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반려동물 유래 장내세균에서 plasmid 매개 퀴놀론 내성 유전자의 특성

Characterization of plasmid-mediated quinolone resistance genes in Enterobacteriaceae isolated from companion animals

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The aim of this study was to investigate the prevalence and characterization of plasmid-mediated quino-lone resistance (PMQR) gene in 79 Enterobacteriaceae isolated from dogs and cats. Of 79 isolates, PMQR genes were found in 10 (12.7%) isolates, including aac(6’)-lb-cr, qnrB, qnrS and qnrA detected alone or in combination in 8 (10.1%), 4 (5.1%), 2 (2.5%) and 1 (1.3%) isolates, respectively. Interest-ingly, two qnrS genes were detected in nalidixic acid and ciprofloxacin susceptible isolates. Extended- spectrum -lactamase (ESBL) was detected in 90% (9 isolates) of PMQR positives isolates. Among ESBL genes, CTX-M, TEM and SHV were detected in 9, 8 and 3 isolates, respectively. Almost all PMQR genes were detected in co-existence with ESBL genes. All PMQR positives isolates were multi-drug resistance (i.e. resistant to five or more antibiotics). qepA, OXA and CMY-2 genes were not found. The six transconjugants were obtained by conjugation experiment. The aac(6’)-lb-cr, qnrB and qnrS were co-transferred with CTX-M, TEM and/or SHV, whereas qnrA was not observed among transcon-ugants. This is the first report of the presence of aac(6’)-lb-cr and qnrA gene among Enterobacteriaceae isolates from dogs in Korea. The prudent use of antimicrobials and continuous monitoring for com-panion animals are required.

Abstract

The aim of this study was to investigate the prevalence and characterization of plasmid-mediated quino-lone resistance (PMQR) gene in 79 Enterobacteriaceae isolated from dogs and cats. Of 79 isolates, PMQR genes were found in 10 (12.7%) isolates, including aac(6’)-lb-cr, qnrB, qnrS and qnrA detected alone or in combination in 8 (10.1%), 4 (5.1%), 2 (2.5%) and 1 (1.3%) isolates, respectively. Interest-ingly, two qnrS genes were detected in nalidixic acid and ciprofloxacin susceptible isolates. Extended- spectrum -lactamase (ESBL) was detected in 90% (9 isolates) of PMQR positives isolates. Among ESBL genes, CTX-M, TEM and SHV were detected in 9, 8 and 3 isolates, respectively. Almost all PMQR genes were detected in co-existence with ESBL genes. All PMQR positives isolates were multi-drug resistance (i.e. resistant to five or more antibiotics). qepA, OXA and CMY-2 genes were not found. The six transconjugants were obtained by conjugation experiment. The aac(6’)-lb-cr, qnrB and qnrS were co-transferred with CTX-M, TEM and/or SHV, whereas qnrA was not observed among transcon-ugants. This is the first report of the presence of aac(6’)-lb-cr and qnrA gene among Enterobacteriaceae isolates from dogs in Korea. The prudent use of antimicrobials and continuous monitoring for com-panion animals are required.

참고문헌 (34)

