Prevalence of Helicobacter pylori infection and clarithromycin resistance rate from 2015 to 2018 using the laboratory information system of the Seegene Medical Foundation in Korea: a repeated cross-sectional study
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Background: Numerous studies have examined the prevalence of Helicobacter pylori infection and clarithromycin (CLA) resistance rate of H. pylori. However, in South Korea, there is a lack of research analyzing specimens from local clinics and hospitals using molecular methods. This study aimed to assess the prevalence of H. pylori infection and CLA resistance across sex and age groups, as well as to explore regional variations in CLA resistance and its characteristics. Methods: Data from a laboratory information system from 2015 to 2018 were retrospectively analyzed to determine the prevalence of H. pylori infection and CLA resistance rate. The 23S ribosomal RNA genes of H. pylori were analyzed using a dual priming oligonucleotide-based multiplex polymerase chain reaction method. Results: The overall prevalence of H. pylori infection was 50.5%(12,000/23,773), with a significantly higher prevalence among males (53.5%) than females (47.0%). The CLA resistance rate was 28.3%, with a significantly higher rate among females (34.9%) than males (23.8%). Age group analysis revealed that the highest prevalence of H. pylori infection was among individuals in their 40s, whereas the highest CLA resistance rate was observed among those in their 60s. The CLA resistance rate exhibited an upward trend and varied among patients based on their place of residence, and A2143G mutation was the most prevalent across all regions. Conclusion: The prevalence of H. pylori infection and CLA resistance rate in Korea remain high and vary according to sex, age, and region. To effectively eradicate H. pylori, it is crucial to periodically monitor regional CLA resistance patterns and conduct CLA susceptibility testing before prescription.
Abstract
Background: Numerous studies have examined the prevalence of Helicobacter pylori infection and clarithromycin (CLA) resistance rate of H. pylori. However, in South Korea, there is a lack of research analyzing specimens from local clinics and hospitals using molecular methods. This study aimed to assess the prevalence of H. pylori infection and CLA resistance across sex and age groups, as well as to explore regional variations in CLA resistance and its characteristics. Methods: Data from a laboratory information system from 2015 to 2018 were retrospectively analyzed to determine the prevalence of H. pylori infection and CLA resistance rate. The 23S ribosomal RNA genes of H. pylori were analyzed using a dual priming oligonucleotide-based multiplex polymerase chain reaction method. Results: The overall prevalence of H. pylori infection was 50.5%(12,000/23,773), with a significantly higher prevalence among males (53.5%) than females (47.0%). The CLA resistance rate was 28.3%, with a significantly higher rate among females (34.9%) than males (23.8%). Age group analysis revealed that the highest prevalence of H. pylori infection was among individuals in their 40s, whereas the highest CLA resistance rate was observed among those in their 60s. The CLA resistance rate exhibited an upward trend and varied among patients based on their place of residence, and A2143G mutation was the most prevalent across all regions. Conclusion: The prevalence of H. pylori infection and CLA resistance rate in Korea remain high and vary according to sex, age, and region. To effectively eradicate H. pylori, it is crucial to periodically monitor regional CLA resistance patterns and conduct CLA susceptibility testing before prescription.
참고문헌 (36)
- Malfertheiner P, "Helicobacter pylori infection", Nat Rev Dis Primers, 9, pp.19~ (2023)
- "WHO publishes list of bacteria for which new antibiotics are urgently needed"[URL]
- Hooi JKY, "Global prevalence of Helicobacter pylori infection : systematic review and meta-analysis", Gastroenterology, 153, pp.420~429 (2017)
- Liou JM, "Screening and eradication of Helicobacter pylori for gastric cancer prevention : the Taipei global consensus", Gut, 69, pp.2093~2112 (2020)
- Lee JH, "Nationwide antibiotic resistance mapping of Helicobacter pylori in Korea : a prospective multicenter study", Helicobacter, 24, pp.e12592~ (2019)
- Lee YC, "Association between Helicobacter pylori eradication and gastric cancer incidence : a systematic review and metaanalysis", Gastroenterology, 150, pp.1113~1124 (2016)
- Marques AT, "Trends in Helicobacter pylori resistance to clarithromycin : from phenotypic to genomic approaches", Microb Genom, 6, pp.e000344~ (2020)
