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대구ㆍ경북지역 오리 유래 Salmonella속 균의 혈청형, 항균제 내성 및 S. Enteritidis와 S. Typhimurium의 plasmid profiles, phage types 및 PFGE

Serotypes, antimicrobial resistance of Salmonella spp. and plasmid profiles, phage types, PFGE of S. Enteritidis and S. Typhimurium isolated from ducks in Daegu-Gyeongbuk province

강민수|김기석|조재근
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2011.09|한국동물위생학회지|제34권 제3호|pp. 217-226

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참고문헌

키워드

SalmonellaPlasmidAntimicrobial resistancePFGEPhage type
주제분류수의학

초록

Salmonella spp. is of increasing public health concern as causative pathogens of food poisoning. The aim of this study was to investigate the serotypes and antimicrobial resistance pattern of Salmonella spp. isolated from duck farms in Daegu-Gyeongbuk province. Also, S. Enteritidis and S. Typhimurium isolates were further examined for plasmid analysis, phage typing and pulsed-field gel electrophoresis (PFGE). A total of 34 Salmonella spp.(16.4%) were isolated from duck farms and ten serotypes were identified. The predominant serotypes were S. Typhimurium(23.5%) S. Fyris(17.6%) and S. Haardt(11.8%), S. Agona and S. Enteritidis(respectively 8.8%). Of 34 Salmonella isolates, 15(44.1%) isolates were resistant to at least one antimicrobial agent and multiple resistance(resistance to more than 4 drugs) was observed in 9 strains(26.5%). The high resistance was found to streptomycin(32.4%), tetracycline(29.4%), ampicillin, kanamycin and nalidixic acid(respectively, 26.5%), all Salmonella isolates were susceptible to cefoxitin, cefotaxime, gentamicin, amikacin and ciprofloxacin. All S. Enteritidis and S. Typhimurium isolates were found to contain only one plasmid (ca. 54 or 55kb, respectively). Among the S. Enteritidis isolates, two phage types were found, PT32a and PT1c, respectively, one isolates did not react with any of the phages used. Whereas, all S. Typhimurium isolates were RDNC(reacts but does not conform). PFGE showed to be a useful typing method better than plasmid analysis and phage typing for discrimination of isolates especially, S. Typhimurium isolates. Our results indicated that the serotypes of Salmonella isolates are widely distributed in duck farms, further epidemiological studies should be carried out.

Abstract

Salmonella spp. is of increasing public health concern as causative pathogens of food poisoning. The aim of this study was to investigate the serotypes and antimicrobial resistance pattern of Salmonella spp. isolated from duck farms in Daegu-Gyeongbuk province. Also, S. Enteritidis and S. Typhimurium isolates were further examined for plasmid analysis, phage typing and pulsed-field gel electrophoresis (PFGE). A total of 34 Salmonella spp.(16.4%) were isolated from duck farms and ten serotypes were identified. The predominant serotypes were S. Typhimurium(23.5%) S. Fyris(17.6%) and S. Haardt(11.8%), S. Agona and S. Enteritidis(respectively 8.8%). Of 34 Salmonella isolates, 15(44.1%) isolates were resistant to at least one antimicrobial agent and multiple resistance(resistance to more than 4 drugs) was observed in 9 strains(26.5%). The high resistance was found to streptomycin(32.4%), tetracycline(29.4%), ampicillin, kanamycin and nalidixic acid(respectively, 26.5%), all Salmonella isolates were susceptible to cefoxitin, cefotaxime, gentamicin, amikacin and ciprofloxacin. All S. Enteritidis and S. Typhimurium isolates were found to contain only one plasmid (ca. 54 or 55kb, respectively). Among the S. Enteritidis isolates, two phage types were found, PT32a and PT1c, respectively, one isolates did not react with any of the phages used. Whereas, all S. Typhimurium isolates were RDNC(reacts but does not conform). PFGE showed to be a useful typing method better than plasmid analysis and phage typing for discrimination of isolates especially, S. Typhimurium isolates. Our results indicated that the serotypes of Salmonella isolates are widely distributed in duck farms, further epidemiological studies should be carried out.

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