고속액체크로마토그래피를 이용한 가축용 호르몬제(고나도렐린, 프로게스테론, 옥시토신, 에스트라디올) 분석방법 개발
Development of new analytical methods using high performance liquid chromatography for animal hormones; gonadorelin, progesterone, oxytocin and estradiol
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초록
The aim of this study is to develop an optimal analytical method for gonadorelin, progesterone, oxytocin and estradiol, the major components of hormones. A relatively simple and reproducible method using high performance liquid chromatography was developed and as a result of the measurement of specificity, linearity, repeatability, accuracy and intermediated precision, the validity of the developed method was verified with the result of meeting the verification criteria of analytical method validation. Using this newly developed method, 12 post-market veterinary products were tested and the ingredient content were 91.9∼116.4%, which satisfied the 90∼120% condition of the administrative measure standard. Therefore, if the newly developed method is used for the collection examination of hormone in veterinary medicine, it can be useful as an approved test method.
Abstract
The aim of this study is to develop an optimal analytical method for gonadorelin, progesterone, oxytocin and estradiol, the major components of hormones. A relatively simple and reproducible method using high performance liquid chromatography was developed and as a result of the measurement of specificity, linearity, repeatability, accuracy and intermediated precision, the validity of the developed method was verified with the result of meeting the verification criteria of analytical method validation. Using this newly developed method, 12 post-market veterinary products were tested and the ingredient content were 91.9∼116.4%, which satisfied the 90∼120% condition of the administrative measure standard. Therefore, if the newly developed method is used for the collection examination of hormone in veterinary medicine, it can be useful as an approved test method.
참고문헌 (14)
- Abdel-Khalik J, "Simultaneous determination of endogenous steroid hormones in human and animal plasma and serum by liquid or gas chromatography coupled to tandem mass spectrometry", J Chromatogr B, 928, pp.58~77 (2013)
- Korean pharmacopoeia of veterinary medicinal products, "APQA", ~, pp.QIA notification No. 2016-16 (2016)
- Freeman RK, "The use of the oxytocin challenge test for antepartum clinical evaluation of uteroplacental respiratory function", Am J Obstet Gynecol, 121, pp.481~489 (1975)
- Heo S, "Effects of dietary energy and lysine intake during late gestation and lactation on blood metabolites, hormones, milk compositions and reproductive performance in primiparous sows", Can J Anim Sci, 88, pp.247~255 (2008)
- Hooker CW, "Effects of sex hormones upon body growth, skin, hair and sebaceous glands in the rat", Endocrinol, 32, pp.69~76 (1943)
- Kloet DE, "Hormones, brain and stress", Endocr Regul, 37, pp.51~68 (2003)
- "Tip for inspecting a veterinary pharmaceutical affair. Directive No.78" (2017)[URL]
- McNeil DE, "The first case under the WTO’s sanitary and phytosanitary agreement: the European union’s hormone ban", Va J Int’L, 39, pp.89~ (1998)
- Picard-Hagen N, "Effect of ganadorelin, lecirelin, and buserelin on LH surge, ovulation, and progesterone in cattle", Theriogenology, 84, pp.177~183 (2015)
- Sharpe RM, "Hormones and testis development and the possible adverse effects of environmental chemicals", Toxicol Lett, 120, pp.221~232 (2001)
- Somerville BW, "The role of estradiol withdrawal in the etiology of menstrual migraine", Neurology, 22, pp.355~ (1972)
- Vandenheuvel FA, "Precise ultramicro determination of steroid hormones by combined TLC and GLC analysis", J Am Oil Chem Soc, 42, pp.283~290 (1965)
- Woods, Stephen C, "Metabolic hormones and regulation of body weight", Psychol Rev, 81, pp.26~ (1974)
- Young SL, "Progesterone function in human endometrium : clinical perspectives", In semin Reprod Med, 28, pp.005~016 (2010)