- Nishizawa T, "Mechanisms of Helicobacter pylori antibiotic resistance and molecular testing", Front Mol Biosci, 1, pp.19~ (2014)
- Zou Y, "The effect of antibiotic resistance on Helicobacter pylori eradication efficacy : a systematic review and meta-analysis", Helicobacter, 25, pp.e12714~ (2020)
- Malfertheiner P, "Management of Helicobacter pylori infection—the Maastricht V", Florence consensus report, 2017, pp.66, 6~30
- Malfertheiner P, "Management of Helicobacter pylori infection : the Maastricht VI", Florence consensus report, 2022, pp.71, 1724~1762
- Pohl D, "Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing", World J Gastroenterol, 25, pp.4629~4660 (2019)
- Sholeh M, "The prevalence of clarithromycin-resistant Helicobacter pylori isolates : a systematic review and meta-analysis", PeerJ, 11, pp.e15121~ (2023)
- Yun JM, "Usefulness of dual priming oligonucleotidepolymerase chain reaction for diagnosis and treatment of Helicobacter pylori", Korean J Helicobacter Up Gastrointest Res, 16, pp.147~151 (2016)
- Lee HK, "Multicenter study for the frequency of 23S rRNA point mutations associated with clarithromycin resistance in Helicobacter pylori in Korea", Korean J Clin Microbiol, 11, pp.84~89 (2008)
- Kim T, "Clarithromycin-resistant Helicobacter pylori associated with 23S rRNA point mutations in Jeju Island", Korean J Gastroenterol, 61, pp.252~258 (2013)
- Cho AR, "A comparison analysis on the diagnosis of Helicobacter pylori infection and the detection of clarithromycin resistance according to biopsy sites", Korean J Lab Med, 30, pp.381~387 (2010)
- Jung MK, "The effect of concomittent therarpy and quadruple therapy for patients who had 23S ribosomal ribonucleic acid mutated Helicobacter pylori in Daegu and Kyoungpook area", Korean J Helicobacter Up Gastrointest Res, 14, pp.249~254 (2014)
- Jeon JS, "Detection of clarithromycin-resistant Helicobacter pylori by polymerase chain reaction using residual samples from rapid urease test", Indian J Med Microbiol, 35, pp.406~409 (2017)
- Chung WC, "Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection", World J Gastroenterol, 20, pp.6547~6553 (2014)
- Lee JH, "Seroprevalence of Helicobacter pylori in Korea : a multicenter, nationwide study conducted in 2015 and 2016", Helicobacter, 23, pp.e12463~ (2018)
- Lim SH, "Trends in the seroprevalence of Helicobacter pylori infection and its putative eradication rate over 18 years in Korea : a crosssectional nationwide multicenter study", PLoS One, 13, pp.e0204762~ (2018)
- Inoue M, "Changing epidemiology of Helicobacter pylori in Japan", Gastric Cancer, 20(Suppl 1), pp.3~7 (2017)
- Schröder W, "Gender differences in antibiotic prescribing in the community : a systematic review and meta-analysis", J Antimicrob Chemother, 71, pp.1800~1806 (2016)
- Kocsmár É, "Primary and secondary clarithromycin resistance in Helicobacter pylori and mathematical modeling of the role of macrolides", Nat Commun, 12, pp.2255~ (2021)
- De Francesco V, "Prevalence of primary clarithromycin resistance in Helicobacter pylori strains over a 15 year period in Italy", J Antimicrob Chemother, 59, pp.783~785 (2007)
- Chang YW, "Clarithromycin resistance and female gender affect Helicobacter pylori eradication failure in chronic gastritis", Korean J Intern Med, 34, pp.1022~1029 (2019)
- Almeida N, "Helicobacter pylori antimicrobial resistance rates in the central region of Portugal", Clin Microbiol Infect, 20, pp.1127~1133 (2014)
- Seo JH, "Comparison of the antibiotic resistance of Helicobacter pylori isolated in Jinju over a 15-year period", J Bacteriol Virol, 42, pp.305~312 (2012)
- De Francesco V, "Phenotypic and genotypic Helicobacter pylori clarithromycin resistance and therapeutic outcome : benefits and limits", J Antimicrob Chemother, 65, pp.327~332 (2010)
- Lin Y, "Antibiotic resistance in Helicobacter pylori : from potential biomolecular mechanisms to clinical practice", J Clin Lab Anal, 37, pp.e24885~ (2023)
- Mégraud F, "H pylori antibiotic resistance : prevalence, importance, and advances in testing", Gut, 53, pp.1374~1384 (2004)
- De Francesco V, "Primary clarithromycin resistance in Helicobacter pylori : the Multicentric Italian Clarithromycin Resistance Observational(MICRO)study", J Gastrointestin Liver Dis, 20, pp.235~239 (2011)
- De Francesco V, "Primary clarithromycin resistance in Italy assessed on Helicobacter pylori DNA sequences by TaqMan real-time polymerase chain reaction", Aliment Pharmacol Ther, 23, pp.429~435 (2006)
- Aguilar-Luis MA, "Highly clarithromycin-resistant Helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru", BMC Res Notes, 11, pp.809~ (2018)
- Mohammadi M, "Helicobacter pylori antibiotic resistance in Iran", World J Gastroenterol, 11, pp.6009~6013 (2005)