닭 전염성빈혈 감염률 및 유전자 분석
Investigation of infection rate and genetic sequence analysis of chick infectious anemia virus
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Chicken anemia virus (CAV) has been recognized as an immunosuppressive agent and plays role as an etiological agent of multifactorial diseases in chicken. In this study, we investigated distribution of CAV antibody by ELISA and the virus gene by PCR in poultry farms in Jeongeup, Jeonbuk province. In the test using ELISA kit, 41 (95.3%) of 43 flocks and 88.6% of the individual chickens were positive, respectively. By PCR, 90.9% of the broiler breeders and 75.0% of White-semi breeders were found positive, respectively. All hatchery was negative by PCR. Of the clinical cases from 49 poultry flocks, 87.5% of flocks and 54.7% for each samples were found positive by ELISA, respectively. By PCR test, 21 (42.9%) of 49 flocks were positive. Major clinical signs of the infected flocks were growth retardation, femoral subcutaneous bleeding, depression, limping, and continuing selection. The genetic analysis of separate N genes of CAV showed highly homologous each other. The nucleotide sequence of field isolates had homology ranged from 99.9% to 97.5% with Chinese strains, and 99.9% to 99.6% with Japanese strain. Phylogenetic analysis based on the N gene of CAV isolates showed the closely relation with Chinese strains. The results of this survey could be used as basic data for development of vaccine.
Abstract
Chicken anemia virus (CAV) has been recognized as an immunosuppressive agent and plays role as an etiological agent of multifactorial diseases in chicken. In this study, we investigated distribution of CAV antibody by ELISA and the virus gene by PCR in poultry farms in Jeongeup, Jeonbuk province. In the test using ELISA kit, 41 (95.3%) of 43 flocks and 88.6% of the individual chickens were positive, respectively. By PCR, 90.9% of the broiler breeders and 75.0% of White-semi breeders were found positive, respectively. All hatchery was negative by PCR. Of the clinical cases from 49 poultry flocks, 87.5% of flocks and 54.7% for each samples were found positive by ELISA, respectively. By PCR test, 21 (42.9%) of 49 flocks were positive. Major clinical signs of the infected flocks were growth retardation, femoral subcutaneous bleeding, depression, limping, and continuing selection. The genetic analysis of separate N genes of CAV showed highly homologous each other. The nucleotide sequence of field isolates had homology ranged from 99.9% to 97.5% with Chinese strains, and 99.9% to 99.6% with Japanese strain. Phylogenetic analysis based on the N gene of CAV isolates showed the closely relation with Chinese strains. The results of this survey could be used as basic data for development of vaccine.
참고문헌 (22)
- 류광선, "Virus 중화시험법에 의한 닭 전염성 빈혈인자의 항체조사", 대한수의학회지, 33(2), pp.227~234 (1993)
- 성환우, "자염감염된 닭으로부터 chicken anemia agent (virus)의 분리", 대한수의학회지, 31(4), pp.471~477 (1994)
- Caterina KM, "Development of a multiplex PCR for detection of avian adenovirus, avian reovirus, infectious bursal disease virus, and chicken anemia virus", Mol Cell Probes, 18(5), pp.293~298 (2004)
- Craig MI, "Molecular characterization of chicken infectious anemia virus circulating in Argentina during 2007", Avian Dis, 53(3), pp.331~335 (2007)
- Davidson I, "The contribution of feathers in the spread of chicken anemia virus", Virus Res, 132(1), pp.152~159 (2008)
- Ducatez MF, "Molecular epidemiology of chicken anemia virus in Nigeria", Arch Virol, 151(1), pp.97~111 (2006)
- Gelderblom H, "Morphlolgical characterization of chicken anaemia agent", Arch Virol, 109(1), pp.115~120 (1989)
- Goryo M, "Isolation of an agent inducing chicken anaemia", Avian Pathol, 14(4), pp.483~496 (1985)
- Hoop RK, "Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens", Avian Pathol, 21(3), pp.493~501 (1992)
- Hoop RK, "Transmission of chicken anaemia virus with semen", Vet Rec, 133(22), pp.551~552 (1993)
- Huseyin HADiML H, "Investigation of Chicken Infectious Anemia Virus Infection by PCR and ELISA in Chicken Flocks", Turk J Vet Anim Sci, 32(2), pp.79~84 (2008)
- McNulty MS, "Chicken anaemia agent: a review", Avian Pathol, 20(2), pp.187~203 (1991)
- Otaki Y, "Isolation of chicken anaemia agent and Marek’s disease virus from chickens vaccinated with turkey herpesvirus and lesions induced in chicks by inoculating both agents", Avian Pathol, 16(2), pp.291~306 (1987)
- Rosenberger JK, "The effects of age, route of exposure and coinfection with infectious bursal disease virus on the pathogenicity and transmissibility of chicken anemia agent (CAA)", Avian Pathol, 33(4), pp.753~759 (1989)
- Roussan DA, "Serological Survey on the Prevalence of Chicken Infectious Anemia Virus in Commercial Broiler Chicken Flocks in Northern Jordan", Int J Poult Sci, 5(6), pp.544~546 (2006)
- Saif YM, "Diseases of poultry, 11th ed", pp.181~202 (2003), Iowa State University Press
- Toro H, "Effects of chicken anemia virus and infectious bursal disease virus in commercial chickens", Avian Dis, 53(1), pp.94~102 (2009)
- Toro H, "Chicken anemia virus and fowl adenoviruses: association to induce the inclusion body hepatitis", hydropericardium syndrome, 2000, pp.44(1), 51~58
- Toro H, "Pathogenicity of chicken anaemia virus (isolate 10343) for young and older chickens", Avian Pathol, 26(3), pp.485~499 (1997)
- Urlings HA, "Inactivation of chicken anaemia virus in chickens by heating and fermentation", Vet Q, 15(3), pp.85~88 (1993)
- van Santen VL, "Genetic characterization of chicken anemia virus from commercial broiler chickens in Alabama", Avian Dis, 45(2), pp.373~388 (2001)
- Yuasa N, "Isolation and some characteristics of an agent inducing anemia in chicks", Avian Dis, 23(2), pp.366~385 (1979)