개와 고양이에서 분리한 methicillin 내성 및 감수성 Staphylococcus pseudintermedius
Methicillin-resistant or susceptible Staphylococcus pseudintermedius isolates from dogs and cats
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Staphylococcus pseudintermedius is an important opportunistic pathogen of dog and cats. Since 2006 there has been a significant emergence of methicillin-resistant S. pseudintermedius (MRSP) mainly due to clonal spread. The aim of this study was to investigated the prevalence of antibiotic resistance and presence of mecA and femA gene in 91 S. pseudintermedius isolates isolated from dogs and cats asso-ciated with various clinic infections. Methicillin resistance was confirmed by oxacillin disc diffusion method. MRSP isolate was detected 19 isolates (20.9%). MRSP and methicillin-resistant S. pseudinter- medius (MSSP) isolates were highly resistant to penicillin, kanamycin, tetracycline, erythromycin, trime-thoprim-sulfamethoxazole, clindamycin, ciprofloxacin, enrofloxacin and choloramphenicol (100∼47.3% and 90.3∼33.3%, respectively). About 90% of MRSP isolates were multi-drug resistance (resistance to at least five or more antimicrobials), and MSSP isolates was ca 74%. Among the 91 isolates, mecA gene was detected in 25 isolates (27.5%, 19 in MRSP isolates and 6 in MSSP isolates), but none carried the femA gene. Our results indicated MRSA isolates show a strong resistance to antimicrobials com-monly used in veterinary medicine. A continuous surveillance and monitoring should be called for to prevent the contamination and spread of MRSP in dogs and cats.
Abstract
Staphylococcus pseudintermedius is an important opportunistic pathogen of dog and cats. Since 2006 there has been a significant emergence of methicillin-resistant S. pseudintermedius (MRSP) mainly due to clonal spread. The aim of this study was to investigated the prevalence of antibiotic resistance and presence of mecA and femA gene in 91 S. pseudintermedius isolates isolated from dogs and cats asso-ciated with various clinic infections. Methicillin resistance was confirmed by oxacillin disc diffusion method. MRSP isolate was detected 19 isolates (20.9%). MRSP and methicillin-resistant S. pseudinter- medius (MSSP) isolates were highly resistant to penicillin, kanamycin, tetracycline, erythromycin, trime-thoprim-sulfamethoxazole, clindamycin, ciprofloxacin, enrofloxacin and choloramphenicol (100∼47.3% and 90.3∼33.3%, respectively). About 90% of MRSP isolates were multi-drug resistance (resistance to at least five or more antimicrobials), and MSSP isolates was ca 74%. Among the 91 isolates, mecA gene was detected in 25 isolates (27.5%, 19 in MRSP isolates and 6 in MSSP isolates), but none carried the femA gene. Our results indicated MRSA isolates show a strong resistance to antimicrobials com-monly used in veterinary medicine. A continuous surveillance and monitoring should be called for to prevent the contamination and spread of MRSP in dogs and cats.
