intensive care units", Am J Med, 91, pp.179S~184S (1991)