  1. Aslantaş Ö, "Prevalence and molecular characterization of extended-spectrum β-lactamase(ESBL)and plasmidic AmpC β-lactamase(pAmpC)producing Escherichia coli in dogs", J Vet Med Sci, 79, pp.1024~1030 (2017)
  2. Bradley DE, "Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12", J Bacteriol, 143, pp.1466~1470 (1980)
  3. Briales A, "Prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in Escherichia coli and Klebsiella pneumoniae producing extendedspectrum β-lactamases in Spain", Int J Antimicrob Agents, 39, pp.431~434 (2012)
  4. Briñas L, "Mechanisms of resistance to expanded-spectrum cephalosporins in Escherichia coli isolates recovered in a Spanish hospital", J Antimicrob Chemother, 56, pp.1107~1110 (2005)
  5. Briñas L, "Beta-lactamases in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals", Antimicrob Agents Chemother, 46, pp.3156~3163 (2002)
  6. Cantas L, "High emergence of ESBL-producing E. coli cystitis : time to get smarter in cyprus", Front Microbiol, 6, pp.1446~ (2015)
  7. Cavaco LM, "Evaluation of quinolones for use in detection of determinants of acquired quinolone resistance, including the new transmissible resistance mechanisms qnrA, qnrB, qnrS, and aac(6')Ib-cr, in Escherichia coli and Salmonella enterica and determinations of wild-type distributions", J Clin Microbiol, 47, pp.2751~2758 (2009)
  8. 조재근, "대구지역 동물병원에서 입원중인 개와 고양이로부터 분리된 항생제 내성 대장균", 한국동물위생학회지, 40(3), pp.193~200 (2017), 한국동물위생학회
  9. CLSI. Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial disk and dilution susceptibility tests for bacterial isolated from animals; approved standard. fourth edition and supplement, CLSI document VET01-A4 (standard) and VET01-S2 (supplement)" (2013), Clinical and Laboratory Standards Institute
  10. Hooper DC, "Emerging mechanisms of fluoroquinolone resistance", Emerg Infect Dis, 7, pp.337~341 (2001)
  11. Ishida Y, "Molecular analysis of antimicrobial resistance in gram-negative bacteria isolated from fish farms in Egypt", J Vet Med Sci, 72, pp.727~734 (2010)
  12. Jacoby GA, "Mechanisms of resistance to quinolones", Clin Infect Dis, 41, pp.S120~S126 (2005)
  13. Jacoby GA, "Temporal appearance of plasmid-mediated quinolone resistance genes", Antimicrob Agents Chemother, 53, pp.1665~1666 (2009)
  14. 강철인, "다제내성 그람음성균 감염의 항생제 치료", 대한내과학회지, 88(5), pp.502~508 (2015), 대한내과학회[DOI]
  15. Kim HB, "Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period", Antimicrob Agents Chemother, 53, pp.639~645 (2009)
  16. Liao CH, "Risk factors and clinical characteristics of patients with qnr-positive Klebsiella pneumoniae bacteraemia", J Antimicrob Chemother, 68, pp.2907~2914 (2013)
  17. Liu X, "High prevalence of β-lactamase and plasmid-mediated quinolone resistance genes in extended-spectrum cephalosporin-resistant Escherichia coli from dogs in Shaanxi, China", Front Microbiol, 16, pp.1843~ (2016)
  18. Liu X, "Occurrence of OXA-48 carbapenemase and other β-lactamase genes in ESBLproducing multidrug resistant Escherichia coli from dogs and cats in the United States, 2009-2013", Front Microbiol, 7, pp.1057~ (2016)
  19. Ma J, "High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6')-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals", Antimicrob Agents Chemother, 53, pp.519~524 (2009)
  20. Pagani L, "Multiple CTX-M-type extended-spectrum beta-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy", J Clin Microbiol, 41, pp.4264~4269 (2003)
  21. Park YJ, "Prevalence and diversity of qnr alleles in AmpC-producing Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii and Serratia marcescens : a multicentre study from Korea", J Antimicrob Chemother, 60, pp.868~871 (2007)
  22. Poirel L, "Plasmid-mediated quinolone resistance; interactions between human, animal, and environmental ecologies", Front Microbiol, 3, pp.24~ (2012)
  23. Poirel L, "Association of plasmid-mediated quinolone resistance with extended-spectrum beta-lactamase VEB-1", Antimicrob Agents Chemother, 49, pp.3091~3904 (2005)
  24. Robicsek A, "The worldwide emergence of plasmid-mediated quinolone resistance", Lancet Infect Dis, 6, pp.629~640 (2006)
  25. Robicsek A, "Fluoroquinolonemodifying enzyme : a new adaptation of a common aminoglycoside acetyltransferase", Nat Med, 12, pp.83~88 (2006)
  26. Robicsek A, "qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States", Antimicrob Agents Chemother, 50, pp.2872~2874 (2006)
  27. Rodríguez-Martínez JM, "Plasmid-mediated quinolone resistance : an update", J Infect Chemother, 17, pp.149~182 (2011)
  28. 신동호, "환축에서 분리된 Escherichia coli의 plasmid-mediated quinolone resistance genes 분포도 조사", 생명과학회지, 20(6), pp.964~967 (2010), 한국생명과학회
  29. So JH, "Dissemination of multidrug-resistant Escherichia coli in Korean veterinary hospitals", Diagn Microbiol Infect Dis, 73, pp.195~199 (2012)
  30. Tamang MD, "Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea", Antimicrob Agents Chemother, 56, pp.2705~2712 (2012)
  31. Tian GB, "Detection of clinically important β-lactamases in commensal Escherichia coli of human and swine origin in western China", J Med Microbiol, 61, pp.233~238 (2012)
  32. Webber M, "Quinolone resistance in Escherichia coli", Vet Res, 32, pp.275~284 (2001)
  33. Yu T, "Detection of extended-spectrum β-lactamase and plasmid- mediated quinolone resistance determinants in Escherichia coli isolates from retail meat in China", J Food Sci, 80, pp.M1039~M1043 (2015)
  34. Zhao X, "Decreased quinolone susceptibility in high percentage of Enterobacter cloacae clinical isolates caused only by qnr determinants", Diagn Microbiol Infect Dis, 67, pp.110~113 (2010)

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