본문 바로가기
KERD
논문KCI 등재

Development and evaluation of surface plasmon resonance imaging for the detection of antibodies against classical swine fever virus in swine

2

조회

0

다운로드

0

인용

0

참고문헌

주제분류수의학

초록

Abstract

A protein chip based on surface plasmon resonance(SPR) imaging was developed for measuring classical swine fever virus(CSFV) antibody using a recombinant gp55 protein as an antigen. The diagnostic potential of SPR imaging for detecting antibodies to the CSFV gp55 protein was compared with that of a enzyme-linked immunosorbent assay(ELISA) using 70 pig sera. There was a strong positive correlation between the SPR imaging and ELISA(n=70, r=0.916, p<0.01). Therefore, the SPR imaging, which is a label-free and high-through put method, is expected to be a valuable tool in the serodiagnosis of CSFV.

참고문헌 (12)

  1. "Clinical signs and epidemiology of classical swine fever: a review of new knowledge", 165(1), pp.11~20 (2003)
  2. "Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999", 144(10), pp.421~429 (1999)
  3. "Enzyme-linked immunosorbent assay based on a chimeric antigen bearing antigenic regions of structural proteins Erns and E2 for serodiagnosis of classical swine fever virus infection", 12(7), pp.877~881 (2005)
  4. "Relation between lymphocyte subpopul-ations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex", 1(1), pp.49~52 (2000)
  5. "Application of competitive enzyme-linked immunosorbentassay for the serologic diagnosis of classical swine fever virus infection", 13(4), pp.357~360 (2001)
  6. "et al. 1993. Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera. J Virol 67", pp.5435~5442
  7. "et al. 1991. Structural proteins of hog cholera virus expressed by vaccinia virus fur-ther characterization and induction of protective immunity. J Virol 65", pp.589~597
  8. "Surface plasmon reso-nance imaging", 379(3), pp.328~331 (2004)
  9. "A fusion protein expression analy-sis using surface plasmon resonance imaging", 330(2), pp.251~256 (2004)
  10. "Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibodies of classical swine fever", 68(12), pp.1327~1329 (2006)
  11. "Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2", 70(4), pp.263~268 (2006)
  12. "Serodiagnostic comparison between two methods, ELISA and surface plasmon resonance for the detection of antibody titres of Mycoplasma hyopneumoniae", 53(2), pp.87~90 (2006)

관련 논문AI추천

이 간행물의 다른 논문 (12건)