참고문헌 (28)
- Bardiau M, "Characterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats", Microbiol Immunol, 57, pp.496~501 (2013)
- Berger-Bächi B, "Factors influencing methicillin resistance in staphylococci", Arch Microbiol, 178, pp.165~171 (2002)
- Clinical Laboratory Standards Institute (CLSI), "Performance standards for antimicrobial discs and dilution susceptibility for bacteria isolated from animals, Approved Standards" (2008), National Committee for Clinical Laboratory Standards
- Detwiler A, "Multi-drug and methicillin resistance of staphylococci from canine patients at a veterinary teaching hospital", Vet Q, 33, pp.60~77 (2011)
- Devriese LA, "Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals", Int J Syst Evol Microbiol, 55, pp.1569~1573 (2005)
- Dziva F, "First identification of methicillin-resistant Staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in Trinidad, West Indies", Infect Ecol Epidemiol, 28, pp.29170~ (2015)
- Feng Y, "Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius in pets from South China", Vet Microbiol, 160, pp.517~524 (2012)
- Gortel K, "Methicillin resistance among staphylococci isolated from dogs", Am J Vet Res, 60, pp.1526~1530 (1999)
- Haenni M, "Characterisation of clinical canine meticillin-resistant and meticillin-susceptible Staphylococcus pseudintermedius in France", J Global Antimicrobial Resistance, 2, pp.119~123 (2014)
- Kadlec K, "Molecular analysis of methicillin-resistant Staphylococcus pseudintermedius of feline origin from different European countries and North America", J Antimicrob Chemother, 65, pp.1826~1828 (2010)
- 강민희, "Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs", Journal of Veterinary Science, 15(3), pp.409~415 (2014), 대한수의학회[DOI]
- Loeffler A, "First report of multiresistant, mecA-positive Staphylococcus intermedius in Europe: 12 cases from a veterinary dermatology referral clinic in Germany", Vet Dermatol, 18, pp.412~421 (2007)
- Moodley A, "Antimicrobial resistance in methicillin susceptible and methicillin resistant Staphylococcus pseudintermedius of canine origin: literature review from 1980 to 2013", Vet Microbiol, 171, pp.337~341 (2014)
- Nienhoff U, "Methicillin-resistant Staphylococcus pseudintermedius among dogs admitted to a small animal hospital", Vet Microbiol, 150, pp.191~197 (2011)
- Pellerin JL, "Epidemiosurveillance of antimicrobial compound resistance of Staphylococcus intermedium clinical isolates from canine pyodermas", Comp Immunol Microbiol Infect Dis, 21, pp.115~133 (1998)
- Perreten V, "Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study", J Antimicrob Chemother, 65, pp.1145~1154 (2010)
- Priyantha R, "Antimicrobial susceptibility of Staphylococcus pseudintermedius colonizing healthy dogs in Saskatoon, Canada", Can Vet J, 57, pp.65~69 (2016)
- Ruscher C, "Prevalence of Methicillin-resistant Staphylococcus pseudintermedius isolated from clinical samples of companion animals and equidaes", Vet Microbiol, 136, pp.197~201 (2009)
- Ruscher C, "Widespread rapid emergence of a distinct methicillin-and multidrug-resistant Staphylococcus pseudintermedius (MRSP) genetic lineage in Europe", Vet Microbiol, 144, pp.340~346 (2010)
- Sasaki T, "Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital", J Clin Microbiol, 45, pp.1118~1125 (2007)
- van Duijkeren E, "Review on methicillin-resistant Staphylococcus pseudintermedius", J Antimicrob Chemother, 66, pp.2705~2714 (2011)
- Wang Y, "Methicillin-resistant Staphylococcus pseudintermedius isolated from canine pyoderma in North China", J Appl Microbiol, 112, pp.623~630 (2012)
- Weese JS, "Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine", Vet Microbiol, 140, pp.418~429 (2010)
- Wettstein K, "Emergence of methicillin-resistant Staphylococcus pseudintermedius in Switzerland: three cases of urinary tract infections in cats", Schweiz Arch Tierheilkd, 150, pp.339~343 (2008)
- "Lancet", 2001, 1674~1675
- 유종현, "High Prevalence of Fluoroquinolone- and Methicillin-Resistant Staphylococcus pseudintermedius Isolates from Canine Pyoderma and Otitis Externa in Veterinary Teaching Hospital", Journal of Microbiology and Biotechnology, 20(4), pp.798~802 (2010), 한국미생물·생명공학회
- 윤장원, "Antibiotic Resistance Profiles of Staphylococcus pseudintermedius Isolates from Canine Patients in Korea", Journal of Microbiology and Biotechnology, 20(12), pp.1764~1768 (2010), 한국미생물·생명공학회
- Zubeir IE, "Phenotypic and genotypic characteristics of methicillin", oxacillin-resistant Staphylococcus intermedius isolated from clinical specimens during routine veterinary microbiological examinations, 2007, pp.121, 170~176