닭에서 분리된 methicillin-resistant coagulase-negative staphylococci의 동정 및 staphylococcal cassette chromosome mec(SCCmec) type
Identification and staphylococcal cassette chromosome mec (SCCmec) type of methicillin-resistant coagulase-negative staphylococci isolated from chickens
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Methicillin-resistant coagulase-negative staphylococci (MRCNS) were isolated from the respiratory sites of chickens in 4 farms and slaughter house located in Chungnam provinces. Isolation of coagulase-negative staphylococci (CNS) was positive for 61 (26.6%) of the 229 chickens tested, and isolation of MRCNS was positive for 17 (27.9%) of the isolated CNS. A total of 17 MRCNS isolates were selected and subjected to identification. Of the 17 MRCNS isolates selected, 6 were identified as Staphylococcus cohnii, 2 as S. saprophyticus, 3 as S. simulans, 3 as S. lentus, 2 as S. carnosus, and 1 as S. xylosus. The MRCNS isolates were resistant to many beta-lactam antibiotics, and some isolates were also resistant to macrolide and aminoglycoside antibiotics. The mecA gene was detected in some isolates of each MRCNS strains. The mecA-positive isolates were classified into five staphylococcal cassette chromosome mec (SCCmec). SCCmec types I to IV were detected in isolates from chickens.
Abstract
Methicillin-resistant coagulase-negative staphylococci (MRCNS) were isolated from the respiratory sites of chickens in 4 farms and slaughter house located in Chungnam provinces. Isolation of coagulase-negative staphylococci (CNS) was positive for 61 (26.6%) of the 229 chickens tested, and isolation of MRCNS was positive for 17 (27.9%) of the isolated CNS. A total of 17 MRCNS isolates were selected and subjected to identification. Of the 17 MRCNS isolates selected, 6 were identified as Staphylococcus cohnii, 2 as S. saprophyticus, 3 as S. simulans, 3 as S. lentus, 2 as S. carnosus, and 1 as S. xylosus. The MRCNS isolates were resistant to many beta-lactam antibiotics, and some isolates were also resistant to macrolide and aminoglycoside antibiotics. The mecA gene was detected in some isolates of each MRCNS strains. The mecA-positive isolates were classified into five staphylococcal cassette chromosome mec (SCCmec). SCCmec types I to IV were detected in isolates from chickens.
참고문헌 (14)
- Aarestrup FM, "Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry", Vet Microbiol, 74(4), pp.353~364 (2000)
- Awan MA, "Heterogeneity of staphylococci and other bacteria isolated from six-week-old broiler chickens", Poult Sci, 77(7), pp.944~949 (1998)
- Eui Kyung Cha, "Correlation Between Staphylococcal Cassette Chromosome mec Type and Coagulase Serotype of Methicillin-Resistant Staphylococcus aureus", Journal of Bacteriology and Virology, 39(2), pp.71~78 (2009), 대한미생물학회
- Daki´c I, "Survey of genes encoding staphylococcal enterotoxins, toxic shock syndrome toxin 1, and exfoliative toxins in members of the Staphylococcus sciuri group", Clin Microbiol, 43(9), pp.4875~4876 (2005)
- Forder RE, "A small-scale, low-cost isolation system for the incubation and rearing of low bacterial load chicks as a model to study microbial-intestinal interactions", Lab Anim, 42(2), pp.185~192 (2008)
- Kaszanyitzky EJ, "Staphylococci isolated from animals and food with phenotypically reduced susceptibility to beta-lactamase-resistant beta-lactam antibiotics", Acta Vet Hung, 52(1), pp.7~17 (2004)
- Kawano J, "Isolation of methicillin-resistant coagulasenegative staphylococci from chickens", J Clin Microbiol, 34(9), pp.2072~2077 (1996)
- Kloos WE, "Stapylococcus and Micrococcus. in: Manual of clinical microbiology", pp.264~282 (1999), ASM Press
- Lindblad M, "Microbiological baseline study of broiler chickens at Swedish slaughterhouses", J Food Prot, 69(12), pp.2875~2882 (2006)
- Martins A, "Methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci: Epidemiological and molecular aspects", Microbiol Immunol, 51(9), pp.787~795 (2007)
- McNamee PT, "Bacterial chondronecrosis with osteomyelitis (‘femoral head necrosis’) of broiler chickens", Avian Pathol, 29(5), pp.477~495 (2000)
- Pepe O, "Staphylococcus aureus and staphylococcal enterotoxin A in breaded chicken products: detection and behavior during the cooking process", Appl Environ Microbiol, 72(11), pp.7057~7062 (2006)
- Skalka B, "Modern scheme for the diagnosis of staphylococci", Vet Med (Praha), 30(8), pp.477~484 (1985)
- Vuong C, "Staphylococcus epidermidis infections", Microbes Infect, 4, pp.481~489 (2